Team:Lethbridge HS/Results
Results
What results did we get in the lab?
Results
Oxalic acid results:
Oxalic Acid Concentration (mg/mL) | OD528 |
---|---|
0.1 | 1.862 |
0.2 | 1.675 |
0.3 | 1.675 |
0.4 | 1.655 |
0.5 | 1.673 |
0.6 | 1.680 |
0.7 | 1.655 |
0.8 | 1.630 |
0.9 | 1.587 |
1.0 | 1.564 |
![](https://static.igem.org/mediawiki/2015/c/c9/LethHS2015_oxalicacidcurve.jpg)
Bee trials:
Treatment | Before | After |
---|---|---|
None | Somewhat lethargic. Drank when prompted. | Appeared tired, but otherwise unaffected. |
0.00% | Most lethargic, did not eat when offered | Wet and slow moving. |
0.05% | Very vigorous; ran around inside the tube. Fed. | Very energetic. Unaffected by treatment. |
0.2% | Very vigorous; ran around inside the tube. Fed. | Slightly less energetic than the 0.05% bee. |
The bees will be able to survive all of the oxalic acid that the E.coli can produce.
Mite trials:
Control | Oxalic Acid | RFP | |
---|---|---|---|
Parafilm Pouch | Mites dead | Mites dead | Mites dead |
Larvae 1 | Mites dead | Mites dead | Mites dead |
Larvae 2 | Mites dead | Mites dead | Mites dead |
The mites did not survive any of the trials.
Antibiotics testing:
Test (Antibiotics) | Result | After |
---|---|---|
AMP 1 | Positive | |
AMP 2 | Positive | Wet and slow moving. |
CAM 1 | Positive | |
CAM 2 | Positive | |
TET 1 | Positive | |
TET 2 | Positive | |
Control (water) | Positive |
The results we got for antibiotics testing show that biofilms can still be recultured even after being treated with antibiotics.
Testing with cleaners
Cleaner | Results | After |
---|---|---|
Bleach | - | |
Tilex | 3 | |
Mr. Clean | - | |
Fantastik | 1 | |
Truly | 2 | |
Windex | 1 | |
Lysol | - | |
GreenWorks | 1 |
![](https://static.igem.org/mediawiki/2015/5/55/LetHS_2015_PDPConstructAcidProduction.png)
![](https://static.igem.org/mediawiki/2015/a/af/LethHS2015_5.png)
![](https://static.igem.org/mediawiki/2015/1/1c/LethHS2015_6.jpg)
![](https://static.igem.org/mediawiki/2015/4/48/LethHS2015_7.png)
![](https://static.igem.org/mediawiki/2015/c/c3/LethHS2015_8.jpg)
![](https://static.igem.org/mediawiki/2015/f/fd/LethHS201510.png)
![](https://static.igem.org/mediawiki/2015/d/d3/LethHS201511.png)