Fast Protein Liquid Chromatography
Procedure:
- - Centrifuge cell lysate (balance with equal mass of water) for 60 minutes at 25000 rpm
- - Wash FPLC column with equilibration column (X2) with syringe
- - Wash out FLPC system with equilibration buffer
- - Wash affinity column with equilibration column with syringe
- - Wash affinity column with cobalt acetate with syringe
- - Wash off excess cobalt with syringe
- - Connect affinity column to FPLC system
- - Inject supernatant
- - Wash FPLC column with equilibration column (X2) with syringe