Difference between revisions of "Team:UMBC-Maryland/Safety"

Latest revision as of 04:37, 18 September 2015

Safety in iGEM

Our chassis organism was E. Coli K12. We worked under sterile conditions using a flame and ethanol to sterilize the surfaces. All our plates/tubes were kept parafilmed and stored in a dedicated lab refrigerator/freezer. We clearly labeled all samples and were stored appropriately. We worked with Ethidium Bromide when staining our gels and made sure to gfollow handling and safety protocols. We wore nitrile gloves whenever handling organisms and deposited wastes in the appropriate containers. All flasks and tubes were autoclaved and bleached when finished. We wore appropriate lab attire and made sure to adhere to other lab safety guidelines.

This project can be used for copper remediation in the real world. If our project was applied in the real world, some risks involved would include the possibility of disturbing the natural habitat of other organisms, as well as creating an oxygen-limiting environment. We could include a mechanism to prevent extensive growth, or we could create a method to capture the cells after sufficient copper absorption.

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+<p> Our chassis organism was E. Coli K12. We worked under sterile conditions using a flame and ethanol to sterilize the surfaces. All our plates/tubes were kept parafilmed and stored in a dedicated lab refrigerator/freezer. We clearly labeled all samples and were stored appropriately. We worked with Ethidium Bromide when staining our gels and made sure to gfollow handling and safety protocols. We wore nitrile gloves whenever handling organisms and deposited wastes in the appropriate containers. All flasks and tubes were autoclaved and bleached when finished. We wore appropriate lab attire and made sure to adhere to other lab safety guidelines. </p>
+This project can be used for copper remediation in the real world. If our project was applied in the real world, some risks involved would include the possibility of disturbing the natural habitat of other organisms, as well as  creating an oxygen-limiting environment. We could include a mechanism to prevent extensive growth, or we could create a method to capture the cells after sufficient copper absorption.
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-<p> Our chassis organism was E. Coli K12. We worked under sterile conditions using a flame and ethanol to sterilize the surfaces. All our plates/tubes were kept parafilmed and stored in a dedicated lab refrigerator/freezer. We clearly labeled all samples and were stored appropriately. We worked with Ethidium Bromide when staining our gels and made sure to gfollow handling and safety protocols. We wore nitrile gloves whenever handling organisms and deposited wastes in the appropriate containers. All flasks and tubes were autoclaved and bleached when finished. We wore appropriate lab attire and made sure to adhere to other lab safety guidelines. </p>
-This project can be used for copper remediation in the real world. If our project was applied in the real world, some risks involved would include the possibility of disturbing the natural habitat of other organisms, as well as  creating an oxygen-limiting environment. We could include a mechanism to prevent extensive growth, or we could create a method to capture the cells after sufficient copper absorption.
-<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
-<h4>Safe Project Design</h4>
-<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
-<ul>
-<li>Choosing a non-pathogenic chassis</li>
-<li>Choosing parts that will not harm humans / animals / plants</li>
-<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
-<li>Including an "induced lethality" or "kill-switch" device</li>
-</ul>
-<h4>Safe Lab Work</h4>
-<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
-<h4>Safe Shipment</h4>
-<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
-</html>
-{{Team:UMBC-Maryland/TemplateBottom}}