Team:Waterloo/Experiments
Experiments & Protocols
- Seeds of Arabidopsis thaliana
- Agrobacterium tumefaciens strains such as C58C1
- Luria-Bertani (LB) media (broth and agar plate)
- Induction medium: 10.5 g/L K2HPO4, 1 g/L (NH4)2SO4, 0.5 g/L NaCitrate, 1 g/L glucose, 1 g/L fructose, 4 g/L glycerol, 1 mM MgSO4, 10 mM 2-(N-morpholino) methanesulfonic acid (MES); adjust pH to 5.6, autoclave before use
- Infiltration medium: 10 mM MgSO4, 10 mM MES; adjust pH to 5.6, autoclave before use
- Rifampicin: dissolve in methanol to make 25 mg/mL stock solution and store in -20 C freezer
- Tetracycline: dissolve in 70% ethanol to make 12.5 mg/mL stock solution and store in -20 C freezer
- Kanamycin: dissolve in water to make 50 mg/mL stock solution and store in -20 C freezer
- Acetosyringone (3,5-dimethoxy-4-hydroxyacetophenone, Aldrich): dissolve in 196.2 mg acetosyringone in 1 mL dimethylsulfoxide to make 1 M stock solution and store in -20 C freezer
- Dexamethasone (DEX, USB, Cleveland, OH): dissolve 78.4 mg DEX in 1 mL ethanol to make 200 mM stock solution
- 5-bromo-4-chloro-3-indolyl B-D-glucuronide (X-Glu, Sigma, St. Louis, MO): dissolve X-Glu in dimethylformamide to make 200 mM stock solution and store in -20 C freezer
- GUS staining solution: 50 mM sodium phosphate (pH 7.0), 1 mM EDTA, 2 mM X-Glu, 0.05% SDS, 0.1% sodium N-lauryl-sarcosine, 0.1% Triton-X-100
- Vacuum pump
Describe the experiments, research and protocols you used in your iGEM project.
What should this page contain?
- Protocols
- Experiments
- Documentation of the development of your project