Team:Bielefeld-CeBiTec/DataPage

iGEM Bielefeld 2015


Data Page

An Overview of our favourite BioBricks

Data for CFPS

BioBrickDescription
BBa_K1758100 Translation enhancing 5'-untranslated region (UTR) containing g10-L RBS
BBa_K1758101 5'-untranslated region (UTR) containing g10-L RBS in front of sfGFP coding sequence
BBa_K1758102 T7 promoter in front of 5'-untranslated region (UTR) followed by sfGFP coding sequence
BBa_K1758105 T7 promoter followed by mRFP generator
BBa_K1758106 T7 promoter followed by 5'-untranslated region (UTR) and mRFP generator
BBa_K1758107 T7 promoter and RBS followed by SYFP2 coding sequence
BBa_K1758120 Coding sequence of RNase E regulating protein RraA from E. coli
BBa_K1758122 Coding sequence of RNase E regulating protein RraA under control of T7 promoter, with N-terminal His-tag and TEV cleavage site

Data for PRIA

BioBrickDescription
BBa_K1758201Lac Repressor with His-Tag under control of T7 promoter and a strong RBS
BBa_K1758202Lac repressor fused with superfolding Green Fluorescence Protein (sfGFP) with His-Tag under control of T7 promoter and a strong RBS
BBa_K1758203 Arsenic repressor fused with superfolding Green Fluorescence Protein (sfGFP) with His-Tag under control of T7 promoter and a strong RBS
BBa_K1758204 Blc repressor fused with superfolding Green Fluorescence Protein (sfGFP) with His-Tag under control of T7 promoter and a strong RBS

Data for Heavy metals

BioBrickDescription
BBa_K1758310Chromium Repressor under control of constitutive Promoter and strong RBS
BBa_K1758311 Chromium responsive promoter with RFP
BBa_K1758312 Chromium responsive promoter with UTR+RBS and sfGFP
BBa_K1758313 Chromium repressor under control of constitutive promoter and strong RBS, chromium responsive promoter, UTR+RBS and sfGFP
BBa_K1758314 Chromium responsive promoter with T7-promoter and UTR-sfGFP
BBa_K1758320 Copper activator under control constitutive promoter and strong RBS
BBa_K1758321 Copper responsive promoter with RFP
BBa_K1758322 Copper activator under control constitutive promoter and strong RBS and Copper responsive promoter with RFP
BBa_K1758323 Copper responsive promoter with UTR-sfGFP
BBa_K1758324 Copper activator under control constitutive promoter and strong RBS and Copper responsive promoter with UTR-sfGFP
BBa_K1758325 Copper responsive promoter with T7-promoter and UTR-sfGFP
BBa_K1758330 Lead repressor under control of constitutive promot er and strong RBS
BBa_K1758331 Lead responsive promoter with RFP
BBa_K1758332 Lead responsive promoter with UTR-sfGFP
BBa_K1758333 Lead repressor under control of constitutive promoter and strong RBS and lead responsive promoter with UTR-sfGFP
BBa_K1758334 Lead responsive promoter with T7-promoter and UTR-sfGFP
BBa_K1758340 Mercury repressor under control of constitutive promoter and strong RBS
BBa_K1758342 Mercury responsive promoter with UTR-sfGFP
BBa_K1758343 Mercury activator under control of constitutive promoter and strong RBS and mercury responsive promoter with UTR-sfGFP
BBa_K1758344 Mercury responsive promoter with T7-promoter and UTR-sfGFP
BBa_K1758350 Nickel repressor under control of constitutive promoter and strong RBS
BBa_K1758351 Nickel responsive promoter with RFP
BBa_K1758352 Nickel responsive promoter with UTR-sfGFP
BBa_K1758353 Nickel activator under control of constitutive promoter and strong RBS and Nickel responsive promoter with UTR-sfGFP
BBa_K1758354 Nickel responsive promoter with T7-promoter and UTR-sfGFP

Data for Date Rape Drugs

BioBrickDescription
BBa_K1758370 Repressor of blcABC operon, BlcR, under control of constitutive promoter with RBS
BBa_K1758375 Promoter of blcABC operon followed by mRFP cds and terminator
BBa_K1758376 T7 promoter in front of Blc operator sequence followed by 5'-UTR and sfGFP
BBa_K1758377 T7 promoter in front of Blc operator sequence followed by 5'-UTR and sfGFP, combined with repressor BlcR under control of constitutive promoter