Difference between revisions of "Team:ETH Zurich/Materials"

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{{ETH_Zurich}}
 
{{ETH_Zurich}}
 
<html>
 
<html>
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<div class="expContainer">
 
<div class="expContainer">
 
<h1>Materials</h1>
 
<h1>Materials</h1>
  
 +
<h2>Bacterial strains and Mammalian Cell Lines</h2>
 +
 +
<h3>TOP10</h3>
 +
<p>The bacterial strain we used as a base for all our transformations was TOP10.</p>
 +
 +
<p>A list of all strains we generated in the course of our project can be found here (link).</p>
 +
 +
<h3>Jurkat Cells</h3>
 +
 +
<p>Immortalized human T lymphocyte cell line. We used this cell line as a model line for cancer cells with elevated lactate production and TRAIL susceptibility (papers).</p>
 +
 +
<h3>HL60</h3>
 +
 +
<p>Cell line derived from acute myeloid leukemia. We used this cell line as a model line for cancer cells with elevated lactate production and TRAIL susceptibility (papers).</p>
 +
 +
 +
<h3>3T3</h3>
 +
 +
<p>Murine fibroblast cell line. We used this cell line as a model line for healthy cells which are restistant to TRAIL and produce normal levels of lactate.</p>
 +
 +
<h3>SK-BR-3</h3>
 +
 +
<p>HER2-positive breast cancer cell line. We used this cell line to validate the chimeric receptors of the <a href="https://2015.igem.org/Team:Stockholm">Stockholm</a> iGEM team.</p>
 +
 +
</div>
 +
 +
 +
 +
<div class="expContainer">
 +
 +
<h2>Machines</h2>
 +
 +
 +
 +
<div class="info">
 +
 +
<h3>Nano Drop</h3>
 +
<ul>
 +
<li>Thermo Scientific NanoDrop® 2000 UV-Vis spectrophotometer combined with NanoDrop 2000 / 2000c Software</li>
 +
<a href="http://www.nanodrop.com/Productnd2000overview.aspx"><p>for further information click here</p></a>
 +
</html>
 +
[[File:NanoDrop.JPG|center|thumb]]
 +
<html>
 +
</ul>
 +
<a class="expander" href="#" onclick="expand(this);return false;"><img src="https://static.igem.org/mediawiki/2015/1/1f/Blank_square.png"></a>
 +
</div>
 +
 +
 +
 +
 +
<div class="info">
 +
 +
<h3>Plate Reader</h3>
 +
<ul>
 +
<li>Tecan Infinite M200 Pro™ combined with Tecan iControl TM software</li>
 +
<a href="http://www.tecan.com/platform/apps/product/index.asp?MenuID=1812&ID=1916&Menu=1&Item=21.2.10.1"><p>for further information (platereader) click here</p></a>
 +
<a href="http://www.tecan.ch/platform/apps/product/index.asp?MenuID=1817&ID=1924&Menu=1&Item=21.7.8"><p>for further information (software) click here</p></a>
 +
</html>
 +
[[File:TecanPlateReader.JPG|center|thumb]]
 +
<html>
 +
</html>
 +
[[File:ProgramTecanReader.JPG|center|thumb]]
 +
<html>
 +
</ul>
 +
<a class="expander" href="#" onclick="expand(this);return false;"><img src="https://static.igem.org/mediawiki/2015/1/1f/Blank_square.png"></a>
 +
</div>
 +
 +
 +
 +
<div class="info">
 +
 +
<h3>Absorbance Measurments</h3>
 +
<ul>
 +
<li>Novaspec Plus™ Visible Spectrophotometer</li>
 +
<a href="http://www.gelifesciences.com/webapp/wcs/stores/servlet/ProductDisplay?categoryId=11012&catalogId=10101&productId=20430&storeId=11782&langId=-1"><p>for further information click here</p></a>
 +
</html>
 +
[[File:ODPhotometer.JPG|center|thumb]]
 +
<html>
 +
</ul>
 +
<a class="expander" href="#" onclick="expand(this);return false;"><img src="https://static.igem.org/mediawiki/2015/1/1f/Blank_square.png"></a>
 +
</div>
 +
 +
 +
 +
<div class="info">
 +
 +
<h3>UV- and Dark Hood</h3>
 +
<ul>
 +
<li>Biostep Dark-Hood DH-50™ combined with Argus-X1™ software and Biostep argusX1™ basic licence</li>
 +
<a href="http://www.biostep.de/i18n_en_DE/products/Bio_Imaging_components_1776/Tripod__dark_hoods_and_accessories_1863/Dark_hoods_1865/Dark_Hood_DH_40_with_control_panel_and_display_6/index.html"><p>for further information (hood) click here</p></a>
 +
<a href="https://www.biostep.de/products/Bio_Imaging_software_1843/Acquisition_and_control_software_1844/argus_X1___for_cameras_and_scanners_1845/biostep_argusX1__basic_licence_1/index.html"><p>for further information (software) click here</p></a>
 +
</html>
 +
[[File:Darkhood.JPG|center|thumb]]
 +
<html>
 +
</ul>
 +
<a class="expander" href="#" onclick="expand(this);return false;"><img src="https://static.igem.org/mediawiki/2015/1/1f/Blank_square.png"></a>
 +
</div>
 +
 +
 +
 +
<div class="info">
 +
 +
<h3>Large Centrifuge</h3>
 +
<ul>
 +
<li>Eppendorf Centrifuge 5810 R</li>
 +
<a href="http://www.eppendorf.com/int/index.php?pb=01edea0fe7a0e358&action=products&contentid=1&catalognode=10019"><p>for further information click here</p></a>
 +
</html>
 +
[[File:BigCentrifuge.JPG|center|thumb]]
 +
<html>
 +
</ul>
 +
<a class="expander" href="#" onclick="expand(this);return false;"><img src="https://static.igem.org/mediawiki/2015/1/1f/Blank_square.png"></a>
 +
</div>
 +
 +
 +
 +
<div class="info">
 +
 +
<h3>Small Centrifuge</h3>
 +
<ul>
 +
<li>Eppendorf Centrifuge 5424 R</li>
 +
<a href="http://www.labdepotinc.com/Product_Details~id~450~pid~59833.aspx"><p>for further information click here</p></a>
 +
</html>
 +
[[File:EppiCentrifuge.JPG|center|thumb]]
 +
<html>
 +
</ul>
 +
<a class="expander" href="#" onclick="expand(this);return false;"><img src="https://static.igem.org/mediawiki/2015/1/1f/Blank_square.png"></a>
 +
</div>
 +
 +
 +
 +
<div class="info">
 +
 +
<h3>Shaker</h3>
 +
<ul>
 +
<li>Kuhner Climo-Shaker ISF1-X</li>
 +
<a href="http://www.kuhner.com/de/produkte/schuettelmaschinen/inkubatoren/isf1-x.html"><p>for further information click here</p></a>
 +
</html>
 +
[[File:ShakerIncubator.JPG|center|thumb]]
 +
<html>
 +
</ul>
 +
<a class="expander" href="#" onclick="expand(this);return false;"><img src="https://static.igem.org/mediawiki/2015/1/1f/Blank_square.png"></a>
 +
</div>
 +
 +
 +
 +
<div class="info">
 +
 +
<h3>PCR Machine</h3>
 +
<ul>
 +
<li>Applied Biosystems Veriti 96Well Thermal Cycler</li>
 +
<a href="https://www.thermofisher.com/ch/en/home/life-science/pcr/thermal-cyclers-realtime-instruments/thermal-cyclers/veriti-thermal-cycler.html"><p>for further information click here</p></a>
 +
</html>
 +
[[File:PCRMachine.JPG|center|thumb]]
 +
<html>
 +
</ul>
 +
<a class="expander" href="#" onclick="expand(this);return false;"><img src="https://static.igem.org/mediawiki/2015/1/1f/Blank_square.png"></a>
 +
</div>
 +
 +
 +
 +
<div class="info">
 +
 +
<h3>Gel Electrophoresis</h3>
 +
<ul>
 +
<li>Helixx Technologies MyRun®</li>
 +
<a href="http://www.helixxtec.com/helixx/c/iMyRun/iMyRun.htm"><p>for further information click here</p></a>
 +
</html>
 +
[[File:GelRunningThing.JPG|center|thumb]]
 +
<html>
 +
</ul>
 +
<a class="expander" href="#" onclick="expand(this);return false;"><img src="https://static.igem.org/mediawiki/2015/1/1f/Blank_square.png"></a>
 +
</div>
 +
 +
 +
 +
<div class="info">
 +
 +
<h3>Eppi-shaker/Heatingblock</h3>
 +
<ul>
 +
<li>Eppendorf Thermomixer compact</li>
 +
<a href="http://www.eppendorf.com/int/index.php?pb=93a83e2a7b7b1b05&action=products&contentid=1&catalognode=9840"><p>for further information click here</p></a>
 +
</html>
 +
[[File:HeatingBlock.JPG|center|thumb]]
 +
<html>
 +
</ul>
 +
<a class="expander" href="#" onclick="expand(this);return false;"><img src="https://static.igem.org/mediawiki/2015/1/1f/Blank_square.png"></a>
 +
</div>
 +
 +
 +
 +
<div class="info">
 +
 +
<h3>Vortex</h3>
 +
<ul>
 +
<li>Scientific Industries VortexGenie®2</li>
 +
<a href="http://www.scientificindustries.com/genie2.html"><p>for further information click here</p></a>
 +
</html>
 +
[[File:Vortex.JPG|center|thumb]]
 +
<html>
 +
</ul>
 +
<a class="expander" href="#" onclick="expand(this);return false;"><img src="https://static.igem.org/mediawiki/2015/1/1f/Blank_square.png"></a>
 +
</div>
 +
 +
 +
 +
<div class="info">
 +
 +
<h3>Balance</h3>
 +
<ul>
 +
<li>MettlerToledo XS4002S DeltaRange® Balance</li>
 +
<a href="http://us.mt.com/us/en/home/products/Laboratory_Weighing_Solutions/Precision_Balances/XS_Precision_Balances/XS_4002_S_Dual_Range.html"><p>for further information click here</p></a>
 +
</html>
 +
[[File:Balance.JPG|center|thumb]]
 +
<html>
 +
</ul>
 +
<a class="expander" href="#" onclick="expand(this);return false;"><img src="https://static.igem.org/mediawiki/2015/1/1f/Blank_square.png"></a>
 +
</div>
 +
 +
 +
 +
 +
<div class="info">
 +
 +
<h3>Incubator</h3>
 +
<ul>
 +
<li>Thermo Scientific Heraeus Incubator</li>
 +
<a href="https://webshop.fishersci.com/insight2_uk/getProduct.do?productCode=11626250&resultSetPosition=0"><p>for further information click here</p></a>
 +
</html>
 +
[[File:Incubator.JPG|center|thumb]]
 +
<html>
 +
</ul>
 +
<a class="expander" href="#" onclick="expand(this);return false;"><img src="https://static.igem.org/mediawiki/2015/1/1f/Blank_square.png"></a>
 +
</div>
 +
 +
 +
 +
 +
<div class="info">
 +
 +
<h3>FACS</h3>
 +
<ul>
 +
<li>BD LSRFortessa™ cell analyzer</li>
 +
<a href="http://www.bdbiosciences.com/us/instruments/research/cell-analyzers/bd-lsrfortessa/m/751752?cc=US"><p>for further information click here</p></a>
 +
</html>
 +
[[File:FACS.JPG|center|thumb]]
 +
<html>
 +
</ul>
 +
<a class="expander" href="#" onclick="expand(this);return false;"><img src="https://static.igem.org/mediawiki/2015/1/1f/Blank_square.png"></a>
 +
</div>
 +
 +
 +
 +
<div class="info">
 +
 +
<h3>Fluorescence Scanner for Western Blots</h3>
 +
<ul>
 +
<li>ODYSSEY® CLx Infrared Imaging System</li>
 +
<a href="http://www.licor.com/bio/products/imaging_systems/odyssey/"><p>for further information click here</p></a>
 +
</html>
 +
[[File:FluorescenceScanner.JPG|center|thumb]]
 +
<html>
 +
</ul>
 +
<a class="expander" href="#" onclick="expand(this);return false;"><img src="https://static.igem.org/mediawiki/2015/1/1f/Blank_square.png"></a>
 +
</div>
 +
 +
 +
<div class="info">
 +
 +
<h3>Emulsifier</h3>
 +
<ul>
 +
<li>EmulsiFlex-C3 from AVESTIN, Inc. and AVESTIN Europe GmbH/li>
 +
<a href="http://www.avestin.com/gc3page.html"><p>for further information click here</p></a>
 +
</ul>
 +
<a class="expander" href="#" onclick="expand(this);return false;"><img src="https://static.igem.org/mediawiki/2015/1/1f/Blank_square.png"></a>
 +
</div>
 +
 +
</div>
 +
 +
 +
 +
<div class="expContainer">
  
 
<h2>Buffers and media </h2>
 
<h2>Buffers and media </h2>
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</div>
 
</div>
  
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<div class="expContainer">
 
−
 
−
 
−
<h2>Bacterial strains and Mammalian Cell Lines</h2>
 
−
 
−
<h3>TOP10</h3>
 
−
<p>The bacterial strain we used as a base for all our transformations was TOP10.</p>
 
−
 
−
<p>A list of all strains we generated in the course of our project can be found here (link).</p>
 
−
 
−
<h3>Jurkat Cells</h3>
 
−
 
−
<p>Immortalized human T lymphocyte cell line. We used this cell line as a model line for cancer cells with elevated lactate production and TRAIL susceptibility (papers).</p>
 
−
 
−
<h3>HL60</h3>
 
−
 
−
<p>Cell line derived from acute myeloid leukemia. We used this cell line as a model line for cancer cells with elevated lactate production and TRAIL susceptibility (papers).</p>
 
−
 
−
 
−
<h3>3T3</h3>
 
−
 
−
<p>Murine fibroblast cell line. We used this cell line as a model line for healthy cells which are restistant to TRAIL and produce normal levels of lactate.</p>
 
−
 
−
<h3>SK-BR-3</h3>
 
−
 
−
<p>HER2-positive breast cancer cell line. We used this cell line to validate the chimeric receptors of the <a href="https://2015.igem.org/Team:Stockholm">Stockholm</a> iGEM team.</p>
 
−
 
−
</div>
 
−
 
−
 
−
 
−
<div class="expContainer">
 
−
 
−
<h2>Machines</h2>
 
−
 
−
 
−
 
−
<div class="info">
 
−
 
−
<h3>Nano Drop</h3>
 
−
<ul>
 
−
<li>Thermo Scientific NanoDrop® 2000 UV-Vis spectrophotometer combined with NanoDrop 2000 / 2000c Software</li>
 
−
<a href="http://www.nanodrop.com/Productnd2000overview.aspx"><p>for further information click here</p></a>
 
−
</html>
 
−
[[File:NanoDrop.JPG|center|thumb]]
 
−
<html>
 
−
</ul>
 
−
<a class="expander" href="#" onclick="expand(this);return false;"><img src="https://static.igem.org/mediawiki/2015/1/1f/Blank_square.png"></a>
 
−
</div>
 
−
 
−
 
−
 
−
 
−
<div class="info">
 
−
 
−
<h3>Plate Reader</h3>
 
−
<ul>
 
−
<li>Tecan Infinite M200 Pro™ combined with Tecan iControl TM software</li>
 
−
<a href="http://www.tecan.com/platform/apps/product/index.asp?MenuID=1812&ID=1916&Menu=1&Item=21.2.10.1"><p>for further information (platereader) click here</p></a>
 
−
<a href="http://www.tecan.ch/platform/apps/product/index.asp?MenuID=1817&ID=1924&Menu=1&Item=21.7.8"><p>for further information (software) click here</p></a>
 
−
</html>
 
−
[[File:TecanPlateReader.JPG|center|thumb]]
 
−
<html>
 
−
</html>
 
−
[[File:ProgramTecanReader.JPG|center|thumb]]
 
−
<html>
 
−
</ul>
 
−
<a class="expander" href="#" onclick="expand(this);return false;"><img src="https://static.igem.org/mediawiki/2015/1/1f/Blank_square.png"></a>
 
−
</div>
 
−
 
−
 
−
 
−
<div class="info">
 
−
 
−
<h3>Absorbance Measurments</h3>
 
−
<ul>
 
−
<li>Novaspec Plus™ Visible Spectrophotometer</li>
 
−
<a href="http://www.gelifesciences.com/webapp/wcs/stores/servlet/ProductDisplay?categoryId=11012&catalogId=10101&productId=20430&storeId=11782&langId=-1"><p>for further information click here</p></a>
 
−
</html>
 
−
[[File:ODPhotometer.JPG|center|thumb]]
 
−
<html>
 
−
</ul>
 
−
<a class="expander" href="#" onclick="expand(this);return false;"><img src="https://static.igem.org/mediawiki/2015/1/1f/Blank_square.png"></a>
 
−
</div>
 
−
 
−
 
−
 
−
<div class="info">
 
−
 
−
<h3>UV- and Dark Hood</h3>
 
−
<ul>
 
−
<li>Biostep Dark-Hood DH-50™ combined with Argus-X1™ software and Biostep argusX1™ basic licence</li>
 
−
<a href="http://www.biostep.de/i18n_en_DE/products/Bio_Imaging_components_1776/Tripod__dark_hoods_and_accessories_1863/Dark_hoods_1865/Dark_Hood_DH_40_with_control_panel_and_display_6/index.html"><p>for further information (hood) click here</p></a>
 
−
<a href="https://www.biostep.de/products/Bio_Imaging_software_1843/Acquisition_and_control_software_1844/argus_X1___for_cameras_and_scanners_1845/biostep_argusX1__basic_licence_1/index.html"><p>for further information (software) click here</p></a>
 
−
</html>
 
−
[[File:Darkhood.JPG|center|thumb]]
 
−
<html>
 
−
</ul>
 
−
<a class="expander" href="#" onclick="expand(this);return false;"><img src="https://static.igem.org/mediawiki/2015/1/1f/Blank_square.png"></a>
 
−
</div>
 
−
 
−
 
−
 
−
<div class="info">
 
−
 
−
<h3>Large Centrifuge</h3>
 
−
<ul>
 
−
<li>Eppendorf Centrifuge 5810 R</li>
 
−
<a href="http://www.eppendorf.com/int/index.php?pb=01edea0fe7a0e358&action=products&contentid=1&catalognode=10019"><p>for further information click here</p></a>
 
−
</html>
 
−
[[File:BigCentrifuge.JPG|center|thumb]]
 
−
<html>
 
−
</ul>
 
−
<a class="expander" href="#" onclick="expand(this);return false;"><img src="https://static.igem.org/mediawiki/2015/1/1f/Blank_square.png"></a>
 
−
</div>
 
−
 
−
 
−
 
−
<div class="info">
 
−
 
−
<h3>Small Centrifuge</h3>
 
−
<ul>
 
−
<li>Eppendorf Centrifuge 5424 R</li>
 
−
<a href="http://www.labdepotinc.com/Product_Details~id~450~pid~59833.aspx"><p>for further information click here</p></a>
 
−
</html>
 
−
[[File:EppiCentrifuge.JPG|center|thumb]]
 
−
<html>
 
−
</ul>
 
−
<a class="expander" href="#" onclick="expand(this);return false;"><img src="https://static.igem.org/mediawiki/2015/1/1f/Blank_square.png"></a>
 
−
</div>
 
−
 
−
 
−
 
−
<div class="info">
 
−
 
−
<h3>Shaker</h3>
 
−
<ul>
 
−
<li>Kuhner Climo-Shaker ISF1-X</li>
 
−
<a href="http://www.kuhner.com/de/produkte/schuettelmaschinen/inkubatoren/isf1-x.html"><p>for further information click here</p></a>
 
−
</html>
 
−
[[File:ShakerIncubator.JPG|center|thumb]]
 
−
<html>
 
−
</ul>
 
−
<a class="expander" href="#" onclick="expand(this);return false;"><img src="https://static.igem.org/mediawiki/2015/1/1f/Blank_square.png"></a>
 
−
</div>
 
−
 
−
 
−
 
−
<div class="info">
 
−
 
−
<h3>PCR Machine</h3>
 
−
<ul>
 
−
<li>Applied Biosystems Veriti 96Well Thermal Cycler</li>
 
−
<a href="https://www.thermofisher.com/ch/en/home/life-science/pcr/thermal-cyclers-realtime-instruments/thermal-cyclers/veriti-thermal-cycler.html"><p>for further information click here</p></a>
 
−
</html>
 
−
[[File:PCRMachine.JPG|center|thumb]]
 
−
<html>
 
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</ul>
 
−
<a class="expander" href="#" onclick="expand(this);return false;"><img src="https://static.igem.org/mediawiki/2015/1/1f/Blank_square.png"></a>
 
−
</div>
 
−
 
−
 
−
 
−
<div class="info">
 
−
 
−
<h3>Gel Electrophoresis</h3>
 
−
<ul>
 
−
<li>Helixx Technologies MyRun®</li>
 
−
<a href="http://www.helixxtec.com/helixx/c/iMyRun/iMyRun.htm"><p>for further information click here</p></a>
 
−
</html>
 
−
[[File:GelRunningThing.JPG|center|thumb]]
 
−
<html>
 
−
</ul>
 
−
<a class="expander" href="#" onclick="expand(this);return false;"><img src="https://static.igem.org/mediawiki/2015/1/1f/Blank_square.png"></a>
 
−
</div>
 
−
 
−
 
−
 
−
<div class="info">
 
−
 
−
<h3>Eppi-shaker/Heatingblock</h3>
 
−
<ul>
 
−
<li>Eppendorf Thermomixer compact</li>
 
−
<a href="http://www.eppendorf.com/int/index.php?pb=93a83e2a7b7b1b05&action=products&contentid=1&catalognode=9840"><p>for further information click here</p></a>
 
−
</html>
 
−
[[File:HeatingBlock.JPG|center|thumb]]
 
−
<html>
 
−
</ul>
 
−
<a class="expander" href="#" onclick="expand(this);return false;"><img src="https://static.igem.org/mediawiki/2015/1/1f/Blank_square.png"></a>
 
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</div>
 
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<h3>Vortex</h3>
 
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<ul>
 
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<li>Scientific Industries VortexGenie®2</li>
 
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<a href="http://www.scientificindustries.com/genie2.html"><p>for further information click here</p></a>
 
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<h3>Balance</h3>
 
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<ul>
 
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<li>MettlerToledo XS4002S DeltaRange® Balance</li>
 
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<a href="http://us.mt.com/us/en/home/products/Laboratory_Weighing_Solutions/Precision_Balances/XS_Precision_Balances/XS_4002_S_Dual_Range.html"><p>for further information click here</p></a>
 
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</html>
 
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<h3>Incubator</h3>
 
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<ul>
 
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<li>Thermo Scientific Heraeus Incubator</li>
 
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<a href="https://webshop.fishersci.com/insight2_uk/getProduct.do?productCode=11626250&resultSetPosition=0"><p>for further information click here</p></a>
 
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</html>
 
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[[File:Incubator.JPG|center|thumb]]
 
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<h3>FACS</h3>
 
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<ul>
 
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<li>BD LSRFortessa™ cell analyzer</li>
 
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<a href="http://www.bdbiosciences.com/us/instruments/research/cell-analyzers/bd-lsrfortessa/m/751752?cc=US"><p>for further information click here</p></a>
 
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<h3>Fluorescence Scanner for Western Blots</h3>
 
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<ul>
 
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<li>ODYSSEY® CLx Infrared Imaging System</li>
 
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<a href="http://www.licor.com/bio/products/imaging_systems/odyssey/"><p>for further information click here</p></a>
 
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[[File:FluorescenceScanner.JPG|center|thumb]]
 
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<h3>Emulsifier</h3>
 
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<ul>
 
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<li>EmulsiFlex-C3 from AVESTIN, Inc. and AVESTIN Europe GmbH/li>
 
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<a href="http://www.avestin.com/gc3page.html"><p>for further information click here</p></a>
 
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Revision as of 15:37, 8 September 2015

"What I cannot create I do not understand."
- Richard Feynmann

Materials

Bacterial strains and Mammalian Cell Lines

TOP10

The bacterial strain we used as a base for all our transformations was TOP10.

A list of all strains we generated in the course of our project can be found here (link).

Jurkat Cells

Immortalized human T lymphocyte cell line. We used this cell line as a model line for cancer cells with elevated lactate production and TRAIL susceptibility (papers).

HL60

Cell line derived from acute myeloid leukemia. We used this cell line as a model line for cancer cells with elevated lactate production and TRAIL susceptibility (papers).

3T3

Murine fibroblast cell line. We used this cell line as a model line for healthy cells which are restistant to TRAIL and produce normal levels of lactate.

SK-BR-3

HER2-positive breast cancer cell line. We used this cell line to validate the chimeric receptors of the Stockholm iGEM team.

Machines

Nano Drop

  • Thermo Scientific NanoDrop® 2000 UV-Vis spectrophotometer combined with NanoDrop 2000 / 2000c Software

    • for further information click here

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Plate Reader

Absorbance Measurments

UV- and Dark Hood

Large Centrifuge

Small Centrifuge

Shaker

PCR Machine

Gel Electrophoresis

Eppi-shaker/Heatingblock

Vortex

Balance

Incubator

FACS

Fluorescence Scanner for Western Blots

Emulsifier

Buffers and media

TFP1

  • 100 mM RbCl
  • 50 mM MnCl2
  • 30 mM potassium acetate
  • 10 mM CaCl2
  • 15%glycerol

TFP2

  • 100 mM MOPS
  • 50 mM RbCl
  • 75 mM CaCl2
  • 15%glycerol

SOC medium

  • 20 g/L Tryptophane
  • 5 g/L Yeast extract
  • 0.5 g/L NaCl
  • 250 mM KCl
  • 1M MgCl2
  • 50% (w/v) sterile glucose
  • Filter the solution through a 0.2 µm filter.

3T3 medium

  • 500 mL D-MEM 1x
  • 50 mL FBS 100%
  • 5 mL GlutaMax 100x
  • 5 mL Penicillin (10k units/mL) /Streptomyocin (10k µg/mL )

Jurkat medium

  • 2 mL MEM 1x
  • 20 mL FBS 100%
  • 2 mL GlutaMax 100x
  • 0.4 mL Penicillin (10k units/mL) /Streptomyocin (10k µg/mL )
  • 200 mL RPMI

Annexin V binding buffer

  • 10 mM HEPES
  • 140 mM NaCl
  • 2.5 mM
  • 250 mM CaCl2
  • pH 7.4
  • Filter the solution through a 0.2 µm filter.

Chemicals

LB-Broth

Agarose

Agar

Glycerol

2-log DNA ladder

Taq DNA Ligase

Cutsmart Buffer

EcoRI

DpnI

Q5 Master Mix

dNTP Mix

Taq DNA Polymerase

AnnexinV-Alexa

Green DNA/RNA Dye

Streptavidin Beads

Red Gel Stain

Anti-Human-AnnexinV

Gel Loading Dye

T4 DNA Ligase

PstI

DNA Ligase Buffer

Ethanol

Kits

Plasmid Purification

  • Product: ZR Plasmid Miniprep ™-Classic
  • Company: Zymo Research
  • Lot: ZRC182892
  • Catalog No: D4016

Changes to the protocol included an extra step to dry the column before elution and the use of water for elution instead of elution buffer. For elution of large plasmids we used prewarmed water.

Gel Extraction

  • Product: ZymocleanTM Gel DNA Recovery Kit
  • Company: Zymo Research
  • Lot: ZRC183055
  • Catalog No: D4002

Changes to the protocol included an extra step to dry the column before elution and the use of water for elution instead of elution buffer. For elution of large plasmids we used prewarmed water.

Lactate Detection Kit

  • Product: L-Lactic Acid
  • Company: Megazyme
  • Lot:
  • Catalog No:

Changes to the protocol included an extra step to dry the column before elution and the use of water for elution instead of elution buffer. For elution of large plasmids we used prewarmed water.

The detection of lactate using this kit relies on two enzymatic reactions. In the first, L-lactate and NAD&sup+; were converted into pyruvate and NADH+H&sup+; by L-lactate dehydrogenase. In the coupled reaction, pyruvate is used with D-glutamate by the D-glutamate-pyruvate transaminase to have the equilibrium of the first reaction in favour of the pyruvate. The concentration of NADH is finally measured as the optical density at 340 nm (OD340).