Difference between revisions of "Team:UMBC-Maryland/Notebook"

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<br>
 
<br>
 
<br> [[June 1st]]
 
<br> [[June 1st]]
−
<br> First meeting of the project over summer where we ran our PCR products on a 1% agarose gel with results shown in 6_1_15PCR.jpg. Band sizzes are determined from the ladder in N0467_thumb.gif.  
+
<br> First meeting of the project over summer where we ran our PCR products on a 1% agarose gel with results shown in 6_1_15PCR.jpg. Band sizes are determined from the ladder in N0467_thumb.gif.  
 
<br>
 
<br>
 
<br> [[June 4th]]
 
<br> [[June 4th]]
 
<br> iGEM team members attended a meeting with a professor and was given an overview to the procedure of 3A Assembly. We also discussed team structure and the CUP1 gene construct that is needed to be synthesized as a G-block. G-blocks are then submitted to IDT for sequencing.
 
<br> iGEM team members attended a meeting with a professor and was given an overview to the procedure of 3A Assembly. We also discussed team structure and the CUP1 gene construct that is needed to be synthesized as a G-block. G-blocks are then submitted to IDT for sequencing.
 +
<br>
 +
<br> '''Week 2'''
 
<br>
 
<br>
 
<br> [[June 8th]]
 
<br> [[June 8th]]
 
<br> Digest products with a combination of restriction enzymes (XbaI, HindIII, EcoRI)were ran on a 2% agarose gel. Both gel results came up inconclusive.
 
<br> Digest products with a combination of restriction enzymes (XbaI, HindIII, EcoRI)were ran on a 2% agarose gel. Both gel results came up inconclusive.
 +
<br>
 +
<br> '''Week 3'''
 +
<br>
 +
<br> [[June 15th]]
 +
<br> Repeated digestion to troubleshoot any possible errors that may have occurred during the protocol.
 +
<br>
 +
<br> '''Week 4'''
 +
<br>
 +
<br> [[June 22nd]]
 +
<br> Repeated analysis of the digestion products on the gel. Gel results showed no bands
 
<br>
 
<br>
 
<br> [[June 25th]]
 
<br> [[June 25th]]
 
<br> Mini-prep of the metallothionein plasmid.  
 
<br> Mini-prep of the metallothionein plasmid.  
 
<br>  
 
<br>  
 +
<br> '''Week 5'''
 +
<br>
 
<br> [[June 29th]]
 
<br> [[June 29th]]
−
<br> Digestion of the metallothionein plasmid with restriction enzymes (EcoRI & PstI). The digestion products were then run on a gel stained with ethidium bromide.  
+
<br> Digestion of the metallothionein plasmid with restriction enzymes (EcoRI & PstI). After further investigation, the Bulls-Eye solution was the cause of bands not appearing and we switched to Ethidium Bromide. The digestion products were then run on a gel stained with ethidium bromide. The gel depicted both digested and undigested bands that can be used to identify whether our restriction enzymes are working or not.  
 
<p> Document the dates you worked on your project.</p>
 
<p> Document the dates you worked on your project.</p>
  

Revision as of 15:49, 2 July 2015



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Notebook

'''Week 1'''

[[June 1st]]
First meeting of the project over summer where we ran our PCR products on a 1% agarose gel with results shown in 6_1_15PCR.jpg. Band sizes are determined from the ladder in N0467_thumb.gif.

[[June 4th]]
iGEM team members attended a meeting with a professor and was given an overview to the procedure of 3A Assembly. We also discussed team structure and the CUP1 gene construct that is needed to be synthesized as a G-block. G-blocks are then submitted to IDT for sequencing.

'''Week 2'''

[[June 8th]]
Digest products with a combination of restriction enzymes (XbaI, HindIII, EcoRI)were ran on a 2% agarose gel. Both gel results came up inconclusive.

'''Week 3'''

[[June 15th]]
Repeated digestion to troubleshoot any possible errors that may have occurred during the protocol.

'''Week 4'''

[[June 22nd]]
Repeated analysis of the digestion products on the gel. Gel results showed no bands

[[June 25th]]
Mini-prep of the metallothionein plasmid.

'''Week 5'''

[[June 29th]]
Digestion of the metallothionein plasmid with restriction enzymes (EcoRI & PstI). After further investigation, the Bulls-Eye solution was the cause of bands not appearing and we switched to Ethidium Bromide. The digestion products were then run on a gel stained with ethidium bromide. The gel depicted both digested and undigested bands that can be used to identify whether our restriction enzymes are working or not.

Document the dates you worked on your project.

What should this page have?
  • Chronological notes of what your team is doing.
  • Brief descriptions of daily important events.
  • Pictures of your progress.
  • Mention who participated in what task.

Inspiration

You can see what others teams have done to organize their notes: