Difference between revisions of "Team:METU Turkey/Experiments"

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<h2 align=center>Protocols</h2>
 
<h2 align=center>Protocols</h2>
 
<p align=center>Standard iGEM <a class="one" href="http://parts.igem.org/Help" target="_blank">Protocols</a></p>
 
<p align=center>Standard iGEM <a class="one" href="http://parts.igem.org/Help" target="_blank">Protocols</a></p>
 +
<pre>
 +
Transformation Protocol:
 +
1)Throw competent cell on ice
 +
2) Mix ligation product 50 microliter cell+2 microliter plasmid
 +
3)Incubate cells on ice 30 mins
 +
4) Heat shock 55 sec CaCl2, 75 sec RuCl2
 +
Add 900 ml LB
 +
5)Incubate 37 celcius for 80 minute
 +
6)Centrifuge at 3000 po m 10 mın
 +
7)Discard supernatant
 +
8)Resuspend pellet in 100 ml LB
 +
9)Spread cells and wait 14-16 hours in the incubator
 +
</pre>
 
</div></br>
 
</div></br>
 
</tr>
 
</tr>

Revision as of 15:06, 15 September 2015

Welcome!
We are Team METU_Turkey!

Experiments

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Protocols

Standard iGEM Protocols

Transformation Protocol:
1)Throw competent cell on ice
2) Mix ligation product 50 microliter cell+2 microliter plasmid
3)Incubate cells on ice 30 mins
4) Heat shock 55 sec CaCl2, 75 sec RuCl2
Add 900 ml LB
5)Incubate 37 celcius for 80 minute
6)Centrifuge at 3000 po m 10 mın
7)Discard supernatant
8)Resuspend pellet in 100 ml LB
9)Spread cells and wait 14-16 hours in the incubator

Documentation of the development of our project

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Methodology

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