GFP DNA in BBa_K823023 was transformed in B. subtilis. Colonies were visible on plate and a colony PCR was performed to check the insert. Insert was of the size of Psalt + GFP.
Template:Team:Groningen/CONTENT/LOGBOOK/Psalt GFP Transformation zp80
Psalt GFP Transformation (zp80)
A Transformation is done to get the desired DNA into B. subtilis ComI.
To create a salt inducible promotor.
Transformation was successful.
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/Transformation">Transformation</a>
00:00, 31 August 2015 - 00:00, 31 August 2015