Team:Bielefeld-CeBiTec/Primers
Primers
Name | Sequence (5´ → 3´) |
---|---|
Standard primers | |
SB-prep-2Ea | ATGAATTCCAGAAATCATCCTTAGCG |
SB-prep-3P-1 | GCCGCTGCAGTCCGGCAAAAAA |
VF | CCACCTGACGTCTAAGAA |
VR | GTATTACCGCCTTTGAGT |
Arsenic biosensor | |
Ars_1_rev | tatgacttaacgaatgtgtaagtgcagaggCGGATAAAACACATCTGAAAATTC |
Ars_2_fwd | cctctgcacttacacattcgttaagtcataGGTACTAGTAGCGGCC |
Ars_3_fwd | cattcgttaagtcatatatgtttttgacttGGTACTAGTAGCGGCC |
Ars_3_rev | tcaaaaacatatatgacttaacgaatgtgtCGGATAAAACACATCTGAAAATTC |
arsRhis_arsR_fwd | tatagggaaagaggagaaatactagagATGTCATTTCTGTTACCCATC |
arsRhis_arsR2_r | ctagtattagtggtgatggtgatgatgACTGCAAATGTTCTTACTG |
arsRhis_Trp_fwd | CATCATCACCATCACCAC |
arsRhis_Trp_rev | CTCTAGTATTTCTCCTCTTTCC |
UTR_arsO_rev | ccttcttttttttttttaaagttaaacaaaattatTAAGTCAAAAACATATATGACTTAACG |
UTR_noT7_rev | ccttcttttttttttttaaagttaaacaaaattattCTCTAGAAGCGGCCGCGA |
Date rape drugs | |
BlcR_fwd | ATGGGCCAAAGGGGCCAAGT |
BlcR_rev | CTAGTCTTTCTGCGATCGGTACCC |
pSB1C3_BlcR_fwd | cttgggtaccgatcgcagaaagactagTACTAGTAGCGGCCGCTGCAG |
psB_BlcRfwd_new" | CTTGGGTACCGATCGCAGAAAGACTAATACTAGTAGCGGCCGCTGCAG |
pSB1C3_BlcR_rev | ttgacacacttggcccctttggcccatctagtaTTTCTCCTCTTTCTCTAGTAGCTAGCACAATAC |
BlcC_rev | TTACGCGTAAAATTCGGGAGCCTTC |
BlcC_rev_new | TTAGTCTTTCTGCGATCGGTACCC |
bclR-rv" | GTCTTTCTGCGATCGGTACCC |
blcR-CBD-fw | AAGCTTGGGTACCGATCGCAGAAAGACCCCGGCGCCAATCCGCCCGGT |
pro_Blc_fwd | ATGCACCTTCCTTGACACACT |
pro_Blc_rev | CATCGACCTCTCCCGCATTAG |
K516030proB_rev | gggccaagtgtgtcaaggaaggtgcatCTCTAGAAGCGGCCGCGAATTCC |
K516030proB_fwd | tgaactctaatgcgggagaggtcgATGGCTTCCTCCGAAGACGTTAT |
BlcB_mut_fwd | CCTTTCGAGTTCCGCACCGTTCCCT |
BlcB_mut_rev" | AGGGAACGGTGCGGAACTCGAAAGG |
BlcR-L-sfGFP-rv | TCGCCTTTACGCATGCCGGTGTCTTTCTGCGATCGGTACCC |
BlcR-T7-RBS-fw | TAGAGAAAGAGGAGAAATACTAGATGGGCCAAAG |
K60_BlcPshort_r | GAGTGAACTATGGCTCTAGTAGCTAGCACAATACC |
K60_BlcPshort_f" | GCTAGCTACTAGAGCCATAGTTCACTCTAATGATTC |
bclR-rv | GTCTTTCTGCGATCGGTACCC |
pSB1C3blcOnewfw | AAGAAGGCTCCCGAATTTTACGCGTAATACTAGTAGCGGCCGCTGCAG |
gBlocks | |
GABA_B_pSB1C3_f | ataagaaaataccgacagggggatattcacTACTAGTAGCGGCCGCTGC |
GABA_B_pSB1C3_r | cataaaaaaatcccagtcacaggtgattgaCTCTAGAAGCGGCCGCGA |
GABA_R_pSB1C3_f | tagttttcaaccctttcagcggaggagccatcgTACTAGTAGCGGCCGCTGC |
GABA_R_pSB1C3_r | ctgtggattaacacaccacctactttttaaCTCTAGAAGCGGCCGCGA |
Trp_sy_pSB1C3_f | aaagaagttcatcatcaccatcaccactaaTACTAGTAGCGGCCGCTGC |
Trp_sy_pSB1C3_r | tctcctctttccctatagtgagtcgtattaCTCTAGAAGCGGCCGCGA |
Split primers | |
Cm_fwd | CGGCATCAGCACCTTGTC |
Cm_rev | TATACGCAAGGCGACAAG |