Difference between revisions of "Team:Cambridge-JIC/Autofocus"
KaterinaMN (Talk | contribs) |
KaterinaMN (Talk | contribs) |
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<li><p>Implemented the parabola approximation to find in focus position. </p></li> | <li><p>Implemented the parabola approximation to find in focus position. </p></li> | ||
</ul> | </ul> | ||
− | <p | + | <p>Gradient search, which is a simple technique for convergence to local extrema, was also attempted, but did not give satisfactory results, as the learning rate of the algorithm is slow. Also, noise from images drastically disrupts the calculations. This was a significant problem even when the gradient was calculated by taking multiple pictures around the point of interest. Another algorithm that we found was unsuccessful is the golden section interval algorithm.</p> |
<center><img src="//2015.igem.org/wiki/images/d/da/CamJIC-Software-Autofocus-Example.jpg" style="width:40%;margin:10px"><img src="//2015.igem.org/wiki/images/b/b1/CamJIC-Software-Autofocus-Graph.png" style="width:40%;margin:10px"><p><i>Autofocus algorithm in action: the plot shows the increase of the variance (i.e. the focus score) of the image with each iteration.</i></p></center> | <center><img src="//2015.igem.org/wiki/images/d/da/CamJIC-Software-Autofocus-Example.jpg" style="width:40%;margin:10px"><img src="//2015.igem.org/wiki/images/b/b1/CamJIC-Software-Autofocus-Graph.png" style="width:40%;margin:10px"><p><i>Autofocus algorithm in action: the plot shows the increase of the variance (i.e. the focus score) of the image with each iteration.</i></p></center> | ||
<p>The performance of the final autofocus algorithm varies, depending on the starting point of the search. A typical processing time is around 40s, which allows for autofocus during live-stream imaging through the <a href="" class="blue">Webshell</a>.</p> | <p>The performance of the final autofocus algorithm varies, depending on the starting point of the search. A typical processing time is around 40s, which allows for autofocus during live-stream imaging through the <a href="" class="blue">Webshell</a>.</p> |
Revision as of 23:11, 17 September 2015