PROTOCOLS Medium LB medium(100mL) Tryptone ------ 1.0g Yeast Extract ------ 0.5g NaCl ------ 1.0g H2O ------ 100mL agar ------ 1.5g (in case of agar medium) SOC medium(100mL) Tryptone ------ 2.0g Yeast Extract ------ 0.5g 1M NaCl ------ 1.0mL 1M KCl ------ 0.25mL H2O