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Latest revision as of 09:02, 10 September 2015
PDMS-PDITC
protocol for PDITC surface on flow cells
According to protocol from AG Roth
material: chemicals, used kits, …
duration: … min
Slide preparation
- Name the slide
- Clean slides by washing with ethanol and rubbing (per hand, with glove)
- flush with aqua dest.
- Dry with wafergun
- Activate in plasma generator: gas flow 80 L/h, time 1 min
APTES
- Prepare APTES-solution: for 5 slides:
- 18 mL ethanol (90%)
- 1 mL dd aqua (5%)
- 1 mL APTES (5%)
- Incubate slides over night at RT in slideholder (transparent, sealed with parafilm) filled with APTES-solution
- Wash each flow cell individually with ethanol
- immerse for 5 min in ethanol in slideholder
- quickly dispose ethanol and dry flow cells with wafergun
- Put slides in new slideholder and bake them in oven at 70 °C for 60-90 min
PDITC
- Prepare PDITC-solution: for 5 slides:
- pyridin 2.0 mL
- DMF 18.0 mL
- PDITC 40 mg
- Cool down slides in slidebox with wafergun (go close with gun)
- Incubate slides in slideholder for 2 h filled with PDITC-solution at RT
- Discard PDITC-solution
- Fill with ethanol and discard ethanol
- Wash 2 x 5 min in slideholder with ethanol
- Fill with ethanol and discard ethanol once more
- Dry with wafergun
- stock in N2 atmosphere in box wrapped with parafilm
- storage >5 days