Difference between revisions of "Team:UMaryland/Composite Part"
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Revision as of 18:57, 28 August 2015
pBAD-B0034-ExpTag-Linker-Miraculin-Linker-6xHis. Adds a promoter to BBa_K1033120 in order to characterize it. Under arabinose induction, produces miraculin connected to an export tag and a His-tag via unstructured linkers. Miraculin is a taste modifying protein.
Produces unstable RFP under a constitutive promoter. This construct carries the hok-sok toxin-antitoxin (TA) plasmid maintenance system and was used to measure the ability of this alternative plasmid maintenance system to keep plasmids within the cell without the use of antibiotics.