Difference between revisions of "Team:UMBC-Maryland/Results"

 
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<h2> Project Results</h2>
  
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We genetically engineered several strains of E. coli in an effort to increase copper resistance and collection. We named each strain after our school's mascot, the Chesapeake Bay Retriever. Retriever 1 (Ret1) has been given the yeast metallothionein gene CUP1. Retriever 2 (Ret2) has been given the same CUP1 gene codon optimized for E. coli. Retriever 3 (Ret3) has been given the CUP1 gene codon optimized, fused with bacteriophage lambda receptor (LamB). Each strain was tested for cell density and extracellular copper concentration at different initial copper sulfate concentrations for 7-10 hours.
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Ret1 and Ret3 showed no significant cell growth, possibly due to the toxicity of the protein, or due to the stress placed on the cell from protein synthesis.
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The Ret1 strain showed no significant difference from control in either copper uptake or cell viability in copper. The Ret2 strain showed increased cell viability in high copper concentrations, but did not show any significant increase in copper uptake ability.  The Ret3 strain showed very low growth most likely due to excessive protein load.  However, it showed a marked increase in copper uptake as compared to the control strain.
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In the future, we would like to adjust the expression of the LamB metallothionein fusion in order to increase the viability of the cell line.  Eventually, we would like to insert a gasification gene in order to allow for the easy collection of copper laden E. coli.
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<a href="https://2015.igem.org/Team:UMBC-Maryland"><li>HOME</li></a>
 
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<a href="https://2015.igem.org/Team:UMBC-Maryland/Team"><li>TEAM</li></a>
 
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<a href="#"><li>PROJECT
 
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<a href="https://2015.igem.org/Team:UMBC-Maryland/Description"><li>Description</li></a>
 
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<a href="https://2015.igem.org/Team:UMBC-Maryland/Experiments"><li>Experiments & Protocols</li></a> 
 
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<a href="https://2015.igem.org/Team:UMBC-Maryland/Results"><li>Results</li></a> 
 
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<a href="#"><li>PARTS
 
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<a href="https://2015.igem.org/Team:UMBC-Maryland/Parts"><li>Team Parts</li></a>
 
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<a href="https://2015.igem.org/Team:UMBC-Maryland/Basic_Part"><li>Basic Parts</li></a> 
 
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<a href="https://2015.igem.org/Team:UMBC-Maryland/Composite_Part"><li>Composite Parts</li></a>
 
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<a href="https://2015.igem.org/Team:UMBC-Maryland/Part_Collection"><li>Part Collection</li></a> 
 
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<a href="https://2015.igem.org/Team:UMBC-Maryland/Attributions"><li>ATTRIBUTIONS</li></a>
 
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<a href="https://2015.igem.org/Team:UMBC-Maryland/Safety"><li>SAFETY</li></a>
 
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[[File:UMBCRet1OD.jpg|700px]]
 
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<h2> Project Results</h2>
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We genetially engienered several strains of E. coli in an effort to increase copper resistance and collection. We named each strain after our school's mascot, the Chesapeake Bay Retriever. Retriever 1 (Ret1) has been given the yeast metallothionein gene CUP1. Retriever 2 (Ret2) has been given the same CUP1 gene codon optimized for E. coli.
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[[File:UMBCCu8_19_15.jpg]]
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<p>Here you can describe the results of your project and your future plans. </p>
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<h5>What should this page contain?</h5>
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<li> Clearly and objectively describe the results of your work.</li>
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<li> Future plans for the project </li>
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<li> Considerations for replicating the experiments </li>
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<h4> Project Achievements </h4>
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[[File:UMBCRet3OD.jpg|700px]]
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
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<li>A list of linked bullet points of the successful results during your project</li>
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[[File:UMBCRet3Cu.jpg|500px]]
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
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<h4>Inspiration</h4>
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<p>See how other teams presented their results.</p>
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
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Latest revision as of 02:52, 19 September 2015

Project Results

We genetically engineered several strains of E. coli in an effort to increase copper resistance and collection. We named each strain after our school's mascot, the Chesapeake Bay Retriever. Retriever 1 (Ret1) has been given the yeast metallothionein gene CUP1. Retriever 2 (Ret2) has been given the same CUP1 gene codon optimized for E. coli. Retriever 3 (Ret3) has been given the CUP1 gene codon optimized, fused with bacteriophage lambda receptor (LamB). Each strain was tested for cell density and extracellular copper concentration at different initial copper sulfate concentrations for 7-10 hours.

Ret1 and Ret3 showed no significant cell growth, possibly due to the toxicity of the protein, or due to the stress placed on the cell from protein synthesis. The Ret1 strain showed no significant difference from control in either copper uptake or cell viability in copper. The Ret2 strain showed increased cell viability in high copper concentrations, but did not show any significant increase in copper uptake ability. The Ret3 strain showed very low growth most likely due to excessive protein load. However, it showed a marked increase in copper uptake as compared to the control strain.

In the future, we would like to adjust the expression of the LamB metallothionein fusion in order to increase the viability of the cell line. Eventually, we would like to insert a gasification gene in order to allow for the easy collection of copper laden E. coli.

UMBCRet1OD.jpg

UMBCRet1Cu.jpg

UMBCRet2OD.jpg

UMBCRet2Cu.jpg

UMBCRet3OD.jpg

UMBCRet3Cu.jpg