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| | + | {{GlasgowNavbar}} |
| | <html> | | <html> |
| − | <body> | + | <meta name="viewport" content="width=device-width, initial-scale=1"> |
| | + | <head> |
| | + | <meta charset="utf-8"> |
| | + | <meta http-equiv="X-UA-Compatible" content="IE=edge"> |
| | + | <meta name="viewport" content="width=device-width, initial-scale=1"> |
| | + | <!-- The above 3 meta tags *must* come first in the head; any other head content must come *after* these tags --> |
| | + | <meta name="description" content=""> |
| | + | <meta name="author" content=""> |
| | + | <link href="https://2015.igem.org/Team:Glasgow/bootstrap.min.css?action=raw&ctype=text/css" rel="stylesheet"> |
| | + | <script src="https://2015.igem.org/Team:Glasgow/bootstrap.min.js?action=raw&ctype=text/css"></script> |
| | + | <script src="https://ajax.googleapis.com/ajax/libs/jquery/1.11.3/jquery.min.js"></script> |
| | + | <!-- Custom styles for this template --> |
| | + | <link href="carousel.css" rel="stylesheet"> |
| | + | <link href='http://fonts.googleapis.com/css?family=Unkempt' rel='stylesheet' type='text/css'> |
| | + | </head> |
| | <style> | | <style> |
| − | body {
| |
| − | font-family: "Roboto", sans-serif;
| |
| − | font-weight: 400;
| |
| − | }
| |
| | .firstHeading{ | | .firstHeading{ |
| | display:none; | | display:none; |
| | } | | } |
| − | /*===== Vertical Timeline =====*/
| + | #bodybackground { |
| − | #content{
| + | background:url(https://static.igem.org/mediawiki/2015/7/7c/GLasgow_7323.jpg) no-repeat center center; |
| − | height:90px;
| + | font-family: 'Nixie One', Helvetica, Arial, sans-serif; |
| − | padding: 0px 0px 0px 0px; | + | font-size: 50px; |
| − | border-left: 0px solid #444444; | + | height:45vh; |
| − | border-right:0px solid #444444; | + | background-size:cover; |
| − | background:transparent;
| + | |
| − | width:auto;
| + | |
| − | height: auto; | + | |
| − | }
| + | |
| − | #conference-timeline {
| + | |
| − | position: relative; | + | |
| − | max-width: 920px;
| + | |
| − | width: 100%;
| + | |
| − | margin: 0 auto;
| + | |
| | } | | } |
| − | | + | @font-face { |
| − | #conference-timeline .timeline-start,
| + | font-family: 'Nixie One'; |
| − | #conference-timeline .timeline-end {
| + | font-style: normal; |
| − | display: table;
| + | font-weight: 400; |
| − | font-family: "Roboto", sans-serif; | + | src: local('Nixie One'), local('NixieOne-Regular'), url(http://fonts.gstatic.com/s/nixieone/v7/gQ0PT3rPU_Et2ExNz-ahmpBw1xU1rKptJj_0jans920.woff2) format('woff2'); |
| − | font-size: 20px; | + | unicode-range: U+0000-00FF, U+0131, U+0152-0153, U+02C6, U+02DA, U+02DC, U+2000-206F, U+2074, U+20AC, U+2212, U+2215, U+E0FF, U+EFFD, U+F000; |
| − | font-weight: 600; | + | |
| − | background: #00b0bd; | + | |
| − | padding: 20px 23px;
| + | |
| − | color: #fff; | + | |
| − | max-width: 5%;
| + | |
| − | width: 100%;
| + | |
| − | text-align: center;
| + | |
| − | margin: 0 auto;
| + | |
| | } | | } |
| − | | + | @font-face { |
| − | #conference-timeline .conference-center-line {
| + | font-family: 'League Script'; |
| − | position: absolute; | + | font-style: normal; |
| − | width: 3px;
| + | |
| − | height: 100%;
| + | |
| − | top: 0;
| + | |
| − | left: 50%;
| + | |
| − | margin-left: -2px;
| + | |
| − | background: #00b0bd; | + | |
| − | z-index: -1;
| + | |
| − | }
| + | |
| − | | + | |
| − | #conference-timeline .conference-timeline-content {
| + | |
| − | padding-top: 67px;
| + | |
| − | padding-bottom: 67px;
| + | |
| − | }
| + | |
| − | | + | |
| − | .timeline-article {
| + | |
| − | width: 100%;
| + | |
| − | height: 100%;
| + | |
| − | position: relative;
| + | |
| − | overflow: hidden;
| + | |
| − | margin: 20px 0;
| + | |
| − | }
| + | |
| − | | + | |
| − | .timeline-article .content-left-container,
| + | |
| − | .timeline-article .content-right-container {
| + | |
| − | max-width: 44%;
| + | |
| − | width: 100%;
| + | |
| − | }
| + | |
| − | | + | |
| − | .timeline-article .timeline-author {
| + | |
| − | display: block;
| + | |
| | font-weight: 400; | | font-weight: 400; |
| − | font-size: 14px; | + | src: local('League Script'), local('LeagueScript'), url(http://fonts.gstatic.com/s/leaguescript/v7/wnRFLvfabWK_DauqppD6vTLKmjGCnkmx1ziy0T7wKLI.woff2) format('woff2'); |
| − | line-height: 24px; | + | unicode-range: U+0000-00FF, U+0131, U+0152-0153, U+02C6, U+02DA, U+02DC, U+2000-206F, U+2074, U+20AC, U+2212, U+2215, U+E0FF, U+EFFD, U+F000; |
| − | color: #242424;
| + | |
| − | text-align: right;
| + | |
| | } | | } |
| − | | + | .container3 { |
| − | .timeline-article .content-left, | + | display: flex; |
| − | .timeline-article .content-right {
| + | flex-direction: row; |
| − | position: relative;
| + | flex-wrap: wrap; |
| − | width: auto;
| + | justify-content: center; |
| − | border: 1px solid #ddd;
| + | align-items: center; |
| − | background-color: #fff;
| + | line-height:7vh; |
| − | box-shadow: 0 1px 3px rgba(0, 0, 0, .03);
| + | padding-top:50px; |
| − | padding: 27px 25px;
| + | |
| | } | | } |
| − | | + | .sign { |
| − | .timeline-article p { | + | -ms-transform: rotate(-3deg); |
| − | margin: 0 0 0 60px; | + | -webkit-transform: rotate(-3deg); |
| − | padding: 0; | + | transform: rotate(-3deg); |
| − | font-weight: 400;
| + | margin-top:10vh; |
| − | color: #242424; | + | |
| − | font-size: 14px;
| + | |
| − | line-height: 24px; | + | |
| − | position: relative;
| + | |
| | } | | } |
| − | | + | #title { |
| − | .timeline-article p span.article-number {
| + | font-size: 10vh; |
| − | position: absolute;
| + | |
| − | font-weight: 300;
| + | |
| − | font-size: 44px; | + | |
| − | top: 10px;
| + | |
| − | left: -60px;
| + | |
| − | color: #00b0bd;
| + | |
| | } | | } |
| − | | + | #trav { |
| − | .timeline-article .content-left-container { | + | -webkit-animation: blink 0.01s infinite alternate; |
| − | float: left; | + | -moz-animation: blink 0.01s infinite alternate; |
| | + | -o-animation: blink 0.01s infinite alternate; |
| | + | animation: blink 0.01s infinite alternate; |
| | } | | } |
| − | | + | #fade { |
| − | .timeline-article .content-right-container { | + | opacity: 0.6; |
| − | float: right; | + | color: #ebffff; |
| | + | text-shadow: 2px 2px 1px rgba(0,0,0,0.3), 0 0px 15px #fff, 0 0 10px #38eeff, 0 0 50px #38eeff; |
| | + | -webkit-animation: fade 3s infinite alternate; |
| | + | -moz-animation: fade 3s infinite alternate; |
| | + | -o-animation: fade 3s infinite alternate; |
| | + | animation: fade 3s infinite alternate; |
| | } | | } |
| − | | + | .neon-blue { |
| − | .timeline-article .content-left:before, | + | margin: 0 auto; |
| − | .timeline-article .content-right:before {
| + | text-align: center; |
| − | position: absolute; | + | color: #ebffff; |
| − | top: 20px; | + | text-shadow: 2px 2px 1px rgba(0,0,0,0.3), 0 0px 15px #fff, 0 0 10px #38eeff, 0 0 50px #38eeff; |
| − | font-size: 23px; | + | font-size:4vw; |
| − | font-family: "FontAwesome"; | + | |
| − | color: #fff; | + | |
| | } | | } |
| − | | + | .neon-purple { |
| − | .timeline-article .content-left:before { | + | font-family: 'League Script', Helvetica, Arial, sans-serif; |
| − | content: "\f0da"; | + | font-size: 130px; |
| − | right: -8px; | + | margin: 0 auto; |
| | + | text-align: center; |
| | + | color: #6CA7FF; |
| | + | text-shadow: 2px 2px 1px rgba(0,0,0,0.5), 0 0 5px #fff, 0 0 10px #7d26cd, 0 0 35px #7d26cd; |
| | + | font-size:8vh; |
| | } | | } |
| − | | + | @-moz-keyframes blink { |
| − | .timeline-article .content-right:before {
| + | 70% { |
| − | content: "\f0d9"; | + | opacity: 0.7; |
| − | left: -8px; | + | } |
| | } | | } |
| − | | + | @-webkit-keyframes blink { |
| − | .timeline-article .meta-date {
| + | 45% { |
| − | position: absolute; | + | opacity: 0.5; |
| − | top: 0;
| + | } |
| − | left: 50%; | + | |
| − | width: 70px;
| + | |
| − | height: 70px;
| + | |
| − | margin-left: -31px;
| + | |
| − | color: #fff;
| + | |
| − | border-radius: 100%;
| + | |
| − | background: #00b0bd;
| + | |
| | } | | } |
| − | | + | @-o-keyframes blink { |
| − | .timeline-article .meta-date .date,
| + | 70% { |
| − | .timeline-article .meta-date .month {
| + | opacity: 0.7; |
| − | display: block; | + | } |
| − | text-align: center;
| + | |
| − | font-weight: 900; | + | |
| | } | | } |
| − | | + | @keyframes blink { |
| − | .timeline-article .meta-date .date {
| + | 70% { |
| − | font-size: 30px;
| + | opacity: 0.7; |
| − | line-height: 40px; | + | } |
| | } | | } |
| − | | + | @-moz-keyframes fade { |
| − | .timeline-article .meta-date .month {
| + | 40% { |
| − | font-size: 18px; | + | opacity: 0.8; |
| − | line-height: 10px; | + | } |
| | + | 42% { |
| | + | opacity: 0.1; |
| | + | } |
| | + | 43% { |
| | + | opacity: 0.8; |
| | + | } |
| | + | 45% { |
| | + | opacity: 0.1; |
| | + | } |
| | + | 46% { |
| | + | opacity: 0.8; |
| | + | } |
| | } | | } |
| − | | + | @-webkit-keyframes fade { |
| − | /*===== // Vertical Timeline =====*/
| + | 40% { |
| − | | + | opacity: 0.8; |
| − | /*===== Navigation Bar =====*/
| + | |
| − | | + | |
| − | nav {
| + | |
| − | background-color:black;
| + | |
| − | height:50px;
| + | |
| − | padding: 0px;
| + | |
| − | margin: 0px;
| + | |
| − | position: fixed;
| + | |
| − | width:100%;
| + | |
| − | top:15px;
| + | |
| − | left:0;
| + | |
| − | z-index:99;
| + | |
| − | }
| + | |
| − |
| + | |
| − | nav ul {
| + | |
| − | height:50px;
| + | |
| − | width: 1120px;
| + | |
| − | margin: 0 auto;
| + | |
| − | padding: 0px;
| + | |
| − | }
| + | |
| − |
| + | |
| − | li a img {
| + | |
| − | height:50px;
| + | |
| − | width: 80px;
| + | |
| − | padding: 0px;
| + | |
| − | margin: 0px;
| + | |
| − | }
| + | |
| − |
| + | |
| − | nav ul li {
| + | |
| − | list-style: none;
| + | |
| − | width:160px;
| + | |
| − | max-height: 50px;
| + | |
| − | float: left;
| + | |
| − | text-align: center;
| + | |
| − | padding: 0px;
| + | |
| − | margin: 0px;
| + | |
| − | }
| + | |
| − |
| + | |
| − | nav ul li a {
| + | |
| − | text-decoration: none;
| + | |
| − | color: white;
| + | |
| − | line-height: 50px;
| + | |
| − | display:block;
| + | |
| − | }
| + | |
| − |
| + | |
| − | nav ul li a:visited {
| + | |
| − | color:white;
| + | |
| − | }
| + | |
| − |
| + | |
| − | li a:hover {
| + | |
| − | background-color:white;
| + | |
| − | color:black;
| + | |
| − | height:50px;
| + | |
| − | }
| + | |
| − |
| + | |
| − | /*===== //Navigation Bar ====*/
| + | |
| − | /*===== Resonsive Vertical Timeline =====*/
| + | |
| − | | + | |
| − | @media only screen and (max-width: 830px) {
| + | |
| − | #conference-timeline .timeline-start, #conference-timeline .timeline-end { | + | |
| − | margin: 0; | + | |
| | } | | } |
| − | #conference-timeline .conference-center-line { | + | 42% { |
| − | margin-left: 0; | + | opacity: 0.1; |
| − | left: 50px;
| + | |
| | } | | } |
| − | .timeline-article .meta-date { | + | 43% { |
| − | margin-left: 0; | + | opacity: 0.8; |
| − | left: 20px;
| + | |
| | } | | } |
| − | .timeline-article .content-left-container, | + | 45% { |
| − | .timeline-article .content-right-container {
| + | opacity: 0.1; |
| − | max-width: 100%; | + | |
| − | width: auto;
| + | |
| − | float: none;
| + | |
| − | margin-left: 110px;
| + | |
| − | min-height: 53px;
| + | |
| | } | | } |
| − | .timeline-article .content-left-container { | + | 46% { |
| − | margin-bottom: 20px; | + | opacity: 0.8; |
| | } | | } |
| − | .timeline-article .content-left,
| + | } |
| − | .timeline-article .content-right { | + | @-o-keyframes fade { |
| − | padding: 10px 25px; | + | 40% { |
| − | min-height: 65px;
| + | opacity: 0.8; |
| | } | | } |
| − | .timeline-article .content-left:before { | + | 42% { |
| − | content: "\f0d9"; | + | opacity: 0.1; |
| − | right: auto;
| + | |
| − | left: -8px;
| + | |
| | } | | } |
| − | .timeline-article .content-right:before { | + | 43% { |
| − | display: none; | + | opacity: 0.8; |
| | + | } |
| | + | 45% { |
| | + | opacity: 0.1; |
| | + | } |
| | + | 46% { |
| | + | opacity: 0.8; |
| | } | | } |
| | } | | } |
| − | | + | @keyframes fade { |
| − | @media only screen and (max-width: 400px) { | + | 40% { |
| − | .timeline-article p { | + | opacity: 0.8; |
| − | margin: 0; | + | |
| | } | | } |
| − | .timeline-article p span.article-number { | + | 42% { |
| − | display: none; | + | opacity: 0.1; |
| | + | } |
| | + | 43% { |
| | + | opacity: 0.8; |
| | + | } |
| | + | 45% { |
| | + | opacity: 0.1; |
| | + | } |
| | + | 46% { |
| | + | opacity: 0.8; |
| | } | | } |
| | } | | } |
| − | /*===== // Resonsive Vertical Timeline =====*/
| |
| − | </style>
| |
| | | | |
| − | <!-------HTML----------->
| + | /*Table style*/ |
| − | <meta charset="UTF-8" />
| + | td { |
| − | <title>Responsive Vertical Timeline</title>
| + | padding:10px; |
| − |
| + | text-align: center; |
| − | <!-- Fonts -->
| + | border: 1px solid black; |
| − | <link href='http://fonts.googleapis.com/css?family=Roboto:400,300,400italic,700,900' rel='stylesheet' type='text/css'>
| + | } |
| − | <link href="http://cdnjs.cloudflare.com/ajax/libs/font-awesome/4.2.0/css/font-awesome.min.css" rel="stylesheet" type="text/css">
| + | |
| − | <!-- // Fonts -->
| + | |
| − | </head>
| + | |
| | | | |
| − | <body>
| + | table { |
| − | <!-- Navigation bar -->
| + | padding:50px; |
| − | <nav>
| + | margin:20px 91px; |
| − | <ul>
| + | } |
| − | <li><div></div><a class="project" href='#'>Project</a></li>
| + | |
| − | <li><div></div><a class="team" href='#'>Team</a></li>
| + | |
| − | <li><div></div><a class="journal" href='#'>Journal</a></li>
| + | |
| − | <li><a href='#'><img class="main" src="uni.jpg"/></a></li>
| + | |
| − | <li><div></div><a class="modelling" href='#'>Modelling</a></li>
| + | |
| − | <li><div></div><a class="application" href='#'>Application</a></li>
| + | |
| − | <li><div></div><a class="contact" href='#'>Contact</a></li>
| + | |
| − | </ul>
| + | |
| − | </nav>
| + | |
| − | <!-- //Navigation bar -->
| + | |
| | | | |
| − | <!-- Vertical Timeline -->
| + | th { |
| − | <section id="conference-timeline">
| + | text-align: center; |
| − | <div class="conference-center-line"></div>
| + | } |
| − | <div class="conference-timeline-content">
| + | |
| − | <!--------JUNE------->
| + | |
| − | <br/>
| + | |
| − | <br/>
| + | |
| − | <br/>
| + | |
| − | <br/>
| + | |
| − | <br/>
| + | |
| − | <br/>
| + | |
| − | <br/>
| + | |
| − | <br/>
| + | |
| − | <br/>
| + | |
| − | <br/>
| + | |
| − | <br/>
| + | |
| − | <br/>
| + | |
| − | <div class="timeline-start" id="Week1">Week1</div>
| + | |
| − | <div class="timeline-article">
| + | |
| − | <div class="content-left-container">
| + | |
| − | <div class="content-left">
| + | |
| − | <p>We went to Dundee University to attend the Scotland IGEM team's meeting. We had so much fun in the meeting, sharing different ideas with each team.</p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="content-right-container">
| + | |
| − | <div class="content-right">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2015/e/eb/2015-Glasgow-timeline1.jpg" style="max-width:350px;">
| + | |
| − | <p></p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="meta-date">
| + | |
| − | <span class="date">9th</span>
| + | |
| − | <span class="month">Jun</span>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="timeline-article">
| + | |
| − | <div class="content-left-container">
| + | |
| − | <div class="content-left">
| + | |
| − | <p>The 4 strains of E. coli used are: MGI655ZI (which has tetR, and lacIq, DS941 (recF-), TOP10 (recA-), DH5α (recA-). We designed Phlf and SrpR promoters as BioBricks to be ordered from IDT as if their prefix and suffix were cut with EcoRI and SpeI.</p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="content-right-container">
| + | |
| − | <div class="content-right">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2015/4/42/2015-Glasgow-timeline2.jpg" style="max-width:350px;">
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="meta-date">
| + | |
| − | <span class="date">15th</span>
| + | |
| − | <span class="month">Jun</span>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="timeline-article">
| + | |
| − | <div class="content-left-container">
| + | |
| − | <div class="content-left">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2015/5/5f/2015-Glasgow-beijing1.png" style="max-width:350px;">
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="content-right-container">
| + | |
| − | <div class="content-right">
| + | |
| − | <p> We grew bacteria from a single colony from an agar plate in liquid culture to prepare for the dilution series. We designed the Phlf and SrpR respressor sequences with a prefix, B0032 ribosome binding site, TACTAG scar sequence, the repressor itself, TACTAGAG scar sequence, B0010 extended terminator with the help of Steve, and suffix.</p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="meta-date">
| + | |
| − | <span class="date">16th</span>
| + | |
| − | <span class="month">Jun</span>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="timeline-article">
| + | |
| − | <div class="content-left-container">
| + | |
| − | <div class="content-left">
| + | |
| − | <p>We made TOP10 (which lacks methylation and restriction system, which makes it difficult to ‘cross’ with strains that have the systems) and DH5α (which lacks a restriction system, but has a modification system, ergo, DNA from it can be transferred to other cells) competent for transformation. Since we needed to have the cells in exponential growth phase, we pipetted 4 mL of overnight culture into 20 mL of fresh broth. We made chloramphenicol agar plates. We are also tested our dilution series on non-selective agar plates.</p>
| + | |
| − | </div>
| + | |
| − | </div>
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| − | <div class="content-right-container">
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| − | <div class="content-right">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2015/b/b6/2015-Glasgow-timeline4.jpg" style="max-width:350px;">
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="meta-date">
| + | |
| − | <span class="date">17th</span>
| + | |
| − | <span class="month">Jun</span>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="timeline-article">
| + | |
| − | <div class="content-left-container">
| + | |
| − | <div class="content-left">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2015/1/16/2015-Glasgow-timeline5.jpg" style="max-width:350px;">
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="content-right-container">
| + | |
| − | <div class="content-right">
| + | |
| − | <p>For TOP10 we had a transformation efficiency of 1.26 x 106 colonies/µg of DNA. For DH5α the control was not transformed. DH5α can be 40 times less competent than TOP10, which would explain our result.
| + | |
| − | TOP10 is mutant in the repressor for lacI, while DH5α has one copy, so they cannot fully repress the (multiply copied) plasmid. Therefore with IPTG there is no difference in GFP expression in TOP10, and there may be some difference in DH5α.
| + | |
| − | </p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="meta-date">
| + | |
| − | <span class="date">18th</span>
| + | |
| − | <span class="month">Jun</span>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="timeline-article">
| + | |
| − | <div class="content-left-container">
| + | |
| − | <div class="content-left">
| + | |
| − | <p>We did MiniPrep of the BioBrick transformants and GFP without a promoter. We kept a gel over the weekend in the refrigerator.</p>
| + | |
| − | </div>
| + | |
| − | </div>
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| − | <div class="content-right-container">
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| − | <div class="content-right">
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| − | </div>
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| − | </div>
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| − | <div class="meta-date">
| + | |
| − | <span class="date">19th</span>
| + | |
| − | <span class="month">Jun</span>
| + | |
| − | </div>
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| − | </div>
| + | |
| − | <div class="timeline-start" id="Week2">Week2</div>
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| − | <div class="timeline-article">
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| − | <div class="content-left-container">
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| − | <div class="content-left">
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| − | </div>
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| − | </div>
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| − | <div class="content-right-container">
| + | |
| − | <div class="content-right">
| + | |
| − | <p>LuxD encodes a transferase, luxE is a synthetase, and luxC is the reductase.
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| − | If the association map on photobiology of the DEG complex is correct, the whole tetrameric complex may be temperature-sensitive.
| + | |
| − | </p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="meta-date">
| + | |
| − | <span class="date">22th</span>
| + | |
| − | <span class="month">Jun</span>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="timeline-article">
| + | |
| − | <div class="content-left-container">
| + | |
| − | <div class="content-left">
| + | |
| − | <p>We transformed bacteria that glowed (F1 and F3 are glowing, while F2 and F4 are controls). We are going to measure their glow tomorrow with a more appropriate exposure configuration.We made competent cells.We were going over primer design.
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| − | </p>
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| − | </div>
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| − | </div>
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| − | <div class="content-right-container">
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| − | <div class="content-right">
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="meta-date">
| + | |
| − | <span class="date">23th</span>
| + | |
| − | <span class="month">Jun</span>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="timeline-article">
| + | |
| − | <div class="content-left-container">
| + | |
| − | <div class="content-left">
| + | |
| − | </div>
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| − | </div>
| + | |
| − | <div class="content-right-container">
| + | |
| − | <div class="content-right">
| + | |
| − | <p>We transformed cultures with BioBricks (for us and for the master’s lab) and ligations. To make bacteria glow, we have repeated the same experiment as yesterday. After 1h in the 25°C incubator we did not observe bacteria to glow. After 5h in the incubator bacteria were glowing bright.
| + | |
| − | We ordered primers for uirR, uirS and all of the lux genes: LuxA, LuxB, LuxC, LuxD, LuxE, LuxG. For the forward primers we included B0032 ribosome binding site and scar. In addition, we ordered a g-block for plsiR. Sean gave us recipes for PCR and Restriction.
| + | |
| − | </p>
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| − | </div>
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| − | </div>
| + | |
| − | <div class="meta-date">
| + | |
| − | <span class="date">24th</span>
| + | |
| − | <span class="month">Jun</span>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="timeline-article">
| + | |
| − | <div class="content-left-container">
| + | |
| − | <div class="content-left">
| + | |
| − | <p>We made overnight cultures.</p>
| + | |
| − | </div>
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| − | </div>
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| − | <div class="content-right-container">
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| − | <div class="content-right">
| + | |
| − | </div>
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| − | </div>
| + | |
| − | <div class="meta-date">
| + | |
| − | <span class="date">25th</span>
| + | |
| − | <span class="month">Jun</span>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="timeline-article">
| + | |
| − | <div class="content-left-container">
| + | |
| − | <div class="content-left">
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="content-right-container">
| + | |
| − | <div class="content-right">
| + | |
| − | <p>We made glycerol stocks from the BioBricks as well as MiniPreps </p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="meta-date">
| + | |
| − | <span class="date">26th</span>
| + | |
| − | <span class="month">Jun</span>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="timeline-start" id="Week3">Week3</div>
| + | |
| − | <div class="timeline-article">
| + | |
| − | <div class="content-left-container">
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| − | <div class="content-left">
| + | |
| − | <p>We made restriction digests of the minipreps and annealed R0011. B0032 oligos. After we ran the gel electrophoresis and extracted fragments from the gel. We left ligations overnight and made overnight cultures for making electrocompetent cells.</p>
| + | |
| − | </div>
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| − | </div>
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| − | <div class="content-right-container">
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| − | <div class="content-right">
| + | |
| − | </div>
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| − | </div>
| + | |
| − | <div class="meta-date">
| + | |
| − | <span class="date">29th</span>
| + | |
| − | <span class="month">Jun</span>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="timeline-article">
| + | |
| − | <div class="content-left-container">
| + | |
| − | <div class="content-left">
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="content-right-container">
| + | |
| − | <div class="content-right">
| + | |
| − | <p>PCR was done to amplify UirR and lux genes (three separate reactions were done) and attempted to make electrocompetent cells. We ligated G-blocks of PhlF and SrpR into pSB1C3.</p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="meta-date">
| + | |
| − | <span class="date">30th</span>
| + | |
| − | <span class="month">Jun</span>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="timeline-article">
| + | |
| − | <div class="content-left-container">
| + | |
| − | <div class="content-left">
| + | |
| − | <p>We made 2 other PCR reactions and tested all PCR products on gel. We found out that none of the amplifications worked except for the one of luxB. We attempted to transform electrocompetent with ligations and we made overnights for making of chemically competent cells in case our transformations fail.</p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="content-right-container">
| + | |
| − | <div class="content-right">
| + | |
| − | <p></p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="meta-date">
| + | |
| − | <span class="date">1st</span>
| + | |
| − | <span class="month">Jul</span>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="timeline-article">
| + | |
| − | <div class="content-left-container">
| + | |
| − | <div class="content-left">
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="content-right-container">
| + | |
| − | <div class="content-right">
| + | |
| − | <p>We redid UirS PCR and checked it on gel. This time our reaction successfully yielded a product of the expected size. (What did we change, was it primer concentrations?). We attempted to transform cells again, only this time with chemically competent cells. The large centrifuge was broken, so we divided the work and used a smaller centrifuge in the cold room. We redid digests of PhlF and SrpR G-block, checked them on gel, and redid ligations into pSB1C3.</p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="meta-date">
| + | |
| − | <span class="date">2nd</span>
| + | |
| − | <span class="month">Jul</span>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="timeline-article">
| + | |
| − | <div class="content-left-container">
| + | |
| − | <div class="content-left">
| + | |
| − | <p>The transformation of chemically competent cells also failed (no growth of positive controls, no growth of anything we hoped to grow) and we do not know why. The plan for today is to insert UirS in pCR2.1TOPO. We are also planning to check gel purified PhlF and SrpR on gel. PLSIR G-block is to be resuspended and digested.</p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="content-right-container">
| + | |
| − | <div class="content-right">
| + | |
| − | <p></p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="meta-date">
| + | |
| − | <span class="date">3th</span>
| + | |
| − | <span class="month">Jul</span>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="timeline-start" id="Week4">Week4</div>
| + | |
| − | <div class="timeline-article">
| + | |
| − | <div class="content-left-container">
| + | |
| − | <div class="content-left">
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="content-right-container">
| + | |
| − | <div class="content-right">
| + | |
| − | <p>We ran the PCR products of UirR and luxA-G on a gel to check if our PCR worked. We also ligated PLSIR into pSB1C3. We made competent cells and set up overnight cultures and made buffer for the ligation of UirS into TOPO TA.</p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="meta-date">
| + | |
| − | <span class="date">6th</span>
| + | |
| − | <span class="month">Jul</span>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="timeline-article">
| + | |
| − | <div class="content-left-container">
| + | |
| − | <div class="content-left">
| + | |
| − | <p>We made competent cells and transformed ligations (which ligations?). We ligated the purified UirS into TOPO TA and redid the luxE PCR. We ran digested UirR, luxA,B,C,D,G on a gel and extracted them. The digests were then ligated into pSB1C3. We set up overnights of registry order</p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="content-right-container">
| + | |
| − | <div class="content-right">
| + | |
| − | <p></p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="meta-date">
| + | |
| − | <span class="date">7th</span>
| + | |
| − | <span class="month">Jul</span>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="timeline-article">
| + | |
| − | <div class="content-left-container">
| + | |
| − | <div class="content-left">
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="content-right-container">
| + | |
| − | <div class="content-right">
| + | |
| − | <p>We ran a gel of the PCR product extract and set up ligations (which ligations?). We made minipreps of the overnight cultures and made competent cells and transformed ligations. The transformants were set up as overnights.</p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="meta-date">
| + | |
| − | <span class="date">8th</span>
| + | |
| − | <span class="month">Jul</span>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="timeline-article">
| + | |
| − | <div class="content-left-container">
| + | |
| − | <div class="content-left">
| + | |
| − | <p>We made minipreps from the overnight cultures: TOPO TA, control, lig. 17, lig. 18, lig. 20) and we ordered primers for site-directed mutagenesis. We digested (our minipreps?), ran them on a gel, extracted them, and set up ligations. We transformed cells with the luxE ligation and set up overnights of transformants.</p>
| + | |
| − | </div>
| + | |
| − | </div>
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| − | <div class="content-right-container">
| + | |
| − | <div class="content-right">
| + | |
| − | <p></p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="meta-date">
| + | |
| − | <span class="date">9th</span>
| + | |
| − | <span class="month">Jul</span>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="timeline-article">
| + | |
| − | <div class="content-left-container">
| + | |
| − | <div class="content-left">
| + | |
| − | </div>
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| − | </div>
| + | |
| − | <div class="content-right-container">
| + | |
| − | <div class="content-right">
| + | |
| − | <p>We did minipreps and digested ligations 21, 22, 24, and ran them on a gel. We checked yesterday’s extractions on a gel.</p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="meta-date">
| + | |
| − | <span class="date">10th</span>
| + | |
| − | <span class="month">Jul</span>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="timeline-start" id="Week5">Week5</div>
| + | |
| − | <div class="timeline-article">
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| − | <div class="content-left-container">
| + | |
| − | <div class="content-left">
| + | |
| − | <p>We digested and ran a gel to check for inversion of the UirS PCR product in the TOPO TA plasmid. We made TOP10 overnights to make competent cells for UirS/TOPO TA, PLSIR.I13500/pSB1C3, PLSIR.E5501/pSB1C3, R0011N.Phlf/pSB1C3, R0011N. SrpR/pSB1C3, and controls (lig. 36, 38, 41, and 43. We also made multiple overnights of UirR/pSB1C3, LuxA/pSB1C3, LuxB/pSB1C3, LuxC/pSB1C3, LuxD/pSB1C3, LuxE/pSB1C3, LuxG/pSB1C3. We ran luxA-G PCR products on a gel and purified them.</p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="content-right-container">
| + | |
| − | <div class="content-right">
| + | |
| − | <p></p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="meta-date">
| + | |
| − | <span class="date">13th</span>
| + | |
| − | <span class="month">Jul</span>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | <div class="timeline-article">
| + | |
| − | <div class="content-left-container">
| + | |
| − | <div class="content-left">
| + | |
| − | </div>
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| − | </div>
| + | |
| − | <div class="content-right-container">
| + | |
| − | <div class="content-right">
| + | |
| − | <p>We checked ligations with digests and transformed ligations of R0011N with Phlf and SrpR in pSB1C3 into TOP10 (these are ligations 35, 36, 37, and 38)</p>
| + | |
| − | </div>
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| − | </div>
| + | |
| − | <div class="meta-date">
| + | |
| − | <span class="date">14th</span>
| + | |
| − | <span class="month">Jun</span>
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| − | </div>
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| − | <div class="timeline-article">
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| − | <p></p>
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| − | <div class="meta-date">
| + | |
| − | <span class="date">30th</span>
| + | |
| − | <span class="month">Jun</span>
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| − | <p></p>
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| − | <div class="meta-date">
| + | |
| − | <span class="date">30th</span>
| + | |
| − | <span class="month">Jun</span>
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| − | <p></p>
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| + | |
| − | <span class="date">30th</span>
| + | |
| − | <span class="month">Jun</span>
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| − | <p></p>
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| + | |
| − | <span class="date">30th</span>
| + | |
| − | <span class="month">Jun</span>
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| − | <div class="content-right">
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| − | <span class="month">Jun</span>
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| | | | |
| − |
| |
| − | </div>
| |
| − | </section>
| |
| − |
| |
| − |
| |
| − |
| |
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| |
| − | <head>
| |
| − | <meta charset="utf-8">
| |
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| |
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| |
| − | font-size: 20px;
| |
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| |
| − | #Week1 #Week2 #Week3 #Week4#Week5#Week6#Week7#Week8#Week9#Week10{
| |
| − | margin-left: 5px;
| |
| − | }
| |
| − | }
| |
| | </style> | | </style> |
| − | </head> | + | </head> |
| − | <body data-spy="scroll" data-target="#myScrollspy" data-offset-top="100">
| + | <body> |
| − | <div class=" row" style="margin-top: 30px;"> | + | </br> |
| − | | + | <div id="bodybackground"> |
| − | | + | |
| − | <ul class="nav nav-pills nav-stacked"> | + | |
| − | <li class="active"><a href="#Week1">Week1</a></li>
| + | <div class="neon-blue" id="title">Glas<span id="fade">glow</span> </div> |
| − | <li><a href="#Week2">Week2</a></li>
| + | <div class="neon-blue"><br><span class="neon-purple"><strong>Parts</strong></span></div> |
| − | <li><a href="#Week3">Week3</a></li>
| + | </div> |
| − | <li><a href="#Week4">Week4</a></li>
| + | </div> |
| − | <li><a href="#Week5">Week5</a></li>
| + | |
| − | <li><a href="#Week6">Week6</a></li>
| + | |
| − | <li><a href="#Week7">Week7</a></li>
| + | |
| − | <li><a href="#Week8">Week8</a></li>
| + | |
| − | <li><a href="#Week9">Week9</a></li>
| + | |
| − | <li><a href="#Week10">Week10</a></li>
| + | |
| − | </ul>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| | </div> | | </div> |
| − | </div>
| |
| − | <!----------Random Page------>
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| |
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| |
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| |
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| |
| − | background-size:cover;
| |
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| |
| − | -moz-background-size:cover;
| |
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| − | <script>
| |
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| |
| − | var classCycle=['imageCycle1','imageCycle2'];
| |
| | | | |
| − | var randomNumber = Math.floor(Math.random() * classCycle.length);
| + | <div id="content" class="mw-body" role="main"> |
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| + | <a id="top"></a> |
| − |
| + | <h1 id="firstHeading" class="firstHeading"> |
| − | $('body').addClass(classToAdd);
| + | <span dir="auto">Team:Glasgow/Parts test</span> |
| | + | </h1> |
| | + | <div id="bodyContent"> |
| | + | <div id="mw-content-text" lang="en" dir="ltr" class="mw-content-ltr"><h2><span class="mw-headline" id="Summary_table" style="text-align:center;">Summary table</span><span class="mw-editsection"><span class="mw-editsection-bracket">[</span><a href="/wiki/index.php?title=Team:Glasgow/Parts_test&action=edit&section=1" title="Edit section: Summary table">edit</a><span class="mw-editsection-bracket">]</span></span></h2> |
| | + | <div id='groupparts' style='min-height:100px;width:700px;'><div style='width:300px;margin:2px;padding:20px;color:gray;border:1px solid gray'>Loading.....</div></div><script>$('#groupparts').load('/cgi/api/groupparts.cgi', { t:'iGEM015', g:'Glasgow' },function(){ $('#groupparts .tablesorter').tablesorter();} );</script> |
| | | | |
| − | });
| |
| − | </script>
| |
| | </body> | | </body> |
| | </html> | | </html> |
| | {{Glasgowfooter}} | | {{Glasgowfooter}} |