Team:London Biohackspace/lab-book

Protocols

SLiCE/Ex vivo DNA Assembly i.e. Cheap Gibson

Filtration method for competent cells preparation

Preparing liquid YEPD media

Preparing YEPD agar plates

Electroporation Transformation of E. coli DH5aplha

Electroporation Transformation of S. cerevisiae

Experiments

SLiCE/Ex vivo DNA Assembly i.e. Cheap Gibson

Expressing Miraculin in S. cerevisiae

Expressing Miraculin in S. cerevisiae

RNA interference based regulation of RFP in E. coli

Expressing Lycopene in E.coli and S. cerevisiae

Creating Leucine auxotrophic S. cerevisiae strains