Team:Hong Kong-CUHK/Parts

An important aspect of the iGEM competition is the use and creation of standard biological parts.

Here is a table of summary of the parts that we constructed or have submitted to the registry

Name	Basic/Composite	Natural function of part	Usage	Copies
BBa_K1648000	Basic Part	flanking sequence of mamHIEJKLMN from Magnetosome Island (MAI)	Template for homologous recombination with mamAB operon	In pSB1C3 (submitted)
BBa_K1648001	Basic Part	flanking sequence of mamOPAQRBSTU from Magnetosome Island (MAI)	Template for homologous recombination with mamGC, mamXY, mms operons	Planing
BBa_K1648002	Composite part	Biobrick for homologous recombination with mamAB operon from Magnetosome Island (MAI)	Template for homologous recombination with mamAB operon(contains a promotor and terminator)	In pSB1A3 (stocked) In pSB1C3 (submitted)
BBa_K1648003	Composite part	Biobrick for homologous recombination with mamGC, mamXY, mms operons from Magnetosome Island (MAI)	Template for homologous recombination with mamGC, mamXY, mms operons(contains a promotor and terminator)	In pSB1C3 (submitted)
BBa_K1648004	Basic Part	Biobrick for mamC fuse protein on magnetosome membrane	Template for fuse protein of interest to magnetosome membrane	In pSB1C3 (submitted)
BBa_K1648005	Basic Part	Functional biobrick for GFP nanobody	Express GFP nanobody to conduct immunoprecipitation with GFP	In pSB1C3 (submitted)
BBa_K1648006	Composite part	Functional biobrick for mamC fuse protein on magnetosome membrane and GFP nanobody	fuse GFP nanobody to magnetosome membrane	In pSB1C3 (submitted)
BBa_K1648045	Basic	oprF from Azotobacter vinelandii	Allows non-specific transport from bacteria and extracellular environment	In pSB1C3 (submitted)
BBa_K1648046	Basic	Mutated oprF from Azotobacter vinelandii with five-point mutations	Give 5-fold transmission efficiency than original oprF	Planing
BBa_K1648047	Composite	oprF from Azotobacter vinelandii with RBS	Intermediate part for transcription of oprF	Planing
BBa_K1648048	Composite	oprF from Azotobacter vinelandii promoted by tetRpro	Constitutive production of oprF which allows non-specific transport from bacteria and extracellular environment	In pSB1A3 (submitted)
BBa_K1648049	Composite	Mutated oprF from Azotobacter vinelandii with five-point mutations promoted by tetRpro	Constitutive production of mutated oprF	In pSB1C3 (submitted)
BBa_K1648050	Basic	suspecting porin from Pseudomonas fluorescens with constitutive promoter	Expression of oprF for characterization	In pSB1C3 (submitted)
BBa_K1648051	Basic	Forming porin on Azotobacter vinelandii and templates for overexpressing hydrogenase in Azotobacter vinelandii	overexpress both porin and hydrogenase once transformed into Azotobacter vinelandii with homologous recombination	In pSB1C3 (submitted)