Team:BABS UNSW Australia/Protocols

Given the differing levels of experience in the team, and the plethora of protocols available on the net we decided to standardise our protocols and keep them in a tidy little orange folder. Here are most of the protocols we used over the year including our standard template.

Template

  • Babs UNSW iGEM protocol template

    • Media and Solutions

  • LB Broth Preparation
  • LB Agar Preparation
  • Agar Plate Preparation
  • Antibiotic Preparation
  • Streptococcal Regeneration Media
  • GM17 Media Preparation
  • SOC Media Preparation
  • Preparation of Tris-HCL Buffer
  • 1M Na2CO3

    • DNA Manipulation

  • Enzyme Digestion
  • Ligation iGEM protocol
  • Ligation iGEM protocol V2
  • Gel Electrophoresis

    • Transformations

  • E.coli Transformation
  • Preparation of Lactococcus Competent Cells
  • Lactococcus electrotransformation pre-treatement solution
  • Simple Lactococcus transformation
  • Lactococcus Transformation V1
  • Lactococcus Transformation V2

    • Other

  • Miniprep iGEM protocol
  • PCR purification iGEM protocol
  • Overlap PCR
  • Sequencing preparation iGEM Protocol
  • Mammalian Cell assay
  • LacZ Assay
  • SDS-PAGE protein gel