Difference between revisions of "Team:UMaryland/Practices"
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<p style="font-size:32px;text-align:center;font-family:Verdana, Geneva, sans-serif;">Grand View Farms | <p style="font-size:32px;text-align:center;font-family:Verdana, Geneva, sans-serif;">Grand View Farms | ||
<p style="font-size:18px;text-align:justify;">As our project deals with alternatives to antibiotics as plasmid maintenance, we wanted to learn how and why others avoid using antibiotics in their industries. According to a <a href="http://www.fda.gov/downloads/ForIndustry/UserFees/AnimalDrugUserFeeActADUFA/UCM440584.pdf">2013 Summary Report by the FDA </a> on antimicrobial use, a large majority of antimicrobials in the us are used in food-producing animals. Because of this we decided to meet directly with farmers who choose to purposely avoid antibiotics. In addition to learning about their rationale for using alternatives, these trips also helped inform us of the potential applications of our work with the Hok-Sok system.</p> | <p style="font-size:18px;text-align:justify;">As our project deals with alternatives to antibiotics as plasmid maintenance, we wanted to learn how and why others avoid using antibiotics in their industries. According to a <a href="http://www.fda.gov/downloads/ForIndustry/UserFees/AnimalDrugUserFeeActADUFA/UCM440584.pdf">2013 Summary Report by the FDA </a> on antimicrobial use, a large majority of antimicrobials in the us are used in food-producing animals. Because of this we decided to meet directly with farmers who choose to purposely avoid antibiotics. In addition to learning about their rationale for using alternatives, these trips also helped inform us of the potential applications of our work with the Hok-Sok system.</p> | ||
| − | <p style="font-size:18px;text-align:justify;"> Grand View Farms is a certified organic farm in Maryland that does not administer sub-therapeutic doses of antibiotics to their animals, uses no pesticides, and grows as few genetically modified crops as possible. As different animals consume different plants, they argued, rotating livestock through the land naturally maintains a healthy nutrient balance while suppressing the growth of harmful weeds. Due to this practice, the farmers at Grand View argued that administering antibiotics was not beneficial, since they naturally received all the nutrients required for good health and from the varied vegetation available for grazing.</p> | + | <p style="font-size:18px;text-align:justify;"> <a href="http://www.grandviewfarming.com/">Grand View Farms</a> is a certified organic farm in Maryland that does not administer sub-therapeutic doses of antibiotics to their animals, uses no pesticides, and grows as few genetically modified crops as possible. As different animals consume different plants, they argued, rotating livestock through the land naturally maintains a healthy nutrient balance while suppressing the growth of harmful weeds. Due to this practice, the farmers at Grand View argued that administering antibiotics was not beneficial, since they naturally received all the nutrients required for good health and from the varied vegetation available for grazing.</p> |
<p style="font-size:18px;text-align:justify;">Interestingly, the owners of Grand View Farms did not express any particular ethical or health concerns with GMO crops. Instead they informed us that, for a farm of their size, having an organic label made them more profitable and allows them to fill a market niche without directly competing with larger, more industrial scale operations. The allure of organically grown food and organically raised animals is a powerful marketing tool for the farm.</p> | <p style="font-size:18px;text-align:justify;">Interestingly, the owners of Grand View Farms did not express any particular ethical or health concerns with GMO crops. Instead they informed us that, for a farm of their size, having an organic label made them more profitable and allows them to fill a market niche without directly competing with larger, more industrial scale operations. The allure of organically grown food and organically raised animals is a powerful marketing tool for the farm.</p> | ||
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| − | <p style = "font-size:18px;text-align:justify";>The farmers' willingness to accept GMO crops demonstrated their interest in maintaining an antibiotic free operation. This helped us gain a greater understanding of the applications for our Hok-Sok plasmid maintenance system. If this could be used to maintain plasmids in lieu of antibiotics, then synthetic biology could benefit and partner with organizations like Grand View Farms without compromising the integrity of their | + | <p style = "font-size:18px;text-align:justify";>The farmers' willingness to accept GMO crops demonstrated their interest in maintaining an antibiotic free operation. This helped us gain a greater understanding of the applications for our Hok-Sok plasmid maintenance system. If this could be used to maintain plasmids in lieu of antibiotics, then synthetic biology could benefit and partner with organizations like Grand View Farms without compromising the integrity of their ethical positions. Genetically modified bacteria which increase soil quality or the nutrient uptake of animals when ingested are just two examples of how synthetic biology could benefit farms, but the prior necessity of antibiotics prohibited their acceptance at places like Grand View. However, we knew that without antibiotics, plasmids could not be maintained within the cell for very long, leading to a highly variable level of expression. We thus made sure to ensure that our Hok-Sok system could not only maintain plasmids, but also ensure a consistent expression level.</p> |
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<p style="font-size:24px;text-align:justify;"> <b>Baltimore, Maryland</b> | <p style="font-size:24px;text-align:justify;"> <b>Baltimore, Maryland</b> | ||
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| − | <p style="font-size:18px;text-align:justify;">We met with a researcher who studies the causes of AMD, Dr. Noriko Esumi. She currently studies retinal pigment epithelium (RPE) and how it relates to AMD. After giving her an explanation of what iGEM is and why we were choosing to study lutein production, she told us that, while her experience with lutein itself was limited, she was well informed about retinal health and some of the causes of macular degeneration. | + | <p style="font-size:18px;text-align:justify;">We met with a researcher who studies the causes of AMD, Dr. Noriko Esumi. She currently studies the retinal pigment epithelium (RPE) and how it relates to AMD. After giving her an explanation of what iGEM is and why we were choosing to study lutein production, she told us that, while her experience with lutein itself was limited, she was well informed about retinal health and some of the causes of macular degeneration. |
From our conversation, we learned that age-related macular degeneration can be attributed to a few factors: smoking, immune responses based on bacterial infections, genetics, and, as its name suggests, aging. She also supported the notion that lutein is an effective vitamin supplement to help maintain and improve ocular health. | From our conversation, we learned that age-related macular degeneration can be attributed to a few factors: smoking, immune responses based on bacterial infections, genetics, and, as its name suggests, aging. She also supported the notion that lutein is an effective vitamin supplement to help maintain and improve ocular health. | ||
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<h1 style="text-align:justify;"><b>Bioprocess Scale-up Facility</b></h1> | <h1 style="text-align:justify;"><b>Bioprocess Scale-up Facility</b></h1> | ||
| − | <p style="font-size:18px;text-align:justify;">As part of our side project on the biosynthesis of lutein and to acquire a better understanding of the challenges facing synthetic biology entrepreneurs in moving their project from a proof of concept to an industrial level we visited the University of Maryland Bioprocess Scale-up Facility. BSF is a facility at the University of Maryland provides training and is contracted by biotechnology companies in the region to optimize and | + | <p style="font-size:18px;text-align:justify;">As part of our side project on the biosynthesis of lutein and to acquire a better understanding of the challenges facing synthetic biology entrepreneurs in moving their project from a proof of concept to an industrial level we visited the University of Maryland <a href="http://www.brep.umd.edu/bsf.html">Bioprocess Scale-up Facility </a>. BSF is a facility at the University of Maryland which provides training and is contracted by biotechnology companies in the region to optimize and prove scale up of their biosynthesis and fermentation processes to the industrial level. They kindly allowed us to tour the facility and learn about some of the unique challenges involved in reaching industrial size in a bioprocess. Gaining a greater appreciation for this both informed and invigorated our goal of making synthetic biology cheaper, safer, and more affordable for community DIY labs and entrepreneurs.</p> |
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Revision as of 13:44, 18 September 2015
