Team:EPF Lausanne/Basic Part

EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits

OUR BEST NEW PART

PAM rich URS J23117Alt promoter

This promoter was made on the same template as PAM rich URS J23117 promoter [1], but was mutated to use different sgRNAs. It is also fused to a PAM rich upstream regulatory region (URS). Remember that dCas9 can bind only to PAM-flanked target sites.

EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits

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