Difference between revisions of "Team:METU Turkey/Results"

 
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<body>
 
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        <li>
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            <a class="one" href="https://2015.igem.org/Team:METU_Turkey">Home</a>
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<td id="rcorners1" align=center > <a class="one" href="https://2015.igem.org/Team:METU_Turkey">HOME</a></td>
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         </li>
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<td id="rcorners1" align=center> <a class="one" href="https://2015.igem.org/Team:METU_Turkey/Team">TEAM</a> </td>
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<td id="rcorners1" align=center><a class="one" href="https://2015.igem.org/Team:METU_Turkey/Project">PROJECT</a></td>
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            <a class="one" href="https://2015.igem.org/Team:METU_Turkey/Team">Team</a>
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<td id="rcorners1" align=center><a class="one" href="https://2015.igem.org/Team:METU_Turkey/Parts">PARTS</a></td>
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<td id="rcorners1" align=center><a class="one" href="https://2015.igem.org/Team:METU_Turkey/Notebook">NOTEBOOK</a></td>
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        </li>
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<td id="rcorners1" align=center><a class="one" href="https://2015.igem.org/Team:METU_Turkey/Attributions">ATTRIBUTIONS</a></td>
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                <li>
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<td id="rcorners1" align=center><a class="one" href="https://2015.igem.org/Team:METU_Turkey/interlab">INTERLAB</a></td>
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            <a class="one" href="https://2015.igem.org/Team:METU_Turkey/Description">Project</a>
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            <ul>
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                <li><a href="https://2015.igem.org/Team:METU_Turkey/Experiments">Experiments</a></li>
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                <li><a href="https://2015.igem.org/Team:METU_Turkey/Results">Results</a></li>
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                <li><a href="https://2015.igem.org/Team:METU_Turkey/Design">Design</a></li>
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            </ul>
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        </li>
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        <li>
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            <a class="one" href="https://2015.igem.org/Team:METU_Turkey/Parts">Parts</a>
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            <ul>
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                <li><a href="https://2015.igem.org/Team:METU_Turkey/Basic_Part">Basic Part</a></li>
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                <li><a href="https://2015.igem.org/Team:METU_Turkey/Composite_Part">Composite Part</a></li>
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                <li><a href="https://2015.igem.org/Team:METU_Turkey/Part_Collection">Part Collection</a></li>
 +
            </ul>
 +
            </li>
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        <li>
 +
            <a class="one" href="https://2015.igem.org/Team:METU_Turkey/Notebook">Notebook</a>
 +
        </li>
 +
        <li>
 +
            <a class="one" href="https://2015.igem.org/Team:METU_Turkey/Attributions">Attributions</a>
 +
        </li>
 +
        <li>
 +
            <a class="one" href="https://2015.igem.org/Team:METU_Turkey/interlab">Interlab</a>
 +
        </li>
 +
        <li>
 +
            <a class="one" href="https://2015.igem.org/Team:METU_Turkey/Collaborations">Collaborations</a>
 +
        </li>
 +
        <li>
 +
            <a class="one" href="https://2015.igem.org/Team:METU_Turkey/Practices">Human Practices</a>
 +
        </li>
 +
        <li>
 +
            <a class="one" href="https://2015.igem.org/Team:METU_Turkey/Safety">Safety</a>
 +
        </li>
 +
        <li>
 +
            <a class="one" href="https://2015.igem.org/Team:METU_Turkey/Gallery">Gallery</a>
 +
        </li>
 +
        <li>
 +
            <a class="one" href="https://2015.igem.org/Team:METU_Turkey/Sponsors">Sponsors</a>
 +
        </li>
 +
   
 +
        </li>
 +
    </ul>
 +
 
 +
<!-- First block of content -->
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<table  cellspacing="20px" height="150px" align="center">
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<tr>
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<td align="center" width="350px"><a href="https://igem.org/Main_Page" target="_blank"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="150px"></a></td>
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<td align="center" bgColor="#FFFFFF" >
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<!-- You can replace this image with your team's logo! -->
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<img src="https://static.igem.org/mediawiki/2014/c/cc/Metuigem_logo.png" width="120px">
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<h3>Welcome!  <br> We are Team METU_Turkey! </h3>
 +
 
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</td>
 +
<td align="center" width="162px"><a href="http://www.metu.edu.tr"color:#FFFFFF" target="_blank"><img src="https://static.igem.org/mediawiki/2014/9/91/Metulogo.jpg" width="300px"></a></td>
 
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<td id="rcorners1" align=center><a class="one" href="https://2015.igem.org/Team:METU_Turkey/Collaborations">COLLABORATIONS</a></td>
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<td id="rcorners1" align=center><a class="one" href="https://2015.igem.org/Team:METU_Turkey/Practices">HUMAN PRACTICES</a></td>
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<td id="rcorners1" align=center><a class="one" href="https://2015.igem.org/Team:METU_Turkey/Safety">SAFETY</a></td>
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<td id="rcorners1" align=center><a class="one" href="https://2015.igem.org/Team:METU_Turkey/Modeling">MODELING</a></td>
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<td id="rcorners1" align=center><a class="one" href="https://2015.igem.org/Team:METU_Turkey/Measurement">MEASUREMENT</a></td>
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<td id="rcorners1" align=center><a class="one" href="https://2015.igem.org/Team:METU_Turkey/Galery">GALERY</a></td>
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<table>
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<td id="rcorners1" align=center><a class="one" href="https://2015.igem.org/Team:METU_Turkey/Sponsors">SPONSORS</a></td>
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<h4 align=center>Gel Photos</h4>
 
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<tr>
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<h2 align=center>mCherry(restricted)-GFP-GFP</h2>
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<img hspace="375px" align=center src="https://static.igem.org/mediawiki/2015/thumb/9/97/MCherry%28restricted%29-GFP-GFP-PGap-z.JPG/319px-MCherry%28restricted%29-GFP-GFP-PGap-z.JPG" width="600px">
 +
</tr>
 +
<tr>
 +
<h2 align=center>101-106-117-mcherry-pgap-pgap-pgap-pgap-mcherry</h2>
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<img hspace="375px" align=center src="https://static.igem.org/mediawiki/2015/f/fa/101-106-117-mcherry-pgap-pgap-pgap-pgap-mcherryLAST.PNG" width="600px">
 +
</tr>
 +
<tr>
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<h2 align=center>KumamolisinMAX-strain3b-KumamolisinMAX-stain4a-2(compability)-mCherry(nice)-1(compability)-mCherry-mCherry</h2>
 +
<img hspace="375px" align=center src="https://static.igem.org/mediawiki/2015/0/06/Kumma123metu.PNG" width="600px">
  
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<img align=center src="https://static.igem.org/mediawiki/2015/f/f9/LO_ODTU_METU_ACIK_RENKLI_ZEMIN.png" width=100%></br>
 
  
  
 
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<img hspace="375px" align=center src="https://static.igem.org/mediawiki/2015/0/00/Celiac_stockphoto_1.jpg" width="600px">
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<img hspace="375px" align=center src="https://static.igem.org/mediawiki/2015/d/d6/Future_prospect131d.PNG" width="600px">
 
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<div class="highlightBox">
 
<div class="highlightBox">
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<h2 align=center>Project Description</h2>
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<p align=center>As an autoimmune disorder, celiac disorder depends on sensitivity to wheat ingredients such that gliadin which is a glycoprotein complex rich in proline and glutamine, especially alpha-2-gliadin. Alfa-2-glaidin basically bind to of specific receptor called HLA-DQ2 or HLA-DQ8 (because tTG alter the alfa-2-gliadin’s charge density which results in highly negative charges. This negative charge density create significant binding effect of alfa-2-gliadin to HLA-DQ2 OR HLA-DQ8 ). That receptor is an important key component of immune system, which have loci at chromosome 6. Epitopes of that receptor create cellular signaling pathway which results in arise of T and B-cells. These important immune cells eventually kill the specific antigens presented cells. Thus, in order to interfere this autoimmune disorder, protein’s (gluten) specific subunits, which have key roles at celiac disorder are going to be destroyed before the intake of protein in our project. Fundamentally, adding specific enzymes’ genes to E.coli’s genome. Then, E.coli will be added into dough while it is in fermentation process. So during the process of fermentation, specific enzymes’ genes will be transcribed and break down the gluten. To sum up, with the specific enzyme, which is expressed by E.coli, alters and break down the specific protein subunit which is key component of celiac disorder. In addition, we would like to improve the stiffness of gluten-free bread by letting bacteria to secrete additives. For the efficiency of the gluten destruction process, the homogenization of dough will be also improved by the bacteria secretion of additives. Last but not least, receptor complex within E.coli is going to be designed and enable bacteria to express receptor which is like HLA-DQ; in order to detect whether there is a gluten or not.</p>
+
<p align=center>This year we decided to work on e-coli so that we going to follow a guaranteed way to make our necessary experiments and observations. This helps us to gain an improved safety. When we consider about future of this project, we primarily aim to overcome problems with gluten-free products such as variety and price. Beside of these problems, we also aim to supply people a practical way which can help them to make their own bread at their homes. If doing gluten-free bread will be easy then other products also will be easily available. These home make kits will be time saving for celiac disease people. As shown in the gluten intolerance survey results, most of the surveyed people support us about this idea. In reality, working on yeast will be an important step to improve less cost and time saving way to produce gluten-free products. Working with p GAPZ A vector generally produce greater yields than inducible expression.
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</p>
 
</div>
 
</div>
 
</tr>
 
</tr>
 
</table>
 
</table>
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</div>
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</table>
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        <tr>
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        <td align=center>
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<a href="http://www.metu.edu.tr" target="_blank"> <img src="https://static.igem.org/mediawiki/2014/9/91/Metulogo.jpg" width="350px" align="center"></a>
 +
        </td>
 +
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    </table>
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<table  id="firstblock" width="60%"  cellspacing="10px" height="200px" align="center">
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 +
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<tr>
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 +
<td align="center" width="162px"><a href="http://odtuteknokent.com.tr/" target="_blank"> <img src="https://static.igem.org/mediawiki/2014/4/47/Odtuteknokent.jpg" width="175px"></a></td>
 +
 +
<td align="center" width="162px"><a href="http://www.kardiosis.com.tr/" target="_blank"> <img src="https://static.igem.org/mediawiki/2014/d/d4/Kardiosis.jpg" width="175px"></a></td>
 +
 +
<td align="center" width="162px"><a href="http://www.tr-idea.com/" target="_blank"> <img src="https://static.igem.org/mediawiki/2015/c/c6/METU_Turkey_Idea.PNG" width="350px"></a></td>
 +
 +
</tr>
 +
</table>
 +
<table align="center" cellspacing="10px">
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<tr>
 +
 +
<td align="center"><a href="http://www.sentegen.com/" target="_blank"> <img src="https://static.igem.org/mediawiki/2014/d/dd/Sentegenlogoder.PNG" width="175px"></a></td>
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 +
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 +
<td align="center"><a href="http://www.mathworks.com/" target="_blank"> <img src="https://static.igem.org/mediawiki/2015/a/ab/METU_Turkey_mathworks.png" width="175px"></a></td>
 +
 +
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Latest revision as of 13:29, 4 October 2015

Welcome!
We are Team METU_Turkey!

Gel Photos

mCherry(restricted)-GFP-GFP

101-106-117-mcherry-pgap-pgap-pgap-pgap-mcherry

KumamolisinMAX-strain3b-KumamolisinMAX-stain4a-2(compability)-mCherry(nice)-1(compability)-mCherry-mCherry

This year we decided to work on e-coli so that we going to follow a guaranteed way to make our necessary experiments and observations. This helps us to gain an improved safety. When we consider about future of this project, we primarily aim to overcome problems with gluten-free products such as variety and price. Beside of these problems, we also aim to supply people a practical way which can help them to make their own bread at their homes. If doing gluten-free bread will be easy then other products also will be easily available. These home make kits will be time saving for celiac disease people. As shown in the gluten intolerance survey results, most of the surveyed people support us about this idea. In reality, working on yeast will be an important step to improve less cost and time saving way to produce gluten-free products. Working with p GAPZ A vector generally produce greater yields than inducible expression.