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Latest revision as of 08:55, 10 September 2015

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Ni-NTA bead regeneration

LP to regenerate Ni-NTA beads after use and elution of bound proteins

material:
duration:

  • centrifuge beads in Lysis buffer 1 min @ 500 g, 4°C
  • discard supernatant
  • add 2 mL 0.5 M NaOH per 15 mL Falcon tube
  • resuspend beads by flicking against the tube
  • incubate 30 min on ice
  • centrifuge 1 min @ 500 g, 4°C
  • discard supernatant
  • resuspend beads im 20 % ethanol by flicking
  • store @ 4°C
,