Difference between revisions of "Team:Glasgow/Notebook"
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| + | <h1><strong>Vilija’s carrot cake</strong></h1><p>So good, honestly.</p> | ||
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| − | <p></p> | + | <h1><strong>Bioluminescence</strong></h1><p>Tested K325909 and K769020 in TOP10 to see how long until the bioluminescence started after addition of arabinose, how bright it is, and how LumP affects it.</p> |
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| + | <h1><strong>Repressors</strong></h1><p>Ligated both PPhlF and PSrpR with E5501 and I13500 in pSB1C3, respectively, and transformed into TOP10 and DH5α. C0040 (TetR), R0040 (PTetR) and backbone pSB3K3 (with insert J04450) off distribution plates – to use C0040 as a control for our repressors, and to use pSB3K3 as a second plasmid to have the promoters and repressors on separate plasmids. Ordered G-Block of R0011.B0032 (PLacI + RBS) for C0040 – as the plasmid on the plate has to RBS.</p> | ||
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| − | <p></p> | + | <h1><strong>UVA sensor</strong></h1><p>Found genomic DNA from Synechocystis PCC6803 to use for PCR. Ordered PLsiR as a G-Block with BioBrick prefix and suffix.</p> |
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| − | <div class="timeline-article"> | + | <div class="timeline-start" id="Week2">Week2</div> |
| + | <div class="timeline-article"> | ||
<div class="content-left-container"> | <div class="content-left-container"> | ||
<div class="content-left"> | <div class="content-left"> | ||
| + | <h1><strong>Vilija’s carrot cake</strong></h1><p>So good, honestly.</p> | ||
</div> | </div> | ||
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<div class="content-right-container"> | <div class="content-right-container"> | ||
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<div class="content-right-container"> | <div class="content-right-container"> | ||
<div class="content-right"> | <div class="content-right"> | ||
| − | <p></p> | + | <h1><strong>Bioluminescence</strong></h1><p>Tested K325909 and K769020 in TOP10 to see how long until the bioluminescence started after addition of arabinose, how bright it is, and how LumP affects it.</p> |
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| + | <h1><strong>Repressors</strong></h1><p>Ligated both PPhlF and PSrpR with E5501 and I13500 in pSB1C3, respectively, and transformed into TOP10 and DH5α. C0040 (TetR), R0040 (PTetR) and backbone pSB3K3 (with insert J04450) off distribution plates – to use C0040 as a control for our repressors, and to use pSB3K3 as a second plasmid to have the promoters and repressors on separate plasmids. Ordered G-Block of R0011.B0032 (PLacI + RBS) for C0040 – as the plasmid on the plate has to RBS.</p> | ||
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</div> | </div> | ||
<div class="content-right-container"> | <div class="content-right-container"> | ||
<div class="content-right"> | <div class="content-right"> | ||
| − | <p></p> | + | <h1><strong>UVA sensor</strong></h1><p>Found genomic DNA from Synechocystis PCC6803 to use for PCR. Ordered PLsiR as a G-Block with BioBrick prefix and suffix.</p> |
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| − | <div class="timeline-article"> | + | <div class="timeline-start" id="Week2">Week2</div> |
| + | <div class="timeline-article"> | ||
<div class="content-left-container"> | <div class="content-left-container"> | ||
<div class="content-left"> | <div class="content-left"> | ||
| + | <h1><strong>Vilija’s carrot cake</strong></h1><p>So good, honestly.</p> | ||
</div> | </div> | ||
</div> | </div> | ||
<div class="content-right-container"> | <div class="content-right-container"> | ||
<div class="content-right"> | <div class="content-right"> | ||
| − | < | + | <img src="https://static.igem.org/mediawiki/2015/6/6b/2015-Glasgow-timeline6.jpg" style="max-width:350px;"> |
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<div class="content-right-container"> | <div class="content-right-container"> | ||
<div class="content-right"> | <div class="content-right"> | ||
| − | <p></p> | + | <h1><strong>Bioluminescence</strong></h1><p>Tested K325909 and K769020 in TOP10 to see how long until the bioluminescence started after addition of arabinose, how bright it is, and how LumP affects it.</p> |
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| + | <h1><strong>Repressors</strong></h1><p>Ligated both PPhlF and PSrpR with E5501 and I13500 in pSB1C3, respectively, and transformed into TOP10 and DH5α. C0040 (TetR), R0040 (PTetR) and backbone pSB3K3 (with insert J04450) off distribution plates – to use C0040 as a control for our repressors, and to use pSB3K3 as a second plasmid to have the promoters and repressors on separate plasmids. Ordered G-Block of R0011.B0032 (PLacI + RBS) for C0040 – as the plasmid on the plate has to RBS.</p> | ||
</div> | </div> | ||
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<div class="content-right-container"> | <div class="content-right-container"> | ||
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| − | <div class="timeline-article"> | + | |
<div class="content-left-container"> | <div class="content-left-container"> | ||
<div class="content-left"> | <div class="content-left"> | ||
| + | <p></p> | ||
</div> | </div> | ||
</div> | </div> | ||
<div class="content-right-container"> | <div class="content-right-container"> | ||
<div class="content-right"> | <div class="content-right"> | ||
| − | <p></p> | + | <h1><strong>UVA sensor</strong></h1><p>Found genomic DNA from Synechocystis PCC6803 to use for PCR. Ordered PLsiR as a G-Block with BioBrick prefix and suffix.</p> |
</div> | </div> | ||
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Revision as of 14:57, 13 August 2015
James’ muffins
Talked to Professors Marshall Stark and John Christie about our project.
Repressors
Ordered PPhlF and PSrpR with BioBrick prefix and suffix as oligos. Ordered PhlF and SrpR as G-blocks, with ribosome binding site B0032, terminator B0010+ (a longer version of B0010, that we will characterise and submit as a new BioBrick) and BioBrick prefix and suffix. Got E5501 and I13500 – GFP (E0040) with ribosome binding sites B0032 and B0034 respectively – in pSB1C3 in DH5α from glycerol stocks from last year’s iGEM team.
UVA sensor
Designed primers to PCR UirS and UirR from Synechocystis PCC6803 genomic DNA with ribosome binding site B0032 and BioBrick prefix and suffix. Tested UVB sensitivity of strains TOP10 and DH5α (recA- - very poor DNA repair), DS941 (recF- - limited DNA repair), and MG1655.Z1 (wild type for DNA repair).
Bioluminescence
pBAD.LuxCDABEG (K325909) and pBAD.LuxCDABEG.LumP (K769020) off distribution plates and transformed into TOP10 and DH5α. Designed primers to PCR LuxA, B, C, D, E, and G individually with ribosome binding site B0032 and BioBrick prefix and suffix from K325909.
Vilija’s carrot cake
So good, honestly.
Bioluminescence
Tested K325909 and K769020 in TOP10 to see how long until the bioluminescence started after addition of arabinose, how bright it is, and how LumP affects it.
Repressors
Ligated both PPhlF and PSrpR with E5501 and I13500 in pSB1C3, respectively, and transformed into TOP10 and DH5α. C0040 (TetR), R0040 (PTetR) and backbone pSB3K3 (with insert J04450) off distribution plates – to use C0040 as a control for our repressors, and to use pSB3K3 as a second plasmid to have the promoters and repressors on separate plasmids. Ordered G-Block of R0011.B0032 (PLacI + RBS) for C0040 – as the plasmid on the plate has to RBS.
UVA sensor
Found genomic DNA from Synechocystis PCC6803 to use for PCR. Ordered PLsiR as a G-Block with BioBrick prefix and suffix.
Vilija’s carrot cake
So good, honestly.
Bioluminescence
Tested K325909 and K769020 in TOP10 to see how long until the bioluminescence started after addition of arabinose, how bright it is, and how LumP affects it.
Repressors
Ligated both PPhlF and PSrpR with E5501 and I13500 in pSB1C3, respectively, and transformed into TOP10 and DH5α. C0040 (TetR), R0040 (PTetR) and backbone pSB3K3 (with insert J04450) off distribution plates – to use C0040 as a control for our repressors, and to use pSB3K3 as a second plasmid to have the promoters and repressors on separate plasmids. Ordered G-Block of R0011.B0032 (PLacI + RBS) for C0040 – as the plasmid on the plate has to RBS.
UVA sensor
Found genomic DNA from Synechocystis PCC6803 to use for PCR. Ordered PLsiR as a G-Block with BioBrick prefix and suffix.
Vilija’s carrot cake
So good, honestly.
Bioluminescence
Tested K325909 and K769020 in TOP10 to see how long until the bioluminescence started after addition of arabinose, how bright it is, and how LumP affects it.
Repressors
Ligated both PPhlF and PSrpR with E5501 and I13500 in pSB1C3, respectively, and transformed into TOP10 and DH5α. C0040 (TetR), R0040 (PTetR) and backbone pSB3K3 (with insert J04450) off distribution plates – to use C0040 as a control for our repressors, and to use pSB3K3 as a second plasmid to have the promoters and repressors on separate plasmids. Ordered G-Block of R0011.B0032 (PLacI + RBS) for C0040 – as the plasmid on the plate has to RBS.
UVA sensor
Found genomic DNA from Synechocystis PCC6803 to use for PCR. Ordered PLsiR as a G-Block with BioBrick prefix and suffix.
Vilija’s carrot cake
So good, honestly.
Bioluminescence
Tested K325909 and K769020 in TOP10 to see how long until the bioluminescence started after addition of arabinose, how bright it is, and how LumP affects it.
Repressors
Ligated both PPhlF and PSrpR with E5501 and I13500 in pSB1C3, respectively, and transformed into TOP10 and DH5α. C0040 (TetR), R0040 (PTetR) and backbone pSB3K3 (with insert J04450) off distribution plates – to use C0040 as a control for our repressors, and to use pSB3K3 as a second plasmid to have the promoters and repressors on separate plasmids. Ordered G-Block of R0011.B0032 (PLacI + RBS) for C0040 – as the plasmid on the plate has to RBS.
UVA sensor
Found genomic DNA from Synechocystis PCC6803 to use for PCR. Ordered PLsiR as a G-Block with BioBrick prefix and suffix.