Team:Michigan/Notebook/July

July

7/02/2015
-Tested Liu Lab plasmids and our switches (G/H)
In Vitro Translation kit salt con:
[Mg] = 8-12nM
[K] = 120nM

6/15/2015
-Made ampicillin and chloramphenicol plates

6/16/2015
-Plasmid transformation of plasmids we obtained using 1ul of DNA and 50uls of NEB competent cells

6/17/2015
-Made cultures from colonies of transformation

6/18/2015
-Mini-prep of plasmids from cultures
-Sent for sequencing with double primers

6/22/2015
-Miniprep of Liu lab plasmids. Stored in yellow rack in the freezer
-Sequence verified

6/28/2015
-Plasmid transformations of both switches from synthesis order (G flip and H flip)
-Diluted synthesis with 50uls of ultra pure water, so we should have a concentration of 80ug/ul
-pIDTBlue vector from IDT has amp resistance
-NOTE: one of the unused Amp plate had contamination (undesired colony), once we get the plasmid transformation colonies, we should transfer them to new Amp plates. Need to get new Amp

6/29/2015
-pick colonies from plasmid transformations to miniprep
-NOTE: New Amp is on the fridge

6/30/2015
-Miniprep G and H switch flip

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