Team:Freiburg/Protocols/Serum Preparation

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Serum preparation

adapted from: protocol proimmune

material: blood sample, centrifuge, cryovials, liquid nitrogen, filter tips
~ 90 min

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1. incubate blood sample in an upright position for 30 - 45 minutes at RT (maximum: 60 minutes)
2. centrifuge for 15 minutes at RT 1000 - 2000 rcf, do not use brake for stopping!
3. Inspect serum for turbidity. Turbid samples should be centrifuged and aspirated again to remove remaining insoluble matter.
4. aspirate supernatant (=serum) and aliquot into cryovials and flashfreeze in liquid nitrogen and store at –80 °C, take care not to disturb the cell layer or transfer any cells

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