Team:SVCE Chennai/Notebook
Notebook
APRIL 2015
- Recruitment of team members
- Setting up of lab with necessary equipments and chemicals
- Brainstorming
MAY- MID JUNE 2015
- Brainstorming
- University exams hence no lab work
MID JUNE- JULY 2015
- lab practice sessions
- confirmation of project title
AUGUST 2015
Week 1
- Submitted safety forms and also regarding our project forms.
- Preparation of LB broth and LB Agar and plates were poured.
- Stab culture for FtsZ was obtained from iGEM and was streaked on chloramphenicol LB plates.
- Abstract and title of our project was uploaded.
- Prepared chemicals and buffers for plasmid isolation.
- FtsZ was innoculated in LB broth.
- Pure culture of Pseudomonas aeruginosa and BL21 strain was obtained.
- Sequences for gBlocks were constructed for ordering.
Week 2:
- Plasmid isolation was performed for protocol optimisation.
- Result was negative and so the isolation was performed again.
- Elution of DNA was done but results were not satisfactory.
- Media (SOB and SOC) were prepared for competent cell preparation.
- Positive results were obtained for elution of DNA and protocol was optimised.
- Innoculated BL 21 in SOB.
- Presentation and questionnaire based on our project was prepared to educate the students in different colleges.
- Logo designing was initiated.
Week 3:
- Growth curve for Pseudomonas aeruginosa was done and results were analysed.
- Growth curve for BL 21 was done.
- Plasmid isolation of pET24a was performed.
- Competent cell of BL 21 was made and protocol was optimised.
- Seed stock for BL 21 competent cell was made.
- Transformation was performed.
- Primers required for our project were designed and ordered.
- For Human Practise questionnaire for hospitals were prepared.
- Presentation was done in Arulmigu Meenakshi Amman College and in Prathyusha Institute of Technology and Management.
- Dr. Kannan from Saveetha Medical College was interviewed regarding antibiotic resistance.
- Logo design was reviewed and changes were made.
- Approached various funding agencies.
- Collaboration with Uppsala was confirmed.
Week 4:
- BL21 was subcultured.
- Transformation efficiency of pET 24a in E coli BL21 competent cells was calculated.
- Chloramphenicol plates were prepared for pSB1C3 amplification.
- Methicillin Resistant Staphylococcus aureus were obtained.
- Final safety forms were filled.
- First step was taken for wiki page and its theme was discussed.
- Responses for various questionnaire was collected and a statistical analysis was carried out.
- Results were presented to the faculty members of our department.
- Three doctors from reputed medical institutions were interviewed regarding the development of antibiotic resistance and formation of biofilms in hospital environments.
SEPTEMBER 2015
Week 1:
- gBlocks of ftsZ was obtained.
- PCR amplification of the ftsZ was done.
- Results were found to be negative.
- Hence they were troubleshooted.
- Biochemical tests were performed for Staphylococcus and Pseudomonas. A presentation was done during the national level symposium called OMICS and created an awareness among various other college students.
Week 2:
- gBlocks of thuricin was obtained.
- PCR amplification of the gene was done.
- This amplified gene was cloned into pSB1C3.
- Growth curve for Staphylococcus aureus was done.
- Human practices team presented in Madha Engineering College and SBOA.
Week 3:
- Got bactofencin gBlocks and PCR amplification was done.
- Then cloning was done in pSB1C3.
- Cloned Thuricin and Bactofencin were submitted as parts.
- Public interview regarding antibiotic resistance was taken.
- Descriptions were uploaded in wiki page.