Team:Paris Bettencourt/Notebook/VitaminA

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Jul. 14th

Goal

Extract the integrative plasmid HO-Poly-KanMX4-HO from the E.Coli provided by AddGene (Accession number #51662).

Procedure

• Liquid culture overnight in LB.
• Centrifuge the tubes for 1 minute with 11000 rpm.
• Follow the standard protocol of Thermo Scientific miniprep kit (is it? I followed Ihab's protocol here: https://docs.google.com/document/d/1QHBHcN8RY0c-oEY8X5kAqPrKYODTBm7K8eU_lY_yw-o/edit, we should compare them)
• Measure concentration with Nanodrop
• Label the centrifuge tube and store it in the -20 freezer. (g15.35)

Results

Final DNA concentrations of the 4 tubes of miniprep, measured with Nanodrop:

• tube HO pl. 1 = 431.1 ng/uL
• tube HO pl. 2 = 313.6 ng/uL
• tube HO pl. 3 = 366.4 ng/uL
• tube HO pl. 4 = 261.7 ng/uL


Jul. 24th
• Made the project, it is awesome!