Team:Paris Bettencourt/Notebook/VitaminA
Jul. 14th
Goal
Extract the integrative plasmid HO-Poly-KanMX4-HO from the E.Coli provided by AddGene (Accession number #51662).Procedure
- Liquid culture overnight in LB.
- Centrifuge the tubes for 1 minute with 11000 rpm.
- Follow the standard protocol of Thermo Scientific miniprep kit (is it? I followed Ihab's protocol here: https://docs.google.com/document/d/1QHBHcN8RY0c-oEY8X5kAqPrKYODTBm7K8eU_lY_yw-o/edit, we should compare them)
- Measure concentration with Nanodrop
- Label the centrifuge tube and store it in the -20 freezer. (g15.35)
Results
Final DNA concentrations of the 4 tubes of miniprep, measured with Nanodrop:- tube HO pl. 1 = 431.1 ng/uL
- tube HO pl. 2 = 313.6 ng/uL
- tube HO pl. 3 = 366.4 ng/uL
- tube HO pl. 4 = 261.7 ng/uL
- Made the project, it is awesome!
Jul. 24th