All information on our parts can be found on this page
We've also included links to these side pages where we seperate our basic and composite parts
Full Parts List
|K1783000||pBAD-RBS-Tagged-Miraculin||Makes miraculin containing a transport tag for proper folding and a his-tag for purification||Composite|
|K1783001||Hok/Sok||Type I Toxin/antitoxin (TA) system, naturally used to maintain plasmids||Composite|
|K1783002||Constitutive unstable RFP||RFP under a constitutive promoter, with half life of 1 hour||Basic|
|K1783003||Hok-Sok+CU-RFP||Constitutive unstable RFP, under Hok-Sok regulation. Used as part of tests of Hok-Sok's ability to retain plasmids||Composite|
|K1783004||ε-cyclase||Converts lycopene into α-carotene||Basic|
|K1783005||ε-hydroxylase||Adds hydroxyl group to ε ring||Basic|
Combines BBa_K206000 (pBAD promoter) with BBa_K1033120 (B0034-ExpTag-Linker-Miraculin-Linker-6xHis). Codes for a His-tagged miraculin with export tag to periplasm. Induced with arabinose. Miraculin is a protein that interacts with our taste receptors, causing us to taste sour foods as sweet. This part was assembled using 3A Assembly as a training exercise for new UMaryland iGEM members.
Codes for the Hok-Sok Type I toxin-antitoxin system. This system naturally evolved in bacteria as a method to maintain plasmids. Hok-Sok is noted for its presence on the R1 plasmid, which contains genes that confer antibiotic resistance. Hok-Sok is believed to be used to maintain these plasmids in the absence of antibiotic.
Complete with a constitutive promoter, this fluorescent protein is expressed continuously in the cytoplasm. Due to the presence of a LVA degradation tag, this protein is subject to rapid targeting via proteases, with a half life of about 1 hour.
This part combines two of our previous parts: Hok-Sok TA cassette and Constitutive Unstable RFP. This testing device was used to measure the ability of the Hok-Sok maintenance system to force cells to retain plasmids. The presence of Hok-Sok adjacent to the constitutive promoter for unstable RFP was found to interfere with RFP production in a minor way.
Epsilon cyclase from the A. thaliana genome is capable of converting lycopene to gamma-carotene. A transport tag for the chloroplast at the N-terminus has been removed and the coding sequence has been codon-optimized for E. coli.
Epsilon cyclase from the A. thaliana genome is capable of converting alpha-carotene to alpha-cryptoxanthin. A transport tag for the chloroplast at the N-terminus has been removed and the coding sequence has been codon-optimized for E. coli.