Team:Hong Kong-CUHK/wetlabjournal
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March
1. Learning Lab techniques and protocols
2. Brainstorming ideas
3. Researches
April
1. Project decided
2. Preparation of special medium and plate for A. vinelandii
3. Study the growth curve characteristics of A. vinelandii
4. Preparation of Competent Cells of dh5α and BL21, aliquot the competent cells, stored in liquid nitrogen and then -80C refrigerator.
5. Make plate with antibiotics -Ampicillin, kanamycin and chloramphenicol.
6. Make LB brooth
May
1. Purchase for primers for the magnetosome project.
2. Primer dilution of the all arrived primers
3. Amplification by using PCR, following by run gel of A. vinelandii ___ gene.
4. Make plate with antibiotics -Ampicillin, kanamycin and chloramphenicol
5. Make LB brooth
June
1. Amplification by using PCR, following by run gel of A. vinelandii ___ gene.
2. Restriction of PCR product ______ , ligase into C backbone and transform into dh5α
3. Make plate with antibiotics -Ampicillin, kanamycin and chloramphenicol
4. Make LB brooth
July
1. Amplification by using PCR, following by run gel of A. vinelandii ___ gene.
2. Restriction of PCR product ______ , ligase into C backbone and transform into dh5α
3. Preparation of Competent Cells of dh5α and BL21, aliquot the competent cells, stored in liquid nitrogen and then -80C refrigerator.
4. Make plate with antibiotics -Ampicillin, kanamycin and chloramphenicol
5. Make LB brooth
August
1. Preparation of Competent Cells of A. vinelandii
2. Amplification by using PCR, following by run gel of A. vinelandii ___ gene.
3. Overlapping PCR of A. vinelandii nifB gene, A. vinelandii nifQ gene and A. vinelandii FdxA gene
4. Restriction of PCR product ______ , ligase into C backbone and transform into dh5α.
5. Make plate with antibiotics -Ampicillin, kanamycin and chloramphenicol
6. Make LB brooth
September
1. Amplification by using PCR, following by run gel of A. vinelandii ___ gene.
2. Restriction of PCR product ______ , ligase into C backbone and transform into dh5α
3. Make plate with antibiotics -Ampicillin, kanamycin and chloramphenicol
4. Make LB brooth
5. Submission of all biobricks to the registry
