Difference between revisions of "Team:BABS UNSW Australia/project/phlow"

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<h3>Acid shock response promoter</h3>
 
<h3>Acid shock response promoter</h3>
 
<p>An essential component of the pHlow plasmid is listeriolysin only being expressed once the cell in within the lysosome. Bacteria often are subjected to environmental stressors in the form of pH change, requiring differential gene expression, and hence have pH specific promoters. We have had these endogenous promoters synthesized and have characterised them for use in the pHlow plasmid.</p>
 
<p>An essential component of the pHlow plasmid is listeriolysin only being expressed once the cell in within the lysosome. Bacteria often are subjected to environmental stressors in the form of pH change, requiring differential gene expression, and hence have pH specific promoters. We have had these endogenous promoters synthesized and have characterised them for use in the pHlow plasmid.</p>
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<p>The acid shock response protein is present in the genome of E. Coli K12 and was first submitted into the registry by IIT Delhi 2013 (http://parts.igem.org/Part:BBa_K1170000) . This part was not available from the registry so we had it synthesised as a gBLOCK from IDT and biobricked it so that future years can access the part without synthesis.
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<p>The acid shock response protein is present in the genome of E. Coli K12 and was first submitted into the registry by IIT Delhi 2013 (<a href="http://parts.igem.org/Part:BBa_K1170000">http://parts.igem.org/Part:BBa_K1170000</a>) . This part was not available from the registry so we had it synthesised as a gBLOCK from IDT and biobricked it so that future years can access the part without synthesis.
 
</p>
 
</p>
  

Revision as of 04:46, 18 September 2015

Overview

The two proteins essential for bacterial invasion of mammalian cytoplasm, invasin and listeriolysin, were already in the registry, and were previously shown to work in a very simple genetic circuit - promoter-rbs-protein-rbs-protein. Our aim with the ‘pHlow’ system was to more finely tune the expression of these proteins, as well as improve the biosafety of modified organisms.

Acid shock response promoter

An essential component of the pHlow plasmid is listeriolysin only being expressed once the cell in within the lysosome. Bacteria often are subjected to environmental stressors in the form of pH change, requiring differential gene expression, and hence have pH specific promoters. We have had these endogenous promoters synthesized and have characterised them for use in the pHlow plasmid.

The acid shock response protein is present in the genome of E. Coli K12 and was first submitted into the registry by IIT Delhi 2013 (http://parts.igem.org/Part:BBa_K1170000) . This part was not available from the registry so we had it synthesised as a gBLOCK from IDT and biobricked it so that future years can access the part without synthesis.

Pseudoknots

wait are pseudoknots even going here or in a section of their own or both???