Difference between revisions of "Team:UChicago"

 
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{{UChicago/Header}}
 
{{UChicago/Header}}
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  Name="List Table 6 Colorful Accent 1"/>
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  Name="List Table 6 Colorful Accent 3"/>
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  Name="List Table 6 Colorful Accent 6"/>
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  <w:LsdException Locked="false" Priority="52"
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  Name="List Table 7 Colorful Accent 6"/>
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+
 
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Line 657: Line 10:
 
  @font-face
 
  @font-face
 
{font-family:"Cambria Math";
 
{font-family:"Cambria Math";
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@font-face
 
@font-face
 
{font-family:Calibri;
 
{font-family:Calibri;
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@font-face
 
@font-face
 
{font-family:Georgia;
 
{font-family:Georgia;
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font-size:12.0pt;
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Line 711: Line 37:
 
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a:link, span.MsoHyperlink
 
a:link, span.MsoHyperlink
{mso-style-priority:99;
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<body lang=EN-US link="#0563C1" vlink="#954F72">
  
 
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<h1 align=center style='text-align:center'><span style='font-size:26.0pt;
+
<h1 align=center style='text-align:center'><b><span style='font-size:32.0pt;
line-height:107%;font-family:"Calibri",sans-serif;mso-ascii-theme-font:minor-latin;
+
line-height:107%;font-family:"Calibri",sans-serif;color:#C00000'>Transplanting
mso-fareast-font-family:"Times New Roman";mso-hansi-theme-font:minor-latin;
+
KaiABC: Reduce your jet lag with this one weird trick!</span></b></h1>
mso-bidi-font-family:"Courier New";color:#C00000'>Transplanting <span
+
class=SpellE>KaiABC</span>: Reduce your jet lag with this one weird trick!</span><span
+
style='font-size:26.0pt;line-height:107%;font-family:"Calibri",sans-serif;
+
mso-ascii-theme-font:minor-latin;mso-fareast-font-family:"Times New Roman";
+
mso-hansi-theme-font:minor-latin;mso-bidi-font-family:"Courier New";color:red'><o:p></o:p></span></h1>
+
  
 
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normal'><span style='font-size:16.0pt;font-family:"Georgia",serif'>&nbsp;</span></p>
"Times New Roman"'><o:p>&nbsp;</o:p></span></p>
+
  
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</v:shapetype><v:shape id="Picture_x0020_1" o:spid="_x0000_i1028" type="#_x0000_t75"
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</v:shape><![endif]--><![if !vml]><img width=469 height=313
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src="/wiki/images/1/15/UChicago2015Home_filesimage002.jpg"
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<v:imagedata src="/wiki/images/c/c3/UChicago2015Home_filesimage003.jpg" o:title="ANd9GcTDiy2Lx1WnrBiecDru0X-iK26C0zx9oPrvT2-FAaq9cT6oF624dA"/>
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</v:shape><![endif]--><![if !vml]><img width=504 height=314
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v:shapes="Picture_x0020_2"><![endif]><!--[if gte vml 1]><v:shape id="Picture_x0020_3"
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o:spid="_x0000_i1026" type="#_x0000_t75" alt="http://static.tvtropes.org/pmwiki/pub/images/Dino_F-14s.png"
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style='width:315pt;height:235.5pt;visibility:visible;mso-wrap-style:square'>
+
<v:imagedata src="http://static.tvtropes.org/pmwiki/pub/images/Dino_F-14s.png"/>
+
</v:shape><![endif]--><![if !vml]><img width=420 height=314
+
src="/wiki/images/3/3f/UChicago2015Home_filesimage006.jpg"
+
alt="http://amfblog.s3.amazonaws.com/wp-content/uploads/2015/08/02003640/sleepsleep.gif"v:shapes="Picture_x0020_3"><![endif]></span><b><span
+
style='font-size:16.0pt;font-family:"Georgia",serif;mso-fareast-font-family:
+
"Times New Roman";mso-bidi-font-family:Arial;color:black'><o:p></o:p></span></b></p>
+
  
<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
+
<p class=MsoNormal align=center style='margin-bottom:0in;margin-bottom:.0001pt;
normal'><i style='mso-bidi-font-style:normal'><span style='font-family:"Arial",sans-serif;
+
text-align:center;line-height:normal'><i><span style='font-family:"Arial",sans-serif;
mso-fareast-font-family:"Times New Roman";color:black;mso-bidi-font-weight:
+
color:black'>Do you see these images in your jet-lag-drunken nightmares? I know
bold'>Do you see these images in your jet-lag-drunken nightmares? I know I do.<o:p></o:p></span></i></p>
+
I do.</span></i></p>
  
 
<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
 
<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
normal;tab-stops:406.75pt'><b><span style='font-size:16.0pt;font-family:"Georgia",serif;
+
normal'><b><span style='font-size:16.0pt;font-family:"Georgia",serif;
mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:Arial;
+
color:black'>                                                                                                            </span></b></p>
color:black'><span style='mso-tab-count:1'>                                                                                                            </span><o:p></o:p></span></b></p>
+
  
 
<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
 
<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
 
normal'><b><span style='font-size:16.0pt;font-family:"Georgia",serif;
 
normal'><b><span style='font-size:16.0pt;font-family:"Georgia",serif;
mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:Arial;
 
 
color:black'>Have you ever felt tired as a weary traveler after a whole day of
 
color:black'>Have you ever felt tired as a weary traveler after a whole day of
flying in an unfamiliar city? So dogegone tired that you would do anything just
+
flying in an unfamiliar city? So doggone tired that you would do anything just
 
to feel refreshed anything? Then just click </span></b><a
 
to feel refreshed anything? Then just click </span></b><a
 
href="https://2015.igem.org/Team:UChicago/Description"><b><span
 
href="https://2015.igem.org/Team:UChicago/Description"><b><span
style='font-size:16.0pt;font-family:"Georgia",serif;mso-fareast-font-family:
+
style='font-size:16.0pt;font-family:"Georgia",serif'>here</span></b></a><b><span
"Times New Roman";mso-bidi-font-family:Arial'>here</span></b></a><b><span
+
style='font-size:16.0pt;font-family:"Georgia",serif;color:black'> to buy the
style='font-size:16.0pt;font-family:"Georgia",serif;mso-fareast-font-family:
+
ultimate cure to jet lag! Or just give us your credit card information </span></b><a
"Times New Roman";mso-bidi-font-family:Arial;color:black'> to buy the ultimate
+
cure to jet lag! Or just give us your credit card information </span></b><a
+
 
href="https://2015.igem.org/Team:UChicago/Description"><b><span
 
href="https://2015.igem.org/Team:UChicago/Description"><b><span
style='font-size:16.0pt;font-family:"Georgia",serif;mso-fareast-font-family:
+
style='font-size:16.0pt;font-family:"Georgia",serif'>here</span></b></a><b><span
"Times New Roman";mso-bidi-font-family:Arial'>here</span></b></a><b><span
+
style='font-size:16.0pt;font-family:"Georgia",serif;color:black'> and we’ll
style='font-size:16.0pt;font-family:"Georgia",serif;mso-fareast-font-family:
+
ship it to you pronto!</span></b></p>
"Times New Roman";mso-bidi-font-family:Arial;color:black'> and we’ll ship it to
+
you pronto!<o:p></o:p></span></b></p>
+
  
 
<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
 
<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
 
normal'><b><span style='font-size:16.0pt;font-family:"Georgia",serif;
 
normal'><b><span style='font-size:16.0pt;font-family:"Georgia",serif;
mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:Arial;
+
color:black'>&nbsp;</span></b></p>
color:black'><o:p>&nbsp;</o:p></span></b></p>
+
  
 
<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
 
<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
 
normal'><b><span style='font-size:16.0pt;font-family:"Georgia",serif;
 
normal'><b><span style='font-size:16.0pt;font-family:"Georgia",serif;
mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:Arial;
+
color:black'>…Just kidding! We’re not trying to scam you here, we’re the
color:black'>…Just kidding! We’re not trying to scam you here, we’re the <span
+
UChicago 2015 iGEM team and we’re trying to create bacteria that can <u>reduce
class=SpellE>UChicago</span> 2015 <span class=SpellE>iGEM</span> team and we’re
+
or cure your jet lag</u> (as well as sleeping disorders).</span></b></p>
trying to create bacteria that can <u>reduce or cure your jet lag</u> (as well
+
as sleeping disorders).<o:p></o:p></span></b></p>
+
  
 
<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
 
<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
 
normal'><b><span style='font-size:16.0pt;font-family:"Georgia",serif;
 
normal'><b><span style='font-size:16.0pt;font-family:"Georgia",serif;
mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:Arial;
+
color:black'>&nbsp;</span></b></p>
color:black'><o:p>&nbsp;</o:p></span></b></p>
+
  
 
<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
 
<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
normal'><span style='font-size:16.0pt;font-family:"Garamond",serif;mso-fareast-font-family:
+
normal'><span style='font-size:16.0pt;font-family:"Garamond",serif;color:black'>We
"Times New Roman";mso-bidi-font-family:Arial;color:black'>We actually have
+
actually have science to back us up! Currently, there exist no working 24-hour
science to back us up! Currently, there exist no working 24-hour oscillator <span
+
oscillator BioBricks. We are addressing this problem by transplanting the
class=SpellE>BioBricks</span>. We are addressing this problem by transplanting
+
circadian rhythm-generating KaiABC system from cyanobacteria into E. coli and
the circadian rhythm-generating <span class=SpellE>KaiABC</span> system from
+
optimizing its function by controlling protein stoichiometry and adding
cyanobacteria into E. coli and optimizing its function by controlling protein
+
accessory proteins. Such a BioBrick would be important for any application in
stoichiometry and adding accessory proteins. Such a <span class=SpellE>BioBrick</span>
+
which gene expression needs to be regulated on a 24-hour schedule. For example,
would be important for any application in which gene expression needs to be
+
it is often important to release a drug at a specific time of the day. As a
regulated on a 24-hour schedule. For example, it is often important to release
+
proof-of-concept application, we implemented this clock as a potential solution
a drug at a specific time of the day. As a proof-of-concept application, we
+
to jet lag and sleep disorders by engineering a strain of E. coli that
implemented this clock as a potential solution to jet lag and sleep disorders
+
facilitates the production of a melatonin precursor in the gut on a 24-hour
by engineering a strain of E. coli that facilitates the production of a
+
cycle. Thus, our project hopes to demonstrate the application of a robust
melatonin precursor in the gut on a 24-hour cycle. Thus, our project hopes to
+
KaiABC-based clock in timed drug dosage, as well as introduces an oscillator
demonstrate the application of a robust <span class=SpellE>KaiABC</span>-based
+
BioBrick into the synthetic biology community. </span></p>
clock in timed drug dosage, as well as introduces an oscillator <span
+
class=SpellE>BioBrick</span> into the synthetic biology community. <o:p></o:p></span></p>
+
  
 
<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
 
<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
normal'><span style='font-size:16.0pt;font-family:"Georgia",serif;mso-fareast-font-family:
+
normal'><span style='font-size:16.0pt;font-family:"Georgia",serif;color:black'>&nbsp;</span></p>
"Times New Roman";mso-bidi-font-family:Arial;color:black'><o:p>&nbsp;</o:p></span></p>
+
  
 
<p class=MsoNormal align=center style='margin-bottom:0in;margin-bottom:.0001pt;
 
<p class=MsoNormal align=center style='margin-bottom:0in;margin-bottom:.0001pt;
text-align:center;line-height:normal'><span style='mso-no-proof:yes'><!--[if gte vml 1]><v:shape
+
text-align:center;line-height:normal'><img border=0 width=662 height=832
id="Picture_x0020_4" o:spid="_x0000_i1025" type="#_x0000_t75" style='width:496.5pt;
+
id="Picture 4" src="/wiki/images/c/ca/UChicago2015Home_filesimage004.png"></p>
height:624pt;visibility:visible;mso-wrap-style:square'>
+
<v:imagedata src="/wiki/images/2/28/UChicago2015Home_filesimage007.png" o:title=""/>
+
</v:shape><![endif]--><![if !vml]><img border=0 width=662 height=832
+
src="/wiki/images/2/28/UChicago2015Home_filesimage007.png" v:shapes="Picture_x0020_4"><![endif]></span><span
+
style='font-size:16.0pt;font-family:"Georgia",serif;mso-fareast-font-family:
+
"Times New Roman"'><o:p></o:p></span></p>
+
  
 
<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
 
<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
normal'><i style='mso-bidi-font-style:normal'><span style='font-family:"Arial",sans-serif;
+
normal'><i><span style='font-family:"Arial",sans-serif;color:black'>Figure 1:
mso-fareast-font-family:"Times New Roman";color:black;mso-bidi-font-weight:
+
A) The first leg of our project focuses on reconstituting the KaiABC oscillator
bold'>Figure 1: A) The first leg of our project focuses on reconstituting the <span
+
system. B) The second leg of our journey (see what we did there?) focuses on
class=SpellE>KaiABC</span> oscillator system. B) The second leg of our journey
+
creating a readout system that can process KaiC phosphorylation state into
(see what we did there?) focuses on creating a readout system that can process <span
+
actual promoter state. Not depicted is the third part of our project, which
class=SpellE>KaiC</span> phosphorylation state into actual promoter state. Not
+
aims to secrete AANAT, an enzyme that can produce a melatonin precursor.</span></i></p>
depicted is the third part of our project, which aims to secrete AANAT, an
+
enzyme that can produce a melatonin precursor.<o:p></o:p></span></i></p>
+
  
 
<p class=MsoNormal><span style='font-size:16.0pt;line-height:107%;font-family:
 
<p class=MsoNormal><span style='font-size:16.0pt;line-height:107%;font-family:
"Georgia",serif'><o:p>&nbsp;</o:p></span></p>
+
"Georgia",serif'>&nbsp;</span></p>
  
<p class=MsoNormal><b style='mso-bidi-font-weight:normal'><span
+
<p class=MsoNormal><b><span style='font-size:16.0pt;line-height:107%;
style='font-size:16.0pt;line-height:107%;font-family:"Georgia",serif'>Still not
+
font-family:"Georgia",serif'>Still not convinced? Then click </span></b><a
convinced? Then click </span></b><a
+
href="https://2015.igem.org/Team:UChicago/Description"><b><span
href="https://2015.igem.org/Team:UChicago/Description"><b style='mso-bidi-font-weight:
+
style='font-size:16.0pt;line-height:107%;font-family:"Georgia",serif'>here</span></b></a><b><span
normal'><span style='font-size:16.0pt;line-height:107%;font-family:"Georgia",serif'>here</span></b></a><b
+
style='font-size:16.0pt;line-height:107%;font-family:"Georgia",serif'> to learn
style='mso-bidi-font-weight:normal'><span style='font-size:16.0pt;line-height:
+
more!</span></b></p>
107%;font-family:"Georgia",serif'> to learn more!<o:p></o:p></span></b></p>
+
  
 
</div>
 
</div>
Line 991: Line 174:
  
 
</html>
 
</html>
 +
  
  
  
 
{{UChicago/Footer}}
 
{{UChicago/Footer}}

Latest revision as of 03:51, 9 October 2015

Team UChicago banner.jpg

Transplanting KaiABC: Reduce your jet lag with this one weird trick!

 

Do you see these images in your jet-lag-drunken nightmares? I know I do.

                                                                                                           

Have you ever felt tired as a weary traveler after a whole day of flying in an unfamiliar city? So doggone tired that you would do anything just to feel refreshed anything? Then just click here to buy the ultimate cure to jet lag! Or just give us your credit card information here and we’ll ship it to you pronto!

 

…Just kidding! We’re not trying to scam you here, we’re the UChicago 2015 iGEM team and we’re trying to create bacteria that can reduce or cure your jet lag (as well as sleeping disorders).

 

We actually have science to back us up! Currently, there exist no working 24-hour oscillator BioBricks. We are addressing this problem by transplanting the circadian rhythm-generating KaiABC system from cyanobacteria into E. coli and optimizing its function by controlling protein stoichiometry and adding accessory proteins. Such a BioBrick would be important for any application in which gene expression needs to be regulated on a 24-hour schedule. For example, it is often important to release a drug at a specific time of the day. As a proof-of-concept application, we implemented this clock as a potential solution to jet lag and sleep disorders by engineering a strain of E. coli that facilitates the production of a melatonin precursor in the gut on a 24-hour cycle. Thus, our project hopes to demonstrate the application of a robust KaiABC-based clock in timed drug dosage, as well as introduces an oscillator BioBrick into the synthetic biology community.

 

Figure 1: A) The first leg of our project focuses on reconstituting the KaiABC oscillator system. B) The second leg of our journey (see what we did there?) focuses on creating a readout system that can process KaiC phosphorylation state into actual promoter state. Not depicted is the third part of our project, which aims to secrete AANAT, an enzyme that can produce a melatonin precursor.

 

Still not convinced? Then click here to learn more!



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