Team:Colegio EmelinaU/Notebook

Journal of Laboratory

May 30th, 2015

 Genetic transformation of red coloration [B0010]  Plasmid removing: [K909008]= tetR-DBD-UVR8 [B0010]= Terminator [R0040]= Promoter

June 13th, 2015

* Genetic transformation of [ B0010 ] * failed . We make a new attempt • Electrophoresis : [ K909008 ] [ B0010 ] [ R0040 ]

June 20th, 2015

• Expanded coloring [ B0010 ] • Make plasmid removing [ B0010 ] • Perform digests [ B0010 ] = ( Terminator ) Digestion [ K909008 ] = ( tetR - DBD - UVR8 ) Double digestion • electrophoresis : [ B0010 ] 16746p [ K909008 ] 21506p

June 27th, 2015

Purification : [ B0010 ] [ K909008 ] • Plasmid removing : [ E1010 ] • Electrophoresis : [ B0010 ] [ K909008 ] [ E1010 ]

July 04th, 2015

 Plasmid removing: [J23119] [B0010] [K909008] [R0040] [E1010]  Electrophoresis: [B0010] [R0040] [K909008] [J23119]  Digestion: [B0010] [K909008]

July 13th, 2015

 Electrophoresis  Plasmid Removing  Terminator digestion and teR-DBD-UVR8

July 14th, 2015

 Bands Purification: [K909008] [B0010]  Join [K909008] [B0010]  Electroophoresis:July04th= [K909008];[J23119];[B0010];[R0040] [K909008]; [R0040]; [B0010]; [R0040]  Digestion

July 24th, 2015

 Digestion= [K909008]= Xbal-PSTI [J23119]= SPEI-PSTI [B0010]= EcoRI-Xbal [R0040]= SPEI-PSTI [E1010]= Xbal-PSTI

August 1st, 2015

 Digestion: [B0010]= E X I [R0040]= S P [K909008]= X P II [J23119]= SPEI PSTI [R0040]= S P I [R0040]= S P I [K909008]= X P II N [B0010]= E X I  Genetic Transformation: [J23119]= constitutive promoter [K1084103]= RBS [K909008]= tetR-DBD-UVR8 [B0010]= Terminator [R0040]= Promoter [K592009]= purple color [K592011]= blue color

August 22nd, 2015

 Digestion: [K1084103]  Purification: [K909008]  Genetic transformation: [K592009] August 29th, 2015  Plasmid removing: [K592009]  Purification: [K909008] Black [K909008] blue