Difference between revisions of "Team:UMaryland/Composite Part"

 
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<h2> Composite Parts</h2>
 
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<p style="font-size:48px"><b>Composite Parts</b></style>
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    <div class = "name"> <b> Miraculin Generator </b> </div>
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<p style="font-size:24px">This page contains just the composite parts we made. Click below to see all our parts.
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      <div class = "catalog"> K1783000 </div>
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<div id='buttonset'>
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        <h3> Miraculin Gene with pBAD promoter
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<a href="https://2015.igem.org/Team:UMaryland/Part_Collection">
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        </h3>
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<div id='button'>
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          <p>
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<p> All Parts
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          pBAD-B0034-ExpTag-Linker-Miraculin-Linker-6xHis. Adds a promoter to BBa_K1033120 in order to characterize it. Under arabinose induction, produces miraculin connected to an export tag and a His-tag via unstructured linkers. Miraculin is a taste modifying protein.
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</div>
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          </p>
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</a>
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      </div>
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<p style="font-size:18px">A composite part is a biobrick made from simpler biobricks,
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    </div>
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    </div>
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</div>
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</div>
 
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<div id='partname'>
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    <div class = "name"> <b> Unstable RFP under Hok-Sok Regulation </b> </div>
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<h2>Miraculin Generator</h2>
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      <div class = "catalog"> K1783000 </div>
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      <div class = desc>
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        <h3> Hok-Sok gene linked to unstable RFP
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        </h3>
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          <p>
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          Produces unstable RFP under a constitutive promoter. This construct carries the hok-sok toxin-antitoxin (TA) plasmid maintenance system and was used to measure the ability of this alternative plasmid maintenance system to keep plasmids within the cell without the use of antibiotics.
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          </p>
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      </div>
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    </div>
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    </div>
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<p style = "font-size:18px">Combines <a href = "http://parts.igem.org/Part:BBa_K206000">BBa_K206000</a> (pBAD promoter) with <a href = "http://parts.igem.org/Part:BBa_K1033120">BBa_K1033120</a> (B0034-ExpTag-Linker-Miraculin-Linker-6xHis). Codes for a His-tagged miraculin with export tag to periplasm. Induced with arabinose. Miraculin is a protein that interacts with our taste receptors, causing us to taste sour foods as sweet. This part was assembled using 3A Assembly as a training exercise for new UMaryland iGEM members.</p>
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</div>
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<div id='partimage'>
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<a href = "http://parts.igem.org/Part:BBa_K1783000"><img src="https://static.igem.org/mediawiki/2015/5/51/000.jpg" height="337" width="457"></a>
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</div>
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<h2>Constitutive Unstable RFP</h2>
 
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<p style = "font-size:18px">Complete with a constitutive promoter, this fluorescent protein is expressed continuously in the cytoplasm. Due to the presence of a LVA degradation tag, this protein is subject to rapid targeting via proteases, with a half life of about 1 hour.</p>
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<a href = "http://parts.igem.org/Part:BBa_K1783002"><img src="https://static.igem.org/mediawiki/2015/1/11/002.jpg" height="337" width="457"></a>
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<h4>Note</h4>
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<h2>Constitutive Unstable RFP under Hok-Sok Regulation</h2>
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<p>In order to be considered for the <a href="https://2015.igem.org/Judging/Awards#SpecialPrizes">Best New Composite Part award</a>, you must fill out this page. Please give links to the Registry entries for the Composite parts you have made. Please see the Registry's <a href="http://parts.igem.org/Help:Parts#Basic_and_Composite_Parts"> Help:Parts page</a> for more information on part types.</p>
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<p style = "font-size:18px">This part combines two of our previous parts: Hok-Sok TA cassette and Constitutive Unstable RFP. This testing device was used to measure the ability of the Hok-Sok maintenance system to force cells to retain plasmids. The presence of Hok-Sok adjacent to the constitutive promoter for unstable RFP was found to interfere with RFP production in a minor way.</p>
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</div>
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<div id='partimage'>
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<a href = "http://parts.igem.org/Part:BBa_K1783003"><img src="https://static.igem.org/mediawiki/2015/9/9c/003.jpg" height="337" width="457"></a>
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A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
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<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
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Latest revision as of 01:13, 19 September 2015