Difference between revisions of "Team:Pretoria UP/Parts"
| Line 43: | Line 43: | ||
<h3> BBa_K1768000 </h3> | <h3> BBa_K1768000 </h3> | ||
| + | <h5> GFP with RBS Reverse Compliment </5> | ||
<h4> Description </h4> | <h4> Description </h4> | ||
<p> | <p> | ||
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<h3> BBa_K1768001 </h3> | <h3> BBa_K1768001 </h3> | ||
| − | + | <h5> Recombinase invertible constitutive promoter 1 </5> | |
<h4> Description </h4> | <h4> Description </h4> | ||
<p> | <p> | ||
| Line 82: | Line 83: | ||
<h3> BBa_K1768002 </h3> | <h3> BBa_K1768002 </h3> | ||
| + | <h5> Quorum sensing-inducible mxiE transcription factor </5> | ||
<h4> Description </h4> | <h4> Description </h4> | ||
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<h3> BBa_K1768003 </h3> | <h3> BBa_K1768003 </h3> | ||
| + | <h5> Recombinase invertible constitutive promoter 2 </5> | ||
<h4> Description </h4> | <h4> Description </h4> | ||
A reverse compliment of a constitutive promoter flanked by Lox71 and Lox66 which are recognised by the Cre enzyme and facilitates a single round of recombination between these sites. Thus the direction of the promoter can be changed allowing expression of a different gene than in the initial state. This part is the same as part BBa_K1768001 but Lox71 is upstream of the promoter with a downstream lox66. | A reverse compliment of a constitutive promoter flanked by Lox71 and Lox66 which are recognised by the Cre enzyme and facilitates a single round of recombination between these sites. Thus the direction of the promoter can be changed allowing expression of a different gene than in the initial state. This part is the same as part BBa_K1768001 but Lox71 is upstream of the promoter with a downstream lox66. | ||
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<h3> Part:BBa_K1768002 </h3> | <h3> Part:BBa_K1768002 </h3> | ||
| + | <h5> Cre with N-terminal His tag </5> | ||
<h4> Description </h4> | <h4> Description </h4> | ||
<p> | <p> | ||
Revision as of 12:16, 17 September 2015
Part Documentation
Part Table
<groupparts>iGEM15 Pretoria_UP</groupparts>BBa_K1768000
GFP with RBS Reverse Compliment
Description
The reverse compliment of Green fluorescent protein (GFP) Part:BBa_E0040 and and upstream Ribosome Binding Site (RBS) Part:BBa_B0034
Characterization
Other
BBa_K1768001
Recombinase invertible constitutive promoter 1
Description
A reverse compliment of a constitutive promoter flanked by Lox66 and Lox71 which are recognised by the Cre enzyme and facilitates a single round of recombination between these sites. Thus the direction of the promoter can be changed allowing expression of a different gene than in the initial state. This part is the same as part BBa_K1768003 but Lox66 is upstream of the promoter with a downstream lox71.
Characterization
Other
BBa_K1768002
Quorum sensing-inducible mxiE transcription factor
Description
The LuxR receptor binds to the lux pR promoter to induce expression of the mxiE transcription factor (BBa_K1325001). mxiE forms a protein-protein interaction with the chaperone protein ipgC (BBa_K1325000) in order to transactivate the pipaH promoter (BBa_K1325004) via an "AND" operation.
Characterization
Other
BBa_K1768003
Recombinase invertible constitutive promoter 2
Description
A reverse compliment of a constitutive promoter flanked by Lox71 and Lox66 which are recognised by the Cre enzyme and facilitates a single round of recombination between these sites. Thus the direction of the promoter can be changed allowing expression of a different gene than in the initial state. This part is the same as part BBa_K1768001 but Lox71 is upstream of the promoter with a downstream lox66.
Characterization
Other
Part:BBa_K1768002
Cre with N-terminal His tag
Description
The Cre recombinase protein open reading frame with an N-terminal His protein as an epitope for antibody recognition.
Characterization
Other