Difference between revisions of "Team:Dundee/CGCraigon"
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<p class="journal-content"> Removal of the signaling peptide from the start of <i>OBP2A</i> and insertion of <i>OBP2A</i> into the biobrick vector pSB1C3. </p> | <p class="journal-content"> Removal of the signaling peptide from the start of <i>OBP2A</i> and insertion of <i>OBP2A</i> into the biobrick vector pSB1C3. </p> | ||
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<span class="box-title">22/6: PCR of pIDT-<I>OBP2A</I> using primers designed for the removal of the signaling peptide. </span> | <span class="box-title">22/6: PCR of pIDT-<I>OBP2A</I> using primers designed for the removal of the signaling peptide. </span> | ||
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<p><b>Aim of Experiment:</b> To transform pIDT-<i>OBP2A</i> into MC1061 <i> e.coli</i> strain </p> | <p><b>Aim of Experiment:</b> To transform pIDT-<i>OBP2A</i> into MC1061 <i> e.coli</i> strain </p> | ||
<p><b>Protocols Used:</b> <a data-toggle="modal" data-target="#transformation-modal" class="journal-protocol">Transformation </a> </p> | <p><b>Protocols Used:</b> <a data-toggle="modal" data-target="#transformation-modal" class="journal-protocol">Transformation </a> </p> | ||
Revision as of 17:06, 6 September 2015
Watch Our Introduction Video
Summary
Produced a plasmid preperation of pIDT-OBP2A in order to use it as template in PCR.
Aim of Experiment: To transform pIDT-OBP2A into MC1061 e.coli strain
Protocols Used: Transformation
Results: N/A
Next Steps: If the transformation is successful the next step will be to produce an overnight culture for plasmid preparation.
Aim of experiment: To produce an overnight culture of pIDT-OBP2A from positive colonies of MC1061 E.coli strain from the transformation done on the 17/6.
Protocols Used: Overnight Cultures
Results: N/A
Next Steps: Produce a plasmid preparation of pIDT-OBP2A from this overnight culture.
Aim of experiment: The overnight culture produced yesterday will now undergo a plasmid preparation in order to obtain the pIDT-OBP2A plasmid.
Protocols Used: Miniprep
Results: The plasmid preperation yeilded a concentration of 305.53 ng/ul.
Next Steps: To run a PCR using the purified pIDT-OBP2A plasmid as a template for amplification.
Summary
Removal of the signaling peptide from the start of OBP2A and insertion of OBP2A into the biobrick vector pSB1C3.
Aim of Experiment: To transform pIDT-OBP2A into MC1061 e.coli strain
Protocols Used: Transformation
Results: N/A
Next Steps: If the transformation is successful the next step will be to produce an overnight culture for plasmid preparation.
Aim of experiment: To produce an overnight culture of pIDT-OBP2A from positive colonies of MC1061 E.coli strain from the transformation done on the 17/6.
Protocols Used: Overnight Cultures
Results: N/A
Next Steps: Produce a plasmid preparation of pIDT-OBP2A from this overnight culture.
Aim of experiment: The overnight culture produced yesterday will now undergo a plasmid preparation in order to obtain the pIDT-OBP2A plasmid.
Protocols Used: Miniprep
Results: The plasmid preperation yeilded a concentration of 305.53 ng/ul.
Next Steps: To run a PCR using the purified pIDT-OBP2A plasmid as a template for amplification.
