Team:Bielefeld-CeBiTec/Primers
Primers
| Name | Sequence (5´ → 3´) |
|---|---|
| Standard primers | |
| SB-prep-2Ea | ATGAATTCCAGAAATCATCCTTAGCG |
| SB-prep-3P-1 | GCCGCTGCAGTCCGGCAAAAAA |
| VF | CCACCTGACGTCTAAGAA |
| VR | GTATTACCGCCTTTGAGT |
| Arsenic biosensor | |
| Ars_1_rev | tatgacttaacgaatgtgtaagtgcagaggCGGATAAAACACATCTGAAAATTC |
| Ars_2_fwd | cctctgcacttacacattcgttaagtcataGGTACTAGTAGCGGCC |
| Ars_3_fwd | cattcgttaagtcatatatgtttttgacttGGTACTAGTAGCGGCC |
| Ars_3_rev | tcaaaaacatatatgacttaacgaatgtgtCGGATAAAACACATCTGAAAATTC |
| arsRhis_arsR_fwd | tatagggaaagaggagaaatactagagATGTCATTTCTGTTACCCATC |
| arsRhis_arsR2_r | ctagtattagtggtgatggtgatgatgACTGCAAATGTTCTTACTG |
| arsRhis_Trp_fwd | CATCATCACCATCACCAC |
| arsRhis_Trp_rev | CTCTAGTATTTCTCCTCTTTCC |
| UTR_arsO_rev | ccttcttttttttttttaaagttaaacaaaattatTAAGTCAAAAACATATATGACTTAACG |
| UTR_noT7_rev | ccttcttttttttttttaaagttaaacaaaattattCTCTAGAAGCGGCCGCGA |
| Date rape drugs | |
| BlcR_fwd | ATGGGCCAAAGGGGCCAAGT |
| BlcR_rev | CTAGTCTTTCTGCGATCGGTACCC |
| pSB1C3_BlcR_fwd | cttgggtaccgatcgcagaaagactagTACTAGTAGCGGCCGCTGCAG |
| psB_BlcRfwd_new" | CTTGGGTACCGATCGCAGAAAGACTAATACTAGTAGCGGCCGCTGCAG |
| pSB1C3_BlcR_rev | ttgacacacttggcccctttggcccatctagtaTTTCTCCTCTTTCTCTAGTAGCTAGCACAATAC |
| BlcC_rev | TTACGCGTAAAATTCGGGAGCCTTC |
| BlcC_rev_new | TTAGTCTTTCTGCGATCGGTACCC |
| bclR-rv" | GTCTTTCTGCGATCGGTACCC |
| blcR-CBD-fw | AAGCTTGGGTACCGATCGCAGAAAGACCCCGGCGCCAATCCGCCCGGT |
| pro_Blc_fwd | ATGCACCTTCCTTGACACACT |
| pro_Blc_rev | CATCGACCTCTCCCGCATTAG |
| K516030proB_rev | gggccaagtgtgtcaaggaaggtgcatCTCTAGAAGCGGCCGCGAATTCC |
| K516030proB_fwd | tgaactctaatgcgggagaggtcgATGGCTTCCTCCGAAGACGTTAT |
| BlcB_mut_fwd | CCTTTCGAGTTCCGCACCGTTCCCT |
| BlcB_mut_rev" | AGGGAACGGTGCGGAACTCGAAAGG |
| BlcR-L-sfGFP-rv | TCGCCTTTACGCATGCCGGTGTCTTTCTGCGATCGGTACCC |
| BlcR-T7-RBS-fw | TAGAGAAAGAGGAGAAATACTAGATGGGCCAAAG |
| K60_BlcPshort_r | GAGTGAACTATGGCTCTAGTAGCTAGCACAATACC |
| K60_BlcPshort_f" | GCTAGCTACTAGAGCCATAGTTCACTCTAATGATTC |
| bclR-rv | GTCTTTCTGCGATCGGTACCC |
| pSB1C3blcOnewfw | AAGAAGGCTCCCGAATTTTACGCGTAATACTAGTAGCGGCCGCTGCAG |
| gBlocks | |
| GABA_B_pSB1C3_f | ataagaaaataccgacagggggatattcacTACTAGTAGCGGCCGCTGC |
| GABA_B_pSB1C3_r | cataaaaaaatcccagtcacaggtgattgaCTCTAGAAGCGGCCGCGA |
| GABA_R_pSB1C3_f | tagttttcaaccctttcagcggaggagccatcgTACTAGTAGCGGCCGCTGC |
| GABA_R_pSB1C3_r | ctgtggattaacacaccacctactttttaaCTCTAGAAGCGGCCGCGA |
| Trp_sy_pSB1C3_f | aaagaagttcatcatcaccatcaccactaaTACTAGTAGCGGCCGCTGC |
| Trp_sy_pSB1C3_r | tctcctctttccctatagtgagtcgtattaCTCTAGAAGCGGCCGCGA |
| Split primers | |
| Cm_fwd | CGGCATCAGCACCTTGTC |
| Cm_rev | TATACGCAAGGCGACAAG |
The gene-specific part of the primer is written in uppercase letters, while lowercase letters denote an overhang.