Team:Harvard BioDesign/Notebook
Our 2015 Lab Notebook!
Week of 6/1
First week in lab! Microbiology boot camp. How to pour plates, PCR, miniprep, gel electrophoresis. Researching project ideas.
Week of 6/8
Settle on bacterial adhesion. Further background research. Plasmid design, primer design. Choose fusion peptides. Order gBlocks from IDT of Rhamnose, Lac, and Arabinose promoters.
Week of 6/15
PCR backbones and fim proteins. Pour Chlor and Amp plates. Gibson assembly of operon plasmid and fimH plasmid. Pili purification assay development. NEGEM Meetup #1.
Week of 6/22
Redo failed PCRs. Redo failed Gibsons. Transform and plate operon Gibson. Pili purification and agglutination assay development. Microscopy on control strains.
Week of 6/29
Miniprep operon plasmid. Sent for sequencing. Unsuccessful- redo operon PCRs.
Week of 7/6
Induction. Operon Gibson. Agglutination assay development.
Week of 7/13
Induction. Edit out illegal PstI cut site from fimH. Mutagenesis to add binding peptides.
Week of 7/20
Induction. Transform measurement study parts. Mutagenesis to add binding peptides.
Week of 7/27
Induction. Nickel binding assay. Made biobricks for pRha-fimH and fimH. PCR-ing out from parts we were using with low-copy backbone, Gibson with pSB1C3.
Week of 8/2
Induction. Induction. More Induction. Nickel binding assay. Stainless steel binding assay. Cancer binding assay. Made biobricks, sent for sequencing.
Week 8/9
Induction. Induction. More Induction. Nickel binding assay. Stainless steel binding assay. Cancer binding assay. Retried failed biobricks.
Week of 8/16
Break from lab.
Week of 8/23
Break from lab.
Week of 8/30
Wiki and presentation planning.
Week of 9/6
Retried failed biobricks. Obtained results for Rhamnose-GFP inductions. Made presentation. NEGEM Meetup #2!
Week of 9/13
Worked on parts pages. Worked on wiki.
9/24 - 9/28
iGEM Giant Jamboree!!!
