Team:Bielefeld-CeBiTec/Notebook/PlasmidDisplay

Plasmid Repressor Interaction Assay

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Week 1 (4/20/2015-4/26/2015)

• Heat shock transformation of Biobricks needed for further assays was performed. Glycerol stocks were generated and the plasmids isolated according to the kits manual. The relevant Biobricks were:


• BBa_K525998: T7 promotor and RBS
• BBa_C0012: lacI repressor with LVA Tag
• BBa_R0010: lac Promotor
• BBa_K592008: lac Operator


• Furthermore a plasmid with a gene coding for a histagged lacI Protein was transformed, which was kindly provided by Stefan Hoffmann.

Week 2 (4/27/2015-5/3/2015)

• Purification of histagged lacI
• Test digest of transformed Biobricks: all clones as expected!
• Midi-preps of BBa_R0010 and BBa_K592008

Week 3 (5/4/2015-5/10/2015)

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Week 4 (5/11/2015-5/17/2015)

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Week 5 (5/18/2015-5/24/2015)

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Week 6 (5/25/2015-5/31/2015)

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Week 7 (6/1/2015-6/7/2015)

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Week 8 (6/8/2015-6/14/2015)

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Week 9 (6/15/2015-6/21/2015)

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Week 10 (6/22/2015-6/28/2015)

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Week 11 (6/29/2015-7/5/2015)

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