Difference between revisions of "Team:UMaryland/Results"

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<p style="font-size:64px"><b>Results:Hok/Sok</b>
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<p style="font-size:48px;text-align:center;font-family:Verdana, Geneva, sans-serif;"><b>Plating Tests</b>
  
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<h1 align="center"><b>Plating Tests</b></h1>
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<div style="float:left;width:450px;height:550px;">
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<h2><b>Negative Control (No Pressure)</b></h2>
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<p style="font-size:24px;text-align:left;font-family:Verdana, Geneva, sans-serif;">Negative Control (No Pressure)
 
<p><img src = "https://static.igem.org/mediawiki/2015/8/8c/UMDnegcontrolplate4.png"></p>
 
<p><img src = "https://static.igem.org/mediawiki/2015/8/8c/UMDnegcontrolplate4.png"></p>
 
<p style = "font-size:18px">Figure 1. Negative control was K1783002 (constitutive unstable RFP) grown in media without chloramphenicol. After 2-3 days, plates demonstrate fewer than 10 colonies per plate, suggesting that antibiotic resistance is now only minimally present in the culture.</p>
 
<p style = "font-size:18px">Figure 1. Negative control was K1783002 (constitutive unstable RFP) grown in media without chloramphenicol. After 2-3 days, plates demonstrate fewer than 10 colonies per plate, suggesting that antibiotic resistance is now only minimally present in the culture.</p>
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<h2><b>Chloramphenicol Pressure Only</b></h2>
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<p style="font-size:24px;text-align:left;font-family:Verdana, Geneva, sans-serif;">Chloramphenicol Pressure Only</b></h2>
 
<p><img src = "https://static.igem.org/mediawiki/2015/e/ec/UMDchlorplate3.png"></p>
 
<p><img src = "https://static.igem.org/mediawiki/2015/e/ec/UMDchlorplate3.png"></p>
 
<p style = "font-size:18px">Figure 2. K1783002 (constitutive unstable RFP) grown in media with chloramphenicol. Colonies persist through several days of plating, suggesting that antibiotic resistance was maintained throughout the test.</p>
 
<p style = "font-size:18px">Figure 2. K1783002 (constitutive unstable RFP) grown in media with chloramphenicol. Colonies persist through several days of plating, suggesting that antibiotic resistance was maintained throughout the test.</p>
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<h2><b>Chloramphenicol + Hok-Sok Pressure</b></h2>
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<p style="font-size:24px;text-align:left;font-family:Verdana, Geneva, sans-serif;">Chloramphenicol + Hok-Sok Pressure
 
<p><img src = "https://static.igem.org/mediawiki/2015/5/56/UMDhschlorplate2.png"></p>
 
<p><img src = "https://static.igem.org/mediawiki/2015/5/56/UMDhschlorplate2.png"></p>
 
<p style = "font-size:18px">Figure 3. K1783003 (constitutive unstable RFP under Hok-Sok regulation) grown in media with chloramphenicol. Colonies persist through several days of plating, suggesting that antibiotic resistance was maintained throughout the test.</p>
 
<p style = "font-size:18px">Figure 3. K1783003 (constitutive unstable RFP under Hok-Sok regulation) grown in media with chloramphenicol. Colonies persist through several days of plating, suggesting that antibiotic resistance was maintained throughout the test.</p>
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<h2><b>Hok-Sok Pressure Only</b></h2>
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<p style="font-size:24px;text-align:left;font-family:Verdana, Geneva, sans-serif;">Hok-Sok Pressure Only
 
<p><img src = "https://static.igem.org/mediawiki/2015/d/d0/UMDHSplate2.png"></p>
 
<p><img src = "https://static.igem.org/mediawiki/2015/d/d0/UMDHSplate2.png"></p>
 
<p style = "font-size:18px">Figure 4. K1783003 (constitutive unstable RFP under Hok-Sok regulation) grown in media without chloramphenicol. Colonies persist through several days of plating, suggesting that antibiotic resistance was maintained throughout the test.</p>
 
<p style = "font-size:18px">Figure 4. K1783003 (constitutive unstable RFP under Hok-Sok regulation) grown in media without chloramphenicol. Colonies persist through several days of plating, suggesting that antibiotic resistance was maintained throughout the test.</p>
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<h1 align="center"><b>Growth Curve</b></h1>
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<p style="font-size:48px;text-align:center;font-family:Verdana, Geneva, sans-serif;"><b>Growth Curve</b>
 
<p><img src = "https://static.igem.org/mediawiki/2015/3/37/UMDGrowthCurve2.png"></p>
 
<p><img src = "https://static.igem.org/mediawiki/2015/3/37/UMDGrowthCurve2.png"></p>
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<h1 align="center"><b>Fluorescence Studies</b></h1>
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<p style="font-size:32px;text-align:center;font-family:Verdana, Geneva, sans-serif;"><b>Fluorescence Studies</b>
  
 
<h1 align="center"><b>Analysis of Fluorescence Loss</b></h1>
 
<h1 align="center"><b>Analysis of Fluorescence Loss</b></h1>
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<p><img src= "https://static.igem.org/mediawiki/2015/thumb/9/92/UMaryland_Group_E_090615.JPG/800px-UMaryland_Group_E_090615.JPG"></p>
 
<p><img src= "https://static.igem.org/mediawiki/2015/thumb/9/92/UMaryland_Group_E_090615.JPG/800px-UMaryland_Group_E_090615.JPG"></p>
 
<p style = "font-size:18px">With or without added chloramphenicol, Hok-Sok appears to be able to maintain a relatively constant level of fluorescence.</p>
 
<p style = "font-size:18px">With or without added chloramphenicol, Hok-Sok appears to be able to maintain a relatively constant level of fluorescence.</p>
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<h1 align="center">Future Plans</h1>
 
  
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<h1 align = "center">Considerations</h1>
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<p style="font-size:32px;text-align:center;font-family:Verdana, Geneva, sans-serif;">Future Plans
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<p style="font-size:32px;text-align:center;font-family:Verdana, Geneva, sans-serif;">Considerations
 
<p style = "font-size:24px">PCR of Hok-Sok Construct</p>
 
<p style = "font-size:24px">PCR of Hok-Sok Construct</p>
 
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<p style = "font-size:18px">Due to the close proximity of the emission wavelength of RFP (584 nm) and the classical absorbance wavelength for measuring cell density (600 nm), it is difficult to accurately determine the cell density of cultures that are expressing RFP. Given more time to calibrate our testing measurements, we would either have used an alternative wavelength for measuring OD (>600 nm), used a hemocytometer as an alternate counting method, or switched to GFP as an alternative fluorescent marker whose emission wavelength differs from 600 nm by a greater amount.</p>
 
<p style = "font-size:18px">Due to the close proximity of the emission wavelength of RFP (584 nm) and the classical absorbance wavelength for measuring cell density (600 nm), it is difficult to accurately determine the cell density of cultures that are expressing RFP. Given more time to calibrate our testing measurements, we would either have used an alternative wavelength for measuring OD (>600 nm), used a hemocytometer as an alternate counting method, or switched to GFP as an alternative fluorescent marker whose emission wavelength differs from 600 nm by a greater amount.</p>
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<h2> Project Results</h2>
 
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<p>Here you can describe the results of your project and your future plans. </p>
 
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<h5>What should this page contain?</h5>
 
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<li> Clearly and objectively describe the results of your work.</li>
 
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<li> Future plans for the project </li>
 
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<li> Considerations for replicating the experiments </li>
 
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</ul>
 
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<h4> Project Achievements </h4>
 
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
 
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<li>A list of linked bullet points of the successful results during your project</li>
 
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
 
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<h4>Inspiration</h4>
 
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<p>See how other teams presented their results.</p>
 
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<ul>
 
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
 
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
 
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
 
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</ul>
 
  
 
</div>
 
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Revision as of 05:41, 18 September 2015