Difference between revisions of "Team:UMaryland/Part Collection"

 
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/*____________________________________Parts____________________________________*/
  
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#biobricks
 
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/*____________________________________Parts____________________________________*/
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</style>
 
</style>
  
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<div id='cover'>
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</div>
  
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<div id='bar'>
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<p style="font-size:48px"><b>All Parts</b></style>
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<p style="font-size:24px">All information on our parts can be found on this page
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<div id='buttonset'>
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<a href="https://2015.igem.org/Team:UMaryland/Basic_Part">
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<div id='button'>
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<p> Basic Parts
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</div>
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</a>
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<a href="https://2015.igem.org/Team:UMaryland/Composite_Part">
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<div id='button'>
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<p>Composite Parts
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</div>
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</a>
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</div>
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<p style="font-size:18px">We've also included links to these side pages where we seperate our basic and composite parts
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</div>
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</div>
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</div>
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</div>
  
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<h1>Parts List</h1>
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<div id='layer2'>
  
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<table id="biobricks">
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<p style="font-size:36px;text-align:center;"><b>Full Parts List</b></h1>
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<div id='biobricks1'>
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<table id="biobricks" style="text-align:center;">
 
<tr>
 
<tr>
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  <th>Name</th>
 
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  <th>Info</th>
 
 
   <th>Registry</th>
 
   <th>Registry</th>
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  <th>Name</th>
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  <th>Function</th>
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  <th>Part Type</th>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
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<td>Miraculin</td>
 
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<td>Makes miraculin</td>
 
 
<td><a href="http://parts.igem.org/wiki/index.php/Part:BBa_K1783000">K1783000</a></td>
 
<td><a href="http://parts.igem.org/wiki/index.php/Part:BBa_K1783000">K1783000</a></td>
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<td>pBAD-RBS-Tagged-Miraculin</td>
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<td>Makes miraculin containing a transport tag for proper folding and a his-tag for purification</td>
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<td><a href="https://2015.igem.org/Team:UMaryland/Composite_Part#000">Composite</a></td>
 
</tr>
 
</tr>
 
<tr class="alt">
 
<tr class="alt">
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<td>Hok/Sok</td>
 
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<td>Toxin/antitoxin system</td>
 
 
<td><a href="http://parts.igem.org/Part:BBa_K1783001">K1783001</a></td>
 
<td><a href="http://parts.igem.org/Part:BBa_K1783001">K1783001</a></td>
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<td>Hok/Sok</td>
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<td>Type I Toxin/antitoxin (TA) system, naturally used to maintain plasmids</td>
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<td><a href="https://2015.igem.org/Team:UMaryland/Composite_Part#001">Composite</a></td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
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<td>QD RFP</td>
 
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<td>RFP with half life of 1 hour, promotor+rbs included</td>
 
 
<td><a href="http://parts.igem.org/Part:BBa_K1783002">K1783002</a></td>
 
<td><a href="http://parts.igem.org/Part:BBa_K1783002">K1783002</a></td>
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<td>Constitutive unstable RFP</td>
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<td>RFP under a constitutive promoter, with half life of 1 hour</td>
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<td><a href="https://2015.igem.org/Team:UMaryland/Basic_Part#002">Basic</a></td>
 
</tr>
 
</tr>
 
<tr class="alt">
 
<tr class="alt">
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<td>H/S+RFP</td>
 
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<td>Used to test retention of Hok/Sok system</td>
 
 
<td><a href="http://parts.igem.org/Part:BBa_K1783003">K1783003</a></td>
 
<td><a href="http://parts.igem.org/Part:BBa_K1783003">K1783003</a></td>
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<td>Hok-Sok+CU-RFP</td>
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<td>Constitutive unstable RFP, under Hok-Sok regulation. Used as part of tests of Hok-Sok's ability to retain plasmids</td>
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<td><a href="https://2015.igem.org/Team:UMaryland/Composite_Part#003">Composite</a></td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K1783004">K1783004</a></td>
 
<td>&epsilon;-cyclase</td>
 
<td>&epsilon;-cyclase</td>
 
<td>Converts lycopene into &alpha;-carotene</td>
 
<td>Converts lycopene into &alpha;-carotene</td>
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<td><a href="http://parts.igem.org/Part:BBa_K1783004">K1783004</a></td>
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<td><a href="https://2015.igem.org/Team:UMaryland/Basic_Part#004">Basic</a></td>
 
</tr>
 
</tr>
 
<tr class="alt">
 
<tr class="alt">
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<td><a href="http://parts.igem.org/Part:BBa_K1783005">K1783005</a></td>
 
<td>&epsilon;-hydroxylase</td>
 
<td>&epsilon;-hydroxylase</td>
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<td>Adds OH group to &epsilon; ring</td>
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<td>Adds hydroxyl group to &epsilon; ring</td>
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<td><a href="http://parts.igem.org/Part:BBa_K1783005">K1783005</a></td>
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<td><a href="https://2015.igem.org/Team:UMaryland/Basic_Part#005">Basic</a></td>
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</tr>
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<tr>
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<td>Gene</td>
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<td>???</td>
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<td><a href="#">???</a></td>
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</tr>
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<tr class="alt">
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<td>Gene</td>
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<td>???</td>
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<td><a href="#">???</a></td>
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</tr>
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−
<tr>
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<td>Gene</td>
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<td>???</td>
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<td><a href="#">???</a></td>
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</tr>
 
</tr>
 
</table>
 
</table>
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<hr>
 
  
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</div>
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</div>
  
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<style type="text/css">
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#partcontainer {
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overflow:hidden;
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width:1000px;
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<div id='layer1'>
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<div id='partcontainer'>
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<div id='partname'>
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<h2>Miraculin Generator</h2>
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</div>
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<div id='partdescription'>
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<p style = "font-size:18px">Combines <a href = "http://parts.igem.org/Part:BBa_K206000">BBa_K206000</a> (pBAD promoter) with <a href = "http://parts.igem.org/Part:BBa_K1033120">BBa_K1033120</a> (B0034-ExpTag-Linker-Miraculin-Linker-6xHis). Codes for a His-tagged miraculin with export tag to periplasm. Induced with arabinose. Miraculin is a protein that interacts with our taste receptors, causing us to taste sour foods as sweet. This part was assembled using 3A Assembly as a training exercise for new UMaryland iGEM members.</p>
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</div>
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<div id='partimage'>
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<a href = "http://parts.igem.org/Part:BBa_K1783000"><img src="https://static.igem.org/mediawiki/2015/5/51/000.jpg" height="337" width="457"></a>
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</div>
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</div>
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<br>
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<br>
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<div id='partcontainer'>
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<div id='partname'>
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<h2>Hok/Sok Type I Toxin-Antitoxin Cassette</h2>
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</div>
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<div id='partdescription'>
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<p style = "font-size:18px">Codes for the Hok-Sok Type I toxin-antitoxin system. This system naturally evolved in bacteria as a method to maintain plasmids. Hok-Sok is noted for its presence on the R1 plasmid, which contains genes that confer antibiotic resistance. Hok-Sok is believed to be used to maintain these plasmids in the absence of antibiotic.</p>
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</div>
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<div id='partimage'>
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<a href = "http://parts.igem.org/Part:BBa_K1783001"><img src="https://static.igem.org/mediawiki/2015/a/a0/001.jpg" height="337" width="457"></a>
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</div>
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</div>
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<br>
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<br>
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<div id='partcontainer'>
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<div id='partname'>
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<h2>Constitutive Unstable RFP</h2>
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</div>
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<div id='partdescription'>
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<p style = "font-size:18px">Complete with a constitutive promoter, this fluorescent protein is expressed continuously in the cytoplasm. Due to the presence of a LVA degradation tag, this protein is subject to rapid targeting via proteases, with a half life of about 1 hour.</p>
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</div>
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<div id='partimage'>
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<a href = "http://parts.igem.org/Part:BBa_K1783002"><img src="https://static.igem.org/mediawiki/2015/1/11/002.jpg" height="337" width="457"></a>
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</div>
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</div>
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<br>
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<br>
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<div id='partcontainer'>
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<div id='partname'>
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<h2>Constitutive Unstable RFP under Hok-Sok Regulation</h2>
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</div>
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<div id='partdescription'>
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<p style = "font-size:18px">This part combines two of our previous parts: Hok-Sok TA cassette and Constitutive Unstable RFP. This testing device was used to measure the ability of the Hok-Sok maintenance system to force cells to retain plasmids. The presence of Hok-Sok adjacent to the constitutive promoter for unstable RFP was found to interfere with RFP production in a minor way.</p>
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</div>
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<div id='partimage'>
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<a href = "http://parts.igem.org/Part:BBa_K1783003"><img src="https://static.igem.org/mediawiki/2015/9/9c/003.jpg" height="337" width="457"></a>
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</div>
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</div>
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<br>
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<br>
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<div id='partcontainer'>
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<div id='partname'>
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<h2>Epsilon Cyclase</h2>
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</div>
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<div id='partdescription'>
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<p style = "font-size:18px">Epsilon cyclase from the <i>A. thaliana</i> genome is capable of converting lycopene to gamma-carotene. A transport tag for the chloroplast at the N-terminus has been removed and the coding sequence has been codon-optimized for <i>E. coli</i>.</p>
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</div>
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<div id='partimage'>
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<a href = "http://parts.igem.org/Part:BBa_K1783004"><img src="https://static.igem.org/mediawiki/2015/thumb/2/20/K1783004.png/800px-K1783004.png" height="337" width="457"></a>
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</div>
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</div>
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<br>
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<br>
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<div id='partcontainer'>
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<div id='partname'>
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<h2>Epsilon Hydroxylase</h2>
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</div>
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<div id='partdescription'>
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<p style = "font-size:18px">Epsilon cyclase from the <i>A. thaliana</i> genome is capable of converting alpha-carotene to alpha-cryptoxanthin. A transport tag for the chloroplast at the N-terminus has been removed and the coding sequence has been codon-optimized for <i>E. coli</i>.</p>
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</div>
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<div id='partimage'>
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<a href = "http://parts.igem.org/Part:BBa_K1783005"><img src="https://static.igem.org/mediawiki/2015/thumb/0/0d/K1783005.png/800px-K1783005.png" height="337" width="457"></a>
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</div>
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<br>
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<br>
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Latest revision as of 01:09, 19 September 2015