Part Documentation
- Part: K1681000
- Part Name: PL lac O-1 SCRIBE(kanR)ON
- Part Type: n/a
- Creator: UIUC_Illinois_2015
- Short Description: IPTG-inducible kanamycin resistance SCRIBE cassette
- Long Description:
SCRIBE(kanR)ON is a cassette coding for the RNA and proteins needed to restore kanamycin resistance by synthesizing multicopy single-stranded DNA (msDNA) for site-specific recombination. The part consists of the IPTG-inducible promoter PL lac O-1, a retron coding for an untranslated RNA molecule and the open reading frame for reverse transcriptase, a RBS, the open reading frame for beta recombinase, and a terminator. The reverse transcriptase uses the msDNA (msd) region of the RNA molecule as a template and the msd RNA (msr) region of the RNA molecule as a primer to synthesize the msDNA. The beta recombinase inserts the kanamycin resistance segment of the msDNA into the host’s genome during DNA replication as an Okazaki fragment.
- Design Considerations:
This part was designed to be used as a positive control for testing SCRIBE cassettes with different promoters and/ or custom DNA segments for site-specific recombination.
Part Table
<groupparts>iGEM015 Example</groupparts>
