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Parts

Natural halophilic protein linker 1

CGGCGAGAGAACATGCTGTGGTTTCGAGTGCCC

Natural halophilic protein linker 2

CGAGCCGAGAACATGCTGTGGCACAAACTTCCC

Engineered rigid halophilic protein linker

GAAGCTGCTGCTAAAGAAGCTGCTGCTAAA

Engineered flexible halophilic protein linker

GGAGGAGGAGGAAGTGGAGGAGGAGGAAGT

Codon Optimized Shewanella Sediminis Alcohol Dehydrogenase for expression in Haloferax Volcanii

ATGACGGTCACGAACGAGCAAGAGCTCGATCTGCTGGTCCAGCGAGTGGCCGATGCGCAG GCCGAGTACGCCAATTTCTCGCAGGAGCAGGTGGACAAGATTTTCCGAGCGGCGGCGCTG GCGGCCGCGGATGCCCGGATCAGCCTCGCGAAGATGGCGGCGAATGAAACGGGCATGGGC GTGATCGAGGACAAGGTCATCAAGAATCACTTCGCCTCGGAGTACATTTACAACAAGTAC AAGGATGAGAAAACCTGTGGCATTCTGGACGAGGATCCCACGTTCGGCACGATCACCATC GCGGAGCCGGTCGGTATCATTTGCGGGATCGTCCCGACGACGAACCCCACCTCCACGGCG ATCTTTAAGGCCCTGATTTCGCTCAAGACCCGCAACGGCATCATCTTCTCGCCGCACCCC CGAGCCAAGGAGTCCACCACGACGGCGGCCCGGCTCGTCCTGAATGCCGCGATCGAAGCG GGTGCGCCCAAGGATATTATCGGGTGGATCGATGAGCCGTCGGTGGCCCTGTCGAACCAG CTCATGACGCATGATAAGATTAACCTCATTCTCGCCACCGGCGGTCCCGGTATGGTGAAG GCCGCCTACTCGTCGGGGAAGCCGGCCATTGGGGTGGGTGCCGGGAACACCCCCATCGTC ATTGACGAAACCGCGGACATCAAGCGCGCGGTGTCGTCCATCCTCATGTCCAAGACCTTT GACAACGGGGTGGTGTGCGCGTCGGAGCAAGCCGTGGTCATTGTCGACGAAATTTACGAT ACGGTCAAGGAGCGGTTTGCCACGCACGGGGGCTACATTCTCAGCAAAGACGAGACCGCC GCCATGCAAAATGTCATTCTGAAGAATGGCGGGCTCAACGCGGATATCGTCGGTCAGAGC GCCGTGACCATTGCGGCGATGGCCGACATTGAGGTCCCCTCGTGGACGAAGGTCCTGATT GGTGAGGTCGAGGATGTCTCGGAGGCGGAGGCCTTTGCGCATGAGAAGCTCAGCCCGCTG CTGGGCATGTACCGCGTGAAGGACTTTGAAGCCGCCATGGACAAAGCCGAAGCCCTGGTC ACCCTCGGGGGTATCGGTCACACGAGCGGTCTGTACACGAACCAAGACACGCAGACGGAA CGCGTGATGGCGTTTGGCTTCCGCATGAAGACGGCGCGCATCCTGATCAACACGCCGGCG TCCCAAGGTGGTATTGGCGACCTGTATAACTTTAAGCTGGCCCCCTCGCTCACGCTGGGC TGCGGCAGCTGGGGTGGGAATTCGATTAGCGAGAACGTCGGCCCGTCGCACCTCATCAAT AAGAAAATGGTCGCGAAACGCGCGGAGAACATGCTCTGGCACAAGCTCCCGTCGTCGATC TACTTCCGACGGGGGTCGCTCCCGATTGCCCTCGAAGAACTCAGCGATAAAAAGCGAGCG CTGATCGTCACGGATAAGTACCTCTTTAATAACGGCTATTGCGATGAGACCATCAAAATT CTCAAGTCGCAGGGGCTCGAAACGGAGATCTTCTACGAAGTGGAAGCGGACCCGACGCTC GCGATTGTCACGCAGGGCGCGAAGATGGCCCAATCGTTCCAGCCGGATGTGATCATTGCC CTGGGTGGGGGCAGCCCGATGGATGCCGCCAAGATTATTTGGGTCATGTACGAGCACCCG GACGTCGATTTTGCGGACCTCGCCCTCCGGTTTATGGACATTCGCAAGCGCATCTACAAG TTCCCGAAACTGGGGGTCAAAGCGCAGATGGTGGCCATCCCGACCACGTCGGGCACCGGC AGCGAGGTGACGCCGTTCGCGGTCGTCACCGATGAAAAGACGGGTATGAAGTACCCCATT GCCGATTATCAACTGACGCCCAACATGGCGATCGTGGATCCGAACCTGGTGATGGATATG CCGAAGTCGCTGACCGCCTTCGGGGGGATTGACGCGGTGACCCACGCGCTCGAGGCCTAC GTCTCGGTCATGGCCAACGAATACTCGGACGGCCAGGCGCTCCAAGCGCTGGATCTCCTC TTCAAGCACCTCCCGGACAGCTACAACCACGGTGCCGCGGCGCCCATCGCCCGGGAAAAG GTCCACAATGGCGCGACGATTGCGGGGATCGCGTTCGCCAACGCCTTCCTCGGGATTTGT CACAGCATGGCCCATAAGCTGGGGGCCGAATTTCACCTGGCCCACGGGCTCGCCAATGCC CTGCTCATTAACAACGTCATCCGGTTCAACGCGACGGACATGCCGACGAAGCAGGCGGCG TTTTCGCAGTACGATCGACCCAAGGCGCTGTGCCGCTACGCGAAAATCGCCGAATATCTG AACCTGGGCGGTACGACGGATGAGGAGAAGGTGGAGGCCCTCATTGAAGAAGTCGACCAG CTGAAAAAAACCATTGGTATTCCGGCGAGCATTCGAGAGGCGGGTGTCAACGAGGCCGAC TTTCTGGCCAAACTCGATGAACTCGCCGAGGACGCGTTTGACGATCAATGTACGGGCGCC AATCCGCGCTACCCGCTGATTGAGGAACTGAAGCAAGTCCTCCTCGCGTCGTTCTACGGC
ACGGCCTATTCGGACAAGTGA

Codon optimized Zymomonas mobilis pyruvate decarboxylase for expression in Haloferax Volcanii

ATGTCGTACACGGTCGGCACGTACCTCGCCGAGCGCCTCGTCCAGATCGGCCTCAAACAC CACTTCGCGGTGGCCGGAGATTACAACCTGGTCCTGCTCGACAACCTCCTCCTCAACAAA AACATGGAGCAGGTCTACTGCTGCAACGAACTGAATTGCGGTTTTTCCGCCGAGGGCTAC GCGCGAGCGAAAGGTGCCGCGGCGGCGGTCGTGACGTACTCGGTCGGGGCCCTGTCGGCC TTCGACGCGATCGGAGGAGCGTATGCGGAGAACCTCCCCGTCATCCTGATCTCGGGTGCG CCGAATAACAACGATCATGCCGCCGGTCACGTCCTCCACCACGCCCTGGGCAAGACGGAT TACCACTACCAGCTCGAAATGGCGAAGAACATTACCGCCGCCGCGGAAGCCATCTACACG CCCGAAGAGGCGCCGGCCAAGATTGATCATGTCATTAAGACGGCCCTGCGAGAGAAAAAG CCGGTCTACCTCGAGATCGCCTGTAACATTGCCTCGATGCCGTGTGCCGCCCCCGGTCCC GCGTCGGCGCTCTTCAATGATGAAGCCTCGGACGAAGCGTCGCTCAATGCGGCCGTCGAG GAGACGCTGAAATTCATCGCCAACCGCGACAAGGTGGCGGTCCTCGTCGGCTCGAAGCTG CGAGCCGCGGGTGCCGAGGAAGCCGCCGTCAAGTTCGCGGATGCGCTGGGGGGAGCGGTC GCCACGATGGCGGCCGCGAAAAGCTTCTTCCCGGAAGAGAACCCGCACTATATCGGTACG TCGTGGGGTGAGGTCTCCTATCCGGGTGTGGAGAAGACGATGAAGGAGGCGGACGCGGTC ATCGCGCGCTCGCCCCGGTGTTCAACGACTACAGCACCACGGGCTGGACGGACATCCCGG ACCCGAAAAAACTCGTGCTCGCGGAGCCGCGATCCGTCGTCGTGAATGGCATTCGCTTCC CGTCGGTCCACCTCAAGGACTACCTCACGCGCCTGGCCCAAAAGGTCTCGAAGAAAACCG GAGCCCTGGACTTCTTCAAGTCGCTGAATGCCGGTGAACTCAAGAAAGCGGCGCCCGCGG ATCCCTCCGCCCCGCTCGTGAACGCGGAAATCGCCCGCCAGGTCGAAGCGCTCCTGACGC CGAACACGACGGTGATTGCCGAGACGGGCGACTCGTGGTTCAATGCCCAACGCATGAAGC TCCCGAACGGAGCGCGGGTCGAATACGAGATGCAGTGGGGCCACATCGGCTGGTCGGTGC CGGCCGCCTTCGGCTATGCGGTGGGAGCCCCGGAACGGCGAAATATTCTCATGGTCGGCG ACGGCTCGTTTCAGCTCACGGCGCAGGAGGTCGCCCAGATGGTCCGACTGAAACTCCCGG TCATTATCTTCCTCATCAACAACTACGGCTACACGATCGAGGTCATGATCCATGATGGGC CCTACAACAACATCAAAAACTGGGACTACGCGGGCCTGATGGAAGTCTTCAACGGCAATG GCGGATACGACAGCGGTGCGGGCAAGGGTCTCAAAGCGAAAACGGGTGGCGAACTGGCCG AGGCCATCAAGGTCGCGCTGGCGAATACGGATGGCCCGACGCTCATCGAGTGCTTCATTG GCCGCGAAGACTGCACGGAAGAACTCGTCAAATGGGGCAAGCGCGTGGCCGCCGCCAATA
GCCGCAAACCGGTCAACAAGCTCCTCTGA

Codon Optimized Haloarcula Hispanica beta-glucosidase

ATGGCACATGACACGACTGACGACGGTATGCAGGCGTCTCGGCGGACATTTCTGAAAGCG ACCGGTGTGGCCACGGCAGCGGCCGCCACCGGCGCAGGTGTTGGGACCGCCGCGGCACAA CCGGACGTCGACAGTCTCATTGGCGACCTCACCCTCGAGCAGAAAGCGGCCCAGATGACG CAGGTCGCGATCTCGTCGTTCGAAGCCGAACCGGAAGAGTCGAATGTGCCGGATTCGTTT GGGGTCGACACGGTCGGCGAGTACTTCAGCGAGCTGGGCATCGGGTCGATCCTGTCGGGT GGCGCGGAACCGCCGTCGTTTGACGCGAACGCGGTCGTCCAGGGGATCAACGCCCTGCAG CAATACAACCTCGACAACGCGGACCACGACATCCCGTTCCTCTACGGGGTGGATGCGACG CACGGCAACGGCCTCCTGGAAGGGGCCACCGTCTTCCCGCAACGCCTCAACATGGGCGTC ACGCGAGATCTGTCGCTCATCGCCGAAGCGGAACGCCACACGTCGGACGCCACGGCGAGC ATGGGTGCCCATTGGACCTTTGCGCCGACGACGGACCTCCAACGCGACCCGCGCTGGGGC CGATTTTTCGAAGGCATCTCGGAAGACCCGAAGCTGGAGGCGGATGTCAGCCGAGCCCGA GCGCGAGCCCTCGAAGACGACGATCGCCTCACGGCCTGCGTGAAGCACTTCGCGGCGTAC TCGGTGCCGAACAATGGCAATGATCGAGCGCCCGCCAGCACGAGCCTCCGAGACCTCCGC ACGAACATCCTCCCGCCGTACCGAGAGGCGCTGGAATCGGAGCCCGGCACGGTCATGGTC AACTCGGGTAGCATCAACGGCGTGCCCGCCCACGCCTCCCACTGGCTCCTCACGACGCTC CTCCGCGACACGTACGGCTACGAGGGGATGGTCGTGTCGGATTGGGACGATCTCAATCGC ATGATCACGAATCACGACTATGCCCCCGATTTCGAGACGGCGACGGAGATGGCGATCAAT GCGGGCGTCGATATGTATATGATCGGTAACGGTGGGGATGCCCCGGGTCCCGTCCAATTC ATCGACACCGTGGTGTCGCTGGTCGAGGACGGTGCGATCCCGATGGAGCGAATCGACGAG GCGGTCCGCCGCATTCTCGAGCTCAAGGCCGACCTGGGCCTCTTTGAACAACCGACGGTC GACGAATCGCGAATTGAGACGGTCCTGGGTGGTGCGCAGGAGACCGCCGAGACGATGGCG AAAGAGTCGATGGTGCTCCTGAAGAACACCGACGATACGCTCCCGCTCTCGGGCGATGAG TCCGTCCTCCTGACGGGTCCGGGTGTGGACTCGAATGGCAATAATACGCGAGCCCTCATG CAGCATGGTGGTTGGACGCTCGGCTGGCAAGGCGCCAGCGCCGGTGGCCCGTTTCCGCGC CAGAACCTGCTGGAGGCCGAACTCCGAGCGCGAGTCGGCTCCCTCACGCACGTCCCGACG AGCTACGAGAATACCACCTGGTGGGCGGGTGAAGGCGACGGCGAGAACCAGCAGTCGGAT GAAAATGGTAATTTCGACTTCACGGCGGAGCAGCGCTCGCGGGTCGAATCCGCCGGTCCC GAATCGGATGTGGTGGTCGTGGTCCTGGGGGAAGGCACGCACAACGAGGGCTTCGGCGAC CGAGATGAACTCGTGCTCGACGAGTCGCAGCAGGCGCTCCTCGATACCGTGGTGGAATCG ACGGATGACAGCACGCCGATCATCGGGGTCATGCTGGCCGGCTCGCCCCGAGGCTCCCCC GAGACGTTTAGCCAGCTCGATGCCCTCCTCTTTGCGGGTCAACCGGGTAGCGACGGGGGT GTCGCGATTGCGGAGACGCTGGTCGGCGAGTACAACCCCTCGGGTAAGCTCCCGTTCTCG TGGCCGGAGAATGTCGGCACGACCCCGGTCCAGTACACGCGCTACGACCCGACGTCCACG GGTGGTACGGACAACACGGCGATCTACGAATATGGGCACGGCCTGTCGTACACGGATTTC GAGTACGGCTCGGTCAGCGTGACGCAGCCGACCGTCGGCAGCCCGGCCAACCAATCCGAG GTCACCGTGTCGGTGGAGGTGACGAACACGGGTCGCATGGCCGGTGAGCACATTGTCGAG GTCTTCAACACGCAGTCGTATGGGACGGTCCTCCAACCGCGACGACGACTCCTCGGTTAC GAACGCGTCTCGCTGGGTCCCGGGGAACTGACGACCGTGGATGTCACGGTGGATCTCACG GCCCTGGAAGTGGTGCCCGGTGACGTGCTGGGCCTCGGTCCGAAGGTCGTCGAAGCGGGG GAATACGAGCTGACGGTGGGCCAAGACGGCCCGACGGCCACCCTCACCGTCCAGAATACG GCGTCGATCACGGATCCCGATCCGGTGCCGGGTCTGCCGGACTGGTCCGGCTCCACGCCC GGTCGCGGCAACTCGGGCAACAACGGCAACGGTAACAATACGTCGCGAGACCCCACGATG
GAGGATGTCGTGGACCTCCTGGATGAGGTCCGCGGTCGCTCGTAG

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