Team:BABS UNSW Australia/Parts
Lactoccocus Parts
- This protein-coding part encodes invasin tailored for use in Lactococcus species.The signal peptide region of Listeria monocytogenes was exchanged for a signal peptide from Lactobacillus plantarum. The aim of this is localise the invasin protein to the outer membrane when used in Lactococcus species. The LysM (lysine motif) protein domain embeds itself in the membrane, presenting the attached protein to the extracellular environment. The invasin gene was codon-optimised for expression in L. lactis
- This part is listeriolysin codon-optimised for expression in Lactococcus. By co-expressing with the above LysM-INV, Lactococcus species can enter into mammalian cells.
Pseudoknots
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BBa_K1677391: IBV Pseudoknot
- Sequence adapted from a pseudoknot sourced from the Infection Bronchitis Virus.
- Sequence adapted from a pseudoknot sourced from the Mouse Mammary Tumor Virus.
- Sequence adapted from a pseudoknot sourced from the Transmissible Gastroenteritis Virus.
- The above pseudoknots were submitted individually, for future use as translational regulatory mechanisms.
IPTG-inducible promoter + Pseudoknots
- The above three parts are composite biobricks composed of a promoter inducible by IPTG (BBa_J04500) followed by one pseudoknot. This device allows expression of a gene with a translational delay post-induction.
BBa_K1677800: IPTG inducible promoter with IBV pseudoknot
BBa_K1677801: IPTG inducible promoter with TGV pseudoknot
BBa_K1677802: IPTG inducible promoter with MMTV pseudoknot
pH promoters
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BBa_K1677300: Lactococcus lactis p170 promoter
- This promoter has enhanced activity under low pH.
Parts Table
<groupparts>iGEM015 BABS_UNSW_Australia</groupparts>