Team:Freiburg/Protocols

• Home
• Project
• Team
• Results
• Human Practice
• Notebook

• Introduction
• Overview
• Diagnostics today
• The DiaCHIP
  • DNA Engineering
  • Cell-Free Expression
  • Protein Purification
  • Surface Chemistry
  • Optical Detection
• Outlook

• Members
• Acknowledgements
• Partners

• The DiaCHIP
  • Main Results
  • Diagnostics
  • Our Device
  • DNA Array
  • Cell-Free Expression
  • Binding on Surface
  • Modeling
• BioBricks
• Collaborations
• Interlab Study
• Achievements

• Science Fair
• Theater Project
• Public Opinion
• College Talk
• Ethics

• Labjournals
• Techniques
  • Gibson
  • E.coli Strains
• Protocols
• Materials
• Safety
• Wiki-Tricks
• Glossary

• Home
• Project
• Results
• Team
• Practice
• Notebook

Menu

text

next

Cloning

• Blunt End Ligation
• Competent Cells
• Cryo Stocks
• gBlock Procedure
• Agarose Gels
• Antibiotics
• Colony PCR
• Competent Cells
• Fusion PCR
• Gel Extraction
• Gibson Assembly
• Isopropanol Precipitation
• Ligation
• Miniprep
• PCR
• Restriction Digest
• Transformation

Cell-free Expression

• Preparation of an E.Coli lysate for Cell-free Expression
• Cell-free Expression general
• Cell-free Expression on PDMS Flow Cell
• Preparation of Terrific Broth
• Luciferase Assay
• Measurement of GFP Expression

Surface Chemistry

• Plasma Activation
• GOPTS Surface
• APTES-PDITC Surface
• Ni-NTA Surface
• HALO Surface (Silane)
• Glass Slide Preparation (full)
• iRIF Slide Preparation (full)
• Spotting
• Moulding Flowcells
• PDITC Surface on PDMS

Protein Purification & Analysis

• AmidoBlack
• Ni-NTA Bead Regeneration
• Buffers for Analysis
• Cell Lysis
• Desalting of Protein Solutions (Columns)
• DotBlot
• Freezing and Thawing Cells
• Microwave Destaining
• Overexpression
• Periplasmic Preparation
• Purification
• SDS-Page
• Blood Serum Preparation
• Transfection
• Western Blot

iRIf

• iRIf Measurement
• Protein Stamping
• iRIf Slide Cleaning

Interlab Study

• InterLab Protocol & Worksheet