Team:Harvard BioDesign/Description

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We used the titratable Rhamnose promoter BBa_K902065 in our fimH plasmids, and were able to improve its characterization. Preliminary results in our agglutination assays led us to hypothesize that the promoter was leaky. There is some question is to whether Rhamose is actually titratable. That is, the effect cross the population of the inducer appears to be aincrasing linearly with the under. But there was evidence in the literature, that t single cell elver, the cell was either completely induced or completely induced. So it appears to be tithable on a population level, but you'r actually seeing no expression over some subset and 100% of another. We wanted to see if that was the case for the iGEM repost, wWe found an existing part that contained the Rhamnose promoter expressing GFP, and we induced with varying concentrations of RHmnos, for very small to a lot of Rhamnose. We perfumed flow symmetry to measure th e fluorescence of individual cells, and shows the distribution of the fluorescence. We see in our data that there is an intermediate concentration of RHamnoes for which part of the population shows the same fluoresce as the population with no Rhamnose added. Interesting for our own purposes because we cannot control the production of fimH like we thought we could.

Team:Harvard BioDesign/Description

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