Difference between revisions of "Team:Cambridge-JIC/Notebook"

graph.commit('march', '', $('<div style="margin-top:-40px"><h4>Marchantia</h4> Prepared some microscopic slides with fluorescent <i>Marchantia</i> transformants (express GFP), generously provided by the Haseloff Lab. However, able to look at them only through the lab fluorescence microscope for now. In the meantime, enjoy this simple <i>Marchantia</i> sample prep protocol: <ol> <li>Use No. 8 cork borer to cut cylinders of carrot core approx. 50 mm long.</li> <li>Place in 70% ethanol and leave overnight (will lose colour).</li> <li>Cut cylinders in half longitudinally, and place sample between halves (thin sample e.g. <i>Marchantia</i> leaf).</li> <li>Put halves back together with sample at one end, sandwiched in place.</li> <li>Insert the cylinder of carrot into a hand-held microtome.</li> <li>Cut thin slices of carrot + sample using microtome knife.</li> <li>Wash thin sections into beaker using deionised water.</li> <li>Use a pasteur pipette to pick up sample in a drop of water, and drop onto a slide (note: do not include carrot tissue).</li> <li>Cover with glass slip and visualise (brightfield or fluorescence).</li></ol></div>'));

graph.commit('march', '', $('<div style="margin-top:-40px"><h4>Marchantia</h4> Prepared some microscopic slides with fluorescent <i>Marchantia</i> transformants (express GFP), generously provided by the Haseloff Lab. However, able to look at them only through the lab fluorescence microscope for now. In the meantime, enjoy this simple <i>Marchantia</i> sample prep protocol: <ol> <li>Use No. 8 cork borer to cut cylinders of carrot core approx. 50 mm long.</li> <li>Place in 70% ethanol and leave overnight (will lose colour).</li> <li>Cut cylinders in half longitudinally, and place sample between halves (thin sample e.g. <i>Marchantia</i> leaf).</li> <li>Put halves back together with sample at one end, sandwiched in place.</li> <li>Insert the cylinder of carrot into a hand-held microtome.</li> <li>Cut thin slices of carrot + sample using microtome knife.</li> <li>Wash thin sections into beaker using deionised water.</li> <li>Use a pasteur pipette to pick up sample in a drop of water, and drop onto a slide (note: do not include carrot tissue).</li> <li>Cover with glass slip and visualise (brightfield or fluorescence).</li></ol></div>'));

graph.commit('optics', '28 July 2015', $('<div><h4>Optics</h4> Final tinkering with the optics for bright field microscopy. Done! We managed to achieve a full-colour image by simply changing our illumination to a bright white LED. Done with this now! Getting ready for fluorescence: printing a redesigned cube with our new 3D printer.</div>'));

graph.commit('optics', '28 July 2015', $('<div><h4>Optics</h4> Final tinkering with the optics for bright field microscopy. Done! We managed to achieve a full-colour image by simply changing our illumination to a bright white LED. Done with this now! Getting ready for fluorescence: printing a redesigned cube with our new 3D printer.</div>'));

graph.commit('sw', '', $('<div style="margin-top:-40px"><h4>Software</h4> We went crazy, pulled a late night yesterday and also spent this morning making our wiki design mostly fully responsive. Now we have one template to rule them all, and full mobile browser support!</div>'));

graph.commit('sw', '', $('<div style="margin-top:-40px"><h4>Software</h4> We went crazy, pulled a late night yesterday and also spent this morning making our wiki design mostly fully responsive. Now we have one template to rule them all, and full mobile browser support!</div>'));