Difference between revisions of "Team:UMaryland/Description"
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| − | < | + | <p style="font-size:72px"><b>Safe and Inexpensive</b></style> |
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| − | < | + | <p style="font-size:72px"><u><b>Approaches to Advance Synthetic Biology</b></u></style> |
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<p style="font-size:20px">Alternative methods of plasmid maintenance and PCR amplification accelerate the construction of new biodesigns, reduce cost, and avoid environmental hazards. Plasmids are typically maintained in cells by encoding enzymes that hydrolyze or otherwise detoxify antibiotics added to the medium. However, this process carries an inherent risk for spreading antibiotic resistance to native bacterial populations through lateral gene transfer. The Hok-Sok toxin-antitoxin system, a natural internal maintenance cassette relying on internal mRNA silencing, presents an alternative to common antibiotic-based methods since it does not rely on exogenous drugs. We are also developing an integrated, microcontrolled thermocycler using common household components. Using nichrome wire and a motorized fan for air circulation, the programmable prototype is an inexpensive, versatile thermocycler or plate incubator. Because the material and construction costs are a fraction of dedicated instruments, the newly developed unit will find broad application among nascent synthetic biologists in underfunded environments.</p> | <p style="font-size:20px">Alternative methods of plasmid maintenance and PCR amplification accelerate the construction of new biodesigns, reduce cost, and avoid environmental hazards. Plasmids are typically maintained in cells by encoding enzymes that hydrolyze or otherwise detoxify antibiotics added to the medium. However, this process carries an inherent risk for spreading antibiotic resistance to native bacterial populations through lateral gene transfer. The Hok-Sok toxin-antitoxin system, a natural internal maintenance cassette relying on internal mRNA silencing, presents an alternative to common antibiotic-based methods since it does not rely on exogenous drugs. We are also developing an integrated, microcontrolled thermocycler using common household components. Using nichrome wire and a motorized fan for air circulation, the programmable prototype is an inexpensive, versatile thermocycler or plate incubator. Because the material and construction costs are a fraction of dedicated instruments, the newly developed unit will find broad application among nascent synthetic biologists in underfunded environments.</p> | ||
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PCR is often a common tool in the laboratory environment. It is also a vital component in DNA amplification and any cloning procedure. However the majority of theses devices are bulky expensive machines that are rarely seen outside of bio based labs. This was the premise for the development of CHIP or Cheap Homemade Innovative PCR, development of a compact PCR machine that was as fast and proficient at amplifying DNA as lab based machines but for the fraction of the price and for teaching based applications. We are proud to say that we have been able to design a machine that will be completely open source and able to serve as a platform for DIY cloning procedures. | PCR is often a common tool in the laboratory environment. It is also a vital component in DNA amplification and any cloning procedure. However the majority of theses devices are bulky expensive machines that are rarely seen outside of bio based labs. This was the premise for the development of CHIP or Cheap Homemade Innovative PCR, development of a compact PCR machine that was as fast and proficient at amplifying DNA as lab based machines but for the fraction of the price and for teaching based applications. We are proud to say that we have been able to design a machine that will be completely open source and able to serve as a platform for DIY cloning procedures. | ||
Revision as of 16:50, 11 September 2015
