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Latest revision as of 09:04, 10 September 2015

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Halosurface from ABCR

protocol for functionalizing glass slides with halotag ligand

material: (3-Chloropropyl)triethoxysilane, acetone, isopropanol, aqua dest.
duration: ca. 85 min

  1. Wash with acetone, isopropanol, aqua dest.
  2. Dry with nitrogengun
  3. Activate in plasma generator: gas flow: 60-80 L/h, time: 10 min
  4. Pipette 80 µL of (3-Chloropropyl)triethoxysilane-solution per glass sandwich
  5. Incubate for 1 h at RT
  6. Part the sandwiches and put in acetone for 30 s
  7. Wash with acetone (work fast!!)
  8. Dry with nitrogengun
  9. Storable ??
,