Difference between revisions of "Team:UMaryland/Description"

Line 14: Line 14:
  
 
<style type="text/css">
 
<style type="text/css">
−
 
 
#squeeze {
 
#squeeze {
 
margin:auto;
 
margin:auto;
Line 23: Line 22:
 
}
 
}
 
#squeeze h1,h2,h3,h4,p,img,a {
 
#squeeze h1,h2,h3,h4,p,img,a {
−
  width: 80%;
+
width: 80%;
−
  margin: auto;
+
margin: auto;
−
  }
+
}
 +
#cover {
 +
overflow:hidden;
 +
position:relative;
 +
width:100%;
 +
margin:auto;
 +
padding:0px;
 +
background-image: url("http://www.swedenrecycling.se/wp-content/uploads/Provtagning_colourbox.jpg");
 +
background-repeat: no-repeat;
 +
height: 100%;
 +
width:100%;
 +
min-height:1109;
 +
min-width:675;
 +
color: red;
 +
font-family: Tahoma, Geneva, sans-serif;
 +
font-size:xx-large;
 +
text-align: center;
 +
bottom:-35px;
 +
}
 +
h1 {
 +
font-size: 250%;
 +
}
 +
h2 {
 +
font-size: 200%;
 +
}
 +
h3 {
 +
font-size: 150%;
 +
}
 +
h4 {
 +
font-size: 125%;
 +
}
 +
p {
 +
font-size: 100%;
 +
}
 +
 
 
#layer1 {
 
#layer1 {
−
top:10%;
 
 
overflow:hidden;
 
overflow:hidden;
 
position:relative;
 
position:relative;
Line 33: Line 65:
 
margin:auto;
 
margin:auto;
 
padding:0px;
 
padding:0px;
−
background-color:#F4FA58;
+
background-image: -webkit-gradient(linear, left bottom, right top, color-stop(0, #74CF83), color-stop(50, #FFFFFF), color-stop(100, #74CF83));
 
min-height:42px;
 
min-height:42px;
 
}
 
}
Line 42: Line 74:
 
margin:auto;
 
margin:auto;
 
padding:0px;
 
padding:0px;
−
background-color:#58FA58;
+
background-image: -webkit-gradient(linear, left bottom, right top, color-stop(0, #5CF2F2), color-stop(50, #FFFFFF), color-stop(100, #5CF2F2));
 
min-height:42px;
 
min-height:42px;
 
}
 
}
Line 54: Line 86:
 
min-height:42px;
 
min-height:42px;
 
}
 
}
−
#hoksokL {
+
#button {
−
float:left;
+
overflow:hidden;
−
}
+
display: inline-block;
−
#hoksokR {
+
height:120px;
−
float:right;
+
width: 240px;
 +
background-image: -webkit-gradient(linear, left bottom, left top, color-stop(0, #ffffff), color-stop(100, #FF0000));
 +
border: 2px solid black;
 +
border-radius: 10px;
 +
margin: 25px;
 +
padding:10px;
 +
font-size:medium;
 +
color:black;
 +
text-align: center;
 
}
 
}
 
</style>
 
</style>
  
 +
<div id='cover'>
 +
 +
<br>
 +
<br>
 +
<h1>Safe and Inexpensive</h1>
 +
 +
<br>
 +
<h2><u>Approaches to Advance Synthetic Biology</u></h2>
 +
<br>
 +
<br>
 +
 +
<a href="#HS">
 +
<div id='button'>
 +
<h4>Hok/Sok</h4>
 +
<p>A Pladmid retention system
 +
</div>
 +
</a>
 +
<a href="#PCR">
 +
<div id='button'>
 +
<h4>CHIP</h4>
 +
<p>Our homemade PCR
 +
</div>
 +
</a>
 +
<a href="#">
 +
<div id='button'>
 +
<h4>IL Study</h4>
 +
<p>A study across iGEM labs
 +
</div>
 +
</a>
 +
<a href="#">
 +
<div id='button'>
 +
<h4>Side projects</h4>
 +
<p>Other projects we've worked on
 +
</div>
 +
</a>
 +
<br>
 +
<br>
 +
</div>
  
 
<div id='layer1'>
 
<div id='layer1'>
−
<img src="http://carleton.ca/chemistry/wp-content/uploads/prog-banner-masters-chemistry.jpg"style="height:100%; width:100%;">
 
 
<div id='squeeze'>
 
<div id='squeeze'>
−
<h1>
 
−
Safe and Inexpensive Approaches to Advance Synthetic Biology
 
−
</h1>
 
−
<h3>TL;DR: We have two projects! One is antibiotic free plasmid retention, the other is a cheap PCR machine!</h3>
 
 
<br>
 
<br>
 
<p>Abstract: Alternative methods of plasmid maintenance and PCR amplification accelerate the construction of new biodesigns, reduce cost, and avoid environmental hazards. Plasmids are typically maintained in cells by encoding enzymes that hydrolyze or otherwise detoxify antibiotics added to the medium. However, this process carries an inherent risk for spreading antibiotic resistance to native bacterial populations through lateral gene transfer. The Hok-Sok toxin-antitoxin system, a natural internal maintenance cassette relying on internal mRNA silencing, presents an alternative to common antibiotic-based methods since it does not rely on exogenous drugs. We are also developing an integrated, microcontrolled thermocycler using common household components. Using nichrome wire and a motorized fan for air circulation, the programmable prototype is an inexpensive, versatile thermocycler or plate incubator. Because the material and construction costs are a fraction of dedicated instruments, the newly developed unit will find broad application among nascent synthetic biologists in underfunded environments.
 
<p>Abstract: Alternative methods of plasmid maintenance and PCR amplification accelerate the construction of new biodesigns, reduce cost, and avoid environmental hazards. Plasmids are typically maintained in cells by encoding enzymes that hydrolyze or otherwise detoxify antibiotics added to the medium. However, this process carries an inherent risk for spreading antibiotic resistance to native bacterial populations through lateral gene transfer. The Hok-Sok toxin-antitoxin system, a natural internal maintenance cassette relying on internal mRNA silencing, presents an alternative to common antibiotic-based methods since it does not rely on exogenous drugs. We are also developing an integrated, microcontrolled thermocycler using common household components. Using nichrome wire and a motorized fan for air circulation, the programmable prototype is an inexpensive, versatile thermocycler or plate incubator. Because the material and construction costs are a fraction of dedicated instruments, the newly developed unit will find broad application among nascent synthetic biologists in underfunded environments.
Line 79: Line 152:
 
<div id='layer2'>
 
<div id='layer2'>
 
<div id='squeeze'>
 
<div id='squeeze'>
−
<h1> Hok/Sok </h1>
+
<a name="HS"><h1> Hok/Sok </h1></a>  
 
</a>
 
</a>
  
 
<p>
 
<p>
−
 
 
Antibiotic resistance is a necessary selection factor for transgenic bacteria using plasmids as vectors. This staple of genetic engineering has been met with opposition with valid claims that the addition of antibiotics to the environment harms native species and poses a risk to unwanted antibiotic resistance through lateral gene transfer.
 
Antibiotic resistance is a necessary selection factor for transgenic bacteria using plasmids as vectors. This staple of genetic engineering has been met with opposition with valid claims that the addition of antibiotics to the environment harms native species and poses a risk to unwanted antibiotic resistance through lateral gene transfer.
 
<p>
 
<p>
Line 90: Line 162:
  
 
<div  display=inline-block;>
 
<div  display=inline-block;>
−
 
−
 
  
 
<br>
 
<br>
Line 111: Line 181:
 
</div>
 
</div>
  
−
<div id='layer3'>
+
<div id='layer1'>
 
<div id='squeeze'>
 
<div id='squeeze'>
 
<br>
 
<br>
 +
<a name="PCR"><h1>Thermocycler</h1></a>
  
−
 
+
<br>
−
 
+
−
 
+
−
<h1>Thermocycler </h1>
+
−
</a>
+
−
  <br>
+
 
<br>
 
<br>
  

Revision as of 02:23, 7 September 2015